Near-infrared fluorescence imaging with indocyanine green-lactosomes in a mouse model of rheumatoid arthritis

Onishi, Shinzo; Sakane, Masataka; Tsukanishi, Toshinori; Funayama, Toru; Ozeki, Eiichi; Hara, Isao; Yamazaki, Masashi

doi:10.3109/14397595.2016.1170946

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…But the initial time and peak time of fluorescence signal in ICG-lactosomes occur much later than those in LPs, which may be attributed to the ability of cells to internalize liposomes more rapidly over that to internalize lactosomes. 30,31 Taken together, the above mentioned findings further explain the reason for rapid aggregation of iLPs in the inflamed paws within just 15 min.…”

Section: Discussionsupporting

confidence: 57%

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes

Wu¹,

Wu²,

He³

et al. 2018

IJN

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BackgroundRheumatoid arthritis (RA) is a common inflammatory disorder characterized primarily by synovitis and pannus formation in multiple joints, causing joints destruction and irreversible disability in most cases. Early diagnosis and effective therapy monitoring of RA are of importance for achieving the favorable prognosis.MethodsWe first prepared the targeted fluorescence probes, and then explored the feasibility of near-infrared (NIR) fluorescence molecular imaging to detect and evaluate the RA via the targeted fluorescence probes by quantitative analysis in this study.ResultsThe targeted fluorescence probes (indocyanine green-liposomes decorated with iRGD peptide [iLPs]) was successfully prepared. The quantitative analysis found that strong fluorescence signal was detected in inflamed paws and the fluorescence signal in iLPs group was 3.03-fold higher than that in non-targeted (indocyanine green-liposomes decorated without iRGD peptide [LPs]) group (P<0.01) at 15 min after injection, whereas the fluorescence signal from iLPs signal can almost not be observed in the non-inflamed paws, showing the high sensitivity and accuracy for arthritis by the NIR fluorescence imaging based on iLPs.ConclusionThe NIR fluorescence imaging by iLPs may facilitate improved arthritis diagnosis and early assessment of the disease progression by providing an in vivo characterization of angiogenesis in inflammatory joint diseases.

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Section: Discussionsupporting

confidence: 57%

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes

Wu¹,

Wu²,

He³

et al. 2018

IJN

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“…Previous reports indicate that Near-infrared fluorescence imaging system with ICG show significant accumulation in inflamed joints with enhanced permeability and retention (EPR), like as the accumulation observed in tumors [29, 30]. Blood vessel insufficiency of inflamed joints in arthritic experimental models increases the leakiness and permeability, which enhances the accumulation of liposomes in arthritic lesion by EPR [31].…”

Section: Methodsmentioning

confidence: 99%

The effect of anti-IL-6 receptor antibody for the treatment of McH-lpr/lpr-RA1 mice that spontaneously developed destructive arthritis and enthesitis

Izumiyama

Mori

et al. 2019

BMC Musculoskelet Disord

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Background McH-lpr/lpr-RA1 mice are a new strain of mice which spontaneously develop destructive arthritis and enthesitis in the ankle. There is no published data that drug treatment has been trialed on these mice. This study examined the effect of the mouse anti-IL-6 receptor antibody, MR16–1, for the treatment of arthritis and enthesitis in McH-lpr/lpr-RA1 mice. Methods Male McH-lpr/lpr-RA1 mice were randomly divided into control and treatment groups. MR16–1 was administered from 10 weeks of age for the treatment group. Saline was applied for the control group. The drug was administered once a week, at an initial dose of 2 mg, then maintained at 0.5 mg once per week thereafter. The effects were evaluated by the histopathological synovitis score, in vivo imaging using indocyanine green liposomes, and analysis of the gene expression of inflammatory cytokines. Results Tissue analyses were carried out at 14, 17 and 20 weeks of age. The synovitis scores of treated groups were significantly lower compared with those of the control group at 14 and 17 weeks of age. The kappa coefficient was 0.77. However, progression of entheseal ossification persisted in the MR16–1 treated group. In vivo imaging using indocyanine green liposomes showed significant decreases in signal intensities of treated groups at week 14, but no significant differences were observed at week 18. Blood serum amyloid A levels in treated groups were significantly lower at 17 weeks of age. The gene expression levels of Tnf and Il17 were also significantly lower in MR16–1 treated groups. Conclusions Administration of the anti-IL-6 receptor antibody is effective for the treatment of synovitis and bone destruction of McH-lpr/lpr-RA1 mice. McH-lpr/lpr-RA1 mice may be a suitable experimental model for the development of new treatments for destructive arthritis and enthesitis. IL-6 signal blockade could contribute to the treatment of destructive arthritis, and further studies should be carried out to confirm its potential in the prevention of enthesopathy developed to ossification.

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“…A similar halogen lamp is used for optical excitation and optical filters are used to collect images in different excitation/emission bands between 415 and 850 nm. This allows for multiplexed fluorescence imaging and also simultaneous fluorescence and bioluminescence imaging, as demonstrated by several groups studying oncological [ 49 , 50 ], cardiovascular [ 51 – 53 ], and rheumatic diseases [ 54 , 55 ]. FMT systems focus exclusively on NIR-I tomography, with up to 4 lasers (635, 670, 745, and 790 nm) for optical excitation.…”

Section: Instrumentation For Small-animal Fluorescence Imagingmentioning

confidence: 99%

In vivo fluorescence imaging: success in preclinical imaging paves the way for clinical applications

et al. 2022

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Advances in diagnostic imaging have provided unprecedented opportunities to detect diseases at early stages and with high reliability. Diagnostic imaging is also crucial to monitoring the progress or remission of disease and thus is often the central basis of therapeutic decision-making. Currently, several diagnostic imaging modalities (computed tomography, magnetic resonance imaging, and positron emission tomography, among others) are routinely used in clinics and present their own advantages and limitations. In vivo near-infrared (NIR) fluorescence imaging has recently emerged as an attractive imaging modality combining low cost, high sensitivity, and relative safety. As a preclinical tool, it can be used to investigate disease mechanisms and for testing novel diagnostics and therapeutics prior to their clinical use. However, the limited depth of tissue penetration is a major challenge to efficient clinical use. Therefore, the current clinical use of fluorescence imaging is limited to a few applications such as image-guided surgery on tumors and retinal angiography, using FDA-approved dyes. Progress in fluorophore development and NIR imaging technologies holds promise to extend their clinical application to oncology, cardiovascular diseases, plastic surgery, and brain imaging, among others. Nanotechnology is expected to revolutionize diagnostic in vivo fluorescence imaging through targeted delivery of NIR fluorescent probes using antibody conjugation. In this review, we discuss the latest advances in in vivo fluorescence imaging technologies, NIR fluorescent probes, and current and future clinical applications. Graphical Abstract

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Near-infrared fluorescence imaging with indocyanine green-lactosomes in a mouse model of rheumatoid arthritis

Abstract: NIR fluorescence imaging of ICG-lactosomes detects arthritic joints in a mouse model of RA. ICG-lactosomes may preferentially localize to inflamed joints via enhanced permeability and retention.

Cited by 3 publications

References 13 publications

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes

Synovitis in mice with inflammatory arthritis monitored with quantitative analysis of dynamic contrast-enhanced NIR fluorescence imaging using iRGD-targeted liposomes as fluorescence probes

The effect of anti-IL-6 receptor antibody for the treatment of McH-lpr/lpr-RA1 mice that spontaneously developed destructive arthritis and enthesitis

In vivo fluorescence imaging: success in preclinical imaging paves the way for clinical applications

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