Near‐Infrared Fluorescent Molecular Probe for Sensitive Imaging of Keloid

Abstract

Early detection of skin diseases is imperative for their effective treatment. However, fluorescence molecular probes that allow this are rare. The first activatable near‐infrared (NIR) fluorescent molecular probe is reported for sensitive imaging of keloid cells, skin cells from abnormal scar fibrous lesions. As keloid cells have high expression levels of fibroblast activation protein‐alpha (FAPα), the probe (FNP1) is designed to have a caged NIR dye and a FAPα‐cleavable peptide substrate linked by a self‐immolative segment. FNP1 can quickly and specifically turn on its fluorescence at 710 nm by 45‐fold in the presence of FAPα, allowing it to effectively recognize keloid cells from normal skin cells. Integration of FNP1 with a simple microneedle‐assisted topical application enables sensitive detection of keloid cells in metabolically‐active human skin tissue with a theoretical limit of detection down to 20 000 cells.

Bioluminescent Probe for Monitoring Endogenous Fibroblast Activation Protein-Alpha

“…So far, there were several fluorescent probes utilized for FAP detection. − For example, Ma reported a fluorescent probe for FAP and applied it for confocal imaging of FAP in living cells . Pu developed one for successfully imaging the overexpression of FAP in wound borders of keloids, while Wu described one for tumor imaging in nude mice model …”

Er3+ Sensitized 1530 nm to 1180 nm Second Near‐Infrared Window Upconversion Nanocrystals for In Vivo Biosensing

“…Owing to the well processability of PCL matrix, the sensor can be easily processed into various shapes.A samodel experiment, microneedle patches were fabricated for imaging of skin inflammation by casting PCL, NIR-II UCNPs,IR1061, and Fe 2+ complex solution into inverted polydimethylsiloxane (PDMS) mold to form the needle tips ( Figure 4A). [10] At op layer of pure PCL solution was added drop-wise onto the mold to create as table substrate (Supporting Information, Figure S18). Theobtained microneedles are arranged in a10 10 array with an area of 8 8mm.…”

A near-infrared fluorescence probe for imaging of pantetheinase in cells and mice in vivo

“…16 To facilitate the recognition moiety of the probe approaching the catalytic site, a self-immolative linker, 4-aminobenzyl methyl(2-(methylamino)-ethyl)carbamate, was inserted between the bulky uorophore and pantothenic acid to obtain the probe CYLP. 17,18 To our delight, this indirect construction strategy signicantly improved the analytical performance of the resulting probe, i.e., CYLP exhibited outstanding selectivity and sensitivity toward pantetheinase with NIR emission wavelength (>700 nm). Therefore, the uorescence imaging of pantetheinase activity could be achieved in vivo.…”