Near-infrared, dual-ratiometric fluorescent label for measurement of pH

Povrozin, Yevgen A.; Маркова, Л. И.; Tatarets, Anatoliy L.; Sidorov, Vadim; Terpetschnig, Ewald; Patsenker, L. D.

doi:10.1016/j.ab.2009.03.047

Cited by 31 publications

(19 citation statements)

References 19 publications

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“…The deprotonated form has a very large Stokes shift, allowing for ratiometric measurement of pH. (Povrozin, et al, 2009) An interesting use of pH sensitive dyes is to deliver them to tumor cells and tissue. This requires the functionalization of the probes for subsequent conjugation to protein, peptides, or other molecules of interest.…”

Section: Commentarymentioning

confidence: 99%

Near‐Infrared Molecular Probes for In Vivo Imaging

et al. 2012

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Cellular and tissue imaging in the near‐infrared (NIR) wavelengths between 700 and 900 nm is advantageous for in vivo imaging because of the low absorption of biological molecules in this region. This unit presents protocols for small animal imaging using planar and fluorescence lifetime imaging techniques. Included is an overview of NIR fluorescence imaging of cells and small animals using NIR organic fluorophores, nanoparticles, and multimodal imaging probes. The development, advantages, and application of NIR fluorescent probes that have been used for in vivo imaging are also summarized. The use of NIR agents in conjunction with visible dyes and considerations in selecting imaging agents are discussed. We conclude with practical considerations for the use of these dyes in cell and small animal imaging applications. Curr. Protoc. Cytom. 60:12.27.1‐12.27.20. © 2012 by John Wiley & Sons, Inc.

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Section: Commentarymentioning

confidence: 99%

Near‐Infrared Molecular Probes for In Vivo Imaging

et al. 2012

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“…The dicyanomethylene dye conjugates of 3, 5, and 7 exhibit the longest lifetimes. An amine-reactive, pH-sensitive, long-wavelength ratiometric squaraine-based fluorescent label, Square-650-pH, having a pKa in the physiological pH range with the main absorption and emission in the NIR region was reported recently [103]. Importantly, both the protonated and deprotonated forms of the label are fluorescent, with the deprotonated form having an extremely large Stokes shift of more than 100 nm (Fig.…”

Section: Squarainesmentioning

confidence: 96%

Long-wavelength fluorescence lifetime labels

et al. 2011

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“…Due to this pK a -shift to the alkaline pH range, some of these norsquaraines are also useful as fluorescent labels as their spectral properties are not affected in the pH range below 7.5. Square-650-pH having a pK a in the physiological pH range (pK a ¼ 7.1 for free dye and the pK a $ 6.1 when labeled to an antibody) was recently introduced by SETA BioMedicals [119]. This dye is commercially available as a free carboxylic acid and a mono-NHS ester.…”

Section: Norcyanines and Norsquaraines: Ph-sensitive Probes And Labelsmentioning

confidence: 98%

Long-Wavelength Probes and Labels Based on Cyanines and Squaraines

Patsenker

Tatarets

Terpetschnig³

2010

Springer Series on Fluorescence

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In this review, we make an attempt to compare the characteristics and applications of red and near infrared cyanine and squaraine dyes used for biological research, biomedical assays, and high-throughput screening. While the favorable photophysical properties of cyanine dyes makes them predestined as covalent labels, the environmentally sensitive squaraine dyes are utilizable as both florescent probes and labels. Reducing the aggregation tendencies of these dyes in aqueous media seems to be one of the most promising ways to improve their brightness, fluorescence lifetimes, and photostability. Indolenine-based squaraines including ring-substituted squaraines exhibit great potential for the design of bright and sensitive fluorescent probes and labels with increased photostability.

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Near-infrared, dual-ratiometric fluorescent label for measurement of pH

Cited by 31 publications

References 19 publications

Near‐Infrared Molecular Probes for In Vivo Imaging

Near‐Infrared Molecular Probes for In Vivo Imaging

Long-wavelength fluorescence lifetime labels

Long-Wavelength Probes and Labels Based on Cyanines and Squaraines

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