Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress

Huang, Yan; Yu, Fabiao; Wang, Jianchao; Chen, Lingxin

doi:10.1021/acs.analchem.6b00458

Cited by 158 publications

(84 citation statements)

References 53 publications

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“…[22][23][24][57][58][59] However, suitable fluorophores for tumor imaging must possess good hydrophilicity, stable photostability, high quantum yield, and excellent sensitivity in biological systems. Recent developments in cyanine dyes, squaraine, phthalocyanines, porphyrin derivatives, and BODIPY dyes have made them good candidates for tumor imaging (Fig.…”

Section: Nir Imaging Fluorophoresmentioning

confidence: 99%

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

et al. 2017

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Surgical resection of solid tumors is currently the gold standard and preferred therapeutic strategy for cancer. Chemotherapy drugs also make a significant contribution by inhibiting the rapid growth of tumor cells and these two approaches are often combined to enhance treatment efficacy. However, surgery and chemotherapy inevitably lead to severe side effects and high systemic toxicity, which in turn results in poor prognosis. Precision medicine has promoted the development of treatment modalities that are developed to specifically target and kill tumor cells. Advances in in vivo medical imaging for visualizing tumor lesions can aid diagnosis, facilitate surgical resection, investigate therapeutic efficacy, and improve prognosis. In particular, the modality of fluorescence imaging has high specificity and sensitivity and has been utilized for medical imaging. Therefore, there are great opportunities for chemists and physicians to conceive, synthesize, and exploit new chemical probes that can image tumors and release chemotherapy drugs in vivo. This review focuses on small molecular ligand-targeted fluorescent imaging probes and fluorescent theranostics, including their design strategies and applications in clinical tumor treatment. The progress in chemical probes described here suggests that fluorescence imaging is a vital and rapidly developing field for interventional surgical imaging, as well as tumor diagnosis and therapy.

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Section: Nir Imaging Fluorophoresmentioning

confidence: 99%

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

et al. 2017

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“…Utilizing the successive cascade reactions mediated by O 2 .− and polysulfides (H 2 S n ), Chen et al. reported cyanine‐based fluorescent probes 35 and 36 for simultaneous detection of mitochondrial O 2 .− and H 2 S n (Figure ) . The lipophilic cation and benzyl moiety can promote probe accumulation in mitochondria.…”

Section: Current Status Of Fluorescent Probes For O2−mentioning

confidence: 99%

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

et al. 2019

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The superoxide anion (O2.−) is widely engaged in the regulation of cell functions and is thereby intimately associated with the onset and progression of many diseases. To ascertain the pathological roles of O2.− in related diseases, developing effective methods for monitoring O2.− in biological systems is essential. Fluorescence imaging is a powerful tool for monitoring bioactive molecules in cells and in vivo owing to its high sensitivity and high temporal‐spatial resolution. Therefore, increasing numbers of fluorescent imaging probes have been constructed to monitor O2.− inside live cells and small animals. In this minireview, we summarize the methods for design and application of O2.−‐responsive fluorescent probes. Moreover, we present the challenges for detecting O2.− and suggestions for constructing new fluorescent probes that can indicate the production sites and concentration changes in O2.− as well as O2.−‐associated active molecules in living cells and in vivo.

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“…Yu and colleagues have reported a fluorescent probe for both superoxide anion and hydrogen polysulfides in mitochondria, Hcy-Mito (35). When HUVECs were incubated with 1 μ M Hcy-Mito for 15 min, the cells did not show any significant fluorescence increase, and the apoptosis rate was almost 0%.…”

Section: Cellular Applications Of Fluorescent Probes and Inhibitors Imentioning

confidence: 99%

Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling

Takano

Echizen

Hanaoka

2017

Antioxidants & Redox Signaling

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Significance: Hydrogen sulfide (H2S) plays roles in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. Also, hydropersulfide (R−S−SH) and polysulfide (−S−Sn−S−) have recently been identified as reactive sulfur species (RSS) that regulate the bioactivities of multiple proteins via S-sulfhydration of cysteine residues (protein Cys−SSH) and show cytoprotection. Chemical tools such as fluorescent probes and selective inhibitors are needed to establish in detail the physiological roles of H2S and polysulfide.Recent Advances: Although many fluorescent probes for H2S are available, fluorescent probes for hydropersulfide and polysulfide have only recently been developed and used to detect these sulfur species in living cells.Critical Issues: In this review, we summarize recent progress in developing chemical tools for the study of H2S, hydropersulfide, and polysulfide, covering fluorescent probes based on various design strategies and selective inhibitors of H2S- and polysulfide-producing enzymes (cystathionine γ-lyase, cystathionine β-synthase, and 3-mercaptopyruvate sulfurtransferase), and we summarize their applications in biological studies.Future Directions: Despite recent progress, the precise biological functions of H2S, hydropersulfide, and polysulfide remain to be fully established. Fluorescent probes and selective inhibitors are effective chemical tools to study the physiological roles of these sulfur molecules in living cells and tissues. Therefore, further development of a broad range of practical fluorescent probes and selective inhibitors as tools for studies of RSS biology is currently attracting great interest. Antioxid. Redox Signal. 27, 669–683.

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Near-Infrared Fluorescence Probe for in Situ Detection of Superoxide Anion and Hydrogen Polysulfides in Mitochondrial Oxidative Stress

Cited by 158 publications

References 53 publications

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

Fluorescent chemical probes for accurate tumor diagnosis and targeting therapy

Versatile Fluorescent Probes for Imaging the Superoxide Anion in Living Cells and In Vivo

Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling

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