Long-wavelength fluorescence lifetime labels

Patsenker, L. D.; Tatarets, Anatoliy L.; Povrozin, Yevgen A.; Terpetschnig, Ewald

doi:10.1007/s12566-011-0025-2

Cited by 26 publications

(11 citation statements)

References 140 publications

(146 reference statements)

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“…Considering that the radiative decay rate of the dye, γ 0 , is much smaller than the corresponding non‐radiative decay rates γ NR , and that the radiative decay rate of the LPB, γ Ph , is very large, being basically given by the photon lifetime in the cavity (few femtoseconds in our low‐Q cavity, which gives γ Ph = 1/0.05 ps −1 ), the radiative efficiencies of SQ and SQMC can be approximated, within the limits of the rate equations model used, by

Φ_{S Q} \approx γ_{0} / γ_{N R}

and

Φ_{S Q M C} \approx γ_{L} / 2 γ_{N R}

, respectively, giving an estimation for the enhancement factor

G_{e} = Φ_{S Q M C} / Φ_{S Q} \approx γ_{L} / 2 γ_{0}

. Assuming a value of γ L = 1/50 ps −1 , which is in agreement with previous theoretical works, and γ 0 = 1/2000 ps −1 , we obtain an enhancement factor G e ≈ 20…”

Section: Resultsmentioning

confidence: 99%

Polariton‐Induced Enhanced Emission from an Organic Dye under the Strong Coupling Regime

Ballarini

Giorgi

Gambino

et al. 2014

Advanced Optical Materials

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Exciton-polaritons in semiconductors are quasi-particles which have recently shown the capability to undergo phase transition into a coherent hybrid state of light and matter. The observation of such quasi-particles in organic microcavities has attracted increasing attention for their characteristic of reaching condensation at room temperature. In this work we demonstrate that the emission properties of organic polaritons do not depend on the overlap between the absorption and emission states of the molecule and that the emission dynamics are modified in the strong coupling regime, showing a significant enhancement of the photoluminescence intensity as compared to the bare dye. This paves the way to the investigation of molecules with large absorption coefficients but poor emission efficiencies for the realization of polariton condensates and organic electrically injected lasers by exploiting strong exciton-photon coupling regimes.2

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Φ_{S Q} \approx γ_{0} / γ_{N R}

and

Φ_{S Q M C} \approx γ_{L} / 2 γ_{N R}

, respectively, giving an estimation for the enhancement factor

G_{e} = Φ_{S Q M C} / Φ_{S Q} \approx γ_{L} / 2 γ_{0}

. Assuming a value of γ L = 1/50 ps −1 , which is in agreement with previous theoretical works, and γ 0 = 1/2000 ps −1 , we obtain an enhancement factor G e ≈ 20…”

Section: Resultsmentioning

confidence: 99%

Polariton‐Induced Enhanced Emission from an Organic Dye under the Strong Coupling Regime

Ballarini

Giorgi

Gambino

et al. 2014

Advanced Optical Materials

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“…Due to their good photochemical and photophysical properties, rhodamine dyes are widely used in modern far-field optical microscopy and nanoscopy. [3] Despite their long history and wide use, rhodamine dyes, their preparation and post-synthetic modifications have not been studied in depth, [3,4] particularly in the case of red-emitting dye 1 (Scheme 1) with promising spectral properties. Recently we reported the synthesis and properties of the red-emitting rhodamine dye KK114 (compound 2 a in Scheme 1), [5] which turned out to be a bright and photostable fluorescent marker in various optical microscopy and nanoscopy techniques.…”

Section: Introductionmentioning

confidence: 99%

Red‐Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy

Kolmakov

Wurm

Hennig

et al. 2012

Chemistry A European J

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Fluorescent dyes emitting red light are frequently used in conventional and super-resolution microscopy of biological samples, although the variety of the useful dyes is limited. We describe the synthesis of rhodamine-based fluorescent dyes with absorption and emission maxima in the range of 621-637 and 644-660 nm, respectively and demonstrate their high performance in confocal and stimulated emission depletion (STED) microscopy. New dyes were prepared by means of reliable chemical transformations applied to a rhodamine scaffold with three variable positions. They feature polarity, water solubility, variable net charges, improved stabilities of N-hydroxysuccinimidyl (NHS) esters, as well as large fluorescence quantum yields in dye solutions and antibody conjugates. The photophysical and imaging properties of dyes containing three different polar groups, namely primary phosphate, sulfonic acid (in two different positions), and hydroxyl were compared. A dye with two primary phosphate groups was explored as a valuable alternative to dyes with "classical" sulfonic acid groups. Due to the increased net charge of the phosphorylated dye (q=-4 at pH 8), it demonstrated a far better electrophoretic mobility compared with analogues with two sulfonic acid groups (q=-2). As an example, one fluorescent dye was designed to be especially convenient for practical use. It is characterized by sufficiently high chemical stability of the NHS ester, its simple isolation, handling, and solubility in aqueous buffers, as well as in organic solvents. All these features, accompanied by a zero net charge in conjugates, were accomplished by the introduction of hydrophilic groups of two types: two hydroxyl groups and one sulfonic acid residue.

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“…The remarkable properties of squaraines have received attention for applications in photoconductivity, solar cells, and nonlinear optics [17]–[19]. Squaraine-based fluorescent sensors have been developed for a variety of analytes including Ca 2+ [20], pH [21], protein and DNA, and squaraine-based labels exhibit an increase in fluorescence intensity and lifetime upon binding to biomolecules [22], [23]. The photostability of squaraine dyes is comparable to those of conventional cyanine dyes [23], but can be substantially increased by the synthesis of a squaraine-rotaxane [24], an interlocked structure wherein a macrocycle encases the electrophilic squarylium core, preventing its exposure to nucleophilic attack in solution (Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging

Podgorski

Terpetschnig²,

Klochko

et al. 2012

PLoS ONE

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Fluorescent dyes that are bright, stable, small, and biocompatible are needed for high-sensitivity two-photon imaging, but the combination of these traits has been elusive. We identified a class of squaraine derivatives with large two-photon action cross-sections (up to 10,000 GM) at near-infrared wavelengths critical for in vivo imaging. We demonstrate the biocompatibility and stability of a red-emitting squaraine-rotaxane (SeTau-647) by imaging dye-filled neurons in vivo over 5 days, and utility for sensitive subcellular imaging by synthesizing a specific peptide-conjugate label for the synaptic protein PSD-95.

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Long-wavelength fluorescence lifetime labels

Cited by 26 publications

References 140 publications

Polariton‐Induced Enhanced Emission from an Organic Dye under the Strong Coupling Regime

Polariton‐Induced Enhanced Emission from an Organic Dye under the Strong Coupling Regime

Red‐Emitting Rhodamines with Hydroxylated, Sulfonated, and Phosphorylated Dye Residues and Their Use in Fluorescence Nanoscopy

Ultra-Bright and -Stable Red and Near-Infrared Squaraine Fluorophores for In Vivo Two-Photon Imaging

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