NCL-CD10–270: A New Monoclonal Antibody Recognizing CD10 in Paraffin-Embedded Tissue

McIntosh, Gary; Lodge, Andrew J.; Watson, Peter H.; Hall, Andrew G.; Wood, Katrina; Anderson, James; Angus, Brian; Horne, C. H. W.; Milton, Ian D.

doi:10.1016/s0002-9440(10)65253-4

Cited by 123 publications

(101 citation statements)

References 18 publications

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“…Sections were then allowed to cool in buffer solution for 20 min at the end of retrieval. Tissue from each patient was then immunostained for CD10 using Novocastra NCL-L-CD10-270 monoclonal antibody (Vector Laboratories, Peterborough, UK) at a 1:150 dilution 16 and the DAKO ChemMate system (DAKO K5001, horseradish peroxidase (HRP) mouse/rabbit with diaminobenzidine chromogen; DAKO, Ely, UK). In all cases, immunostaining for two additional canalicular antigens, the type IV integral membrane protein and ATP-binding cassette transporter bile salt export pump (BSEP; polyclonal antibody raised in rabbit; 1:50 dilution; HRP rabbit/goat; a generous gift from Y Meier and B Stieger, University of Zürich), 17 and the type II integral membrane protein alanyl aminopeptidase (CD13; Novocastra NCL-CD13-304; Vector Laboratories; dilution 1:200; HRP mouse/rabbit), was conducted under the same conditions as, and in sections parallel to, those used for CD10 immunostaining.…”

Section: Microscopy and Immunohistochemical Studiesmentioning

confidence: 99%

Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome

Byrne

Meara

Rayner

et al. 2007

Laboratory Investigation

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Many tissues, including hepatobiliary cells, express neutral endopeptidase (CD10), encoded by MME. Serum neutral endopeptidase activity (NEA) has been recommended as a marker of cholestasis in adults but not in children with Alagille syndrome (AGS). We investigated ontogenic and disease-related differences in the expression of CD10. CD10 was found on canalicular surfaces of hepatocytes throughout the lobule in 16 adults and in 31 children aged Z24 months, with and without cholestasis, but not in 39 children aged o24 months, with and without cholestasis. Ten AGS children aged 2 months to 6 years lacked any canalicular CD10 expression. Cholangiocyte apices and/or intrasinusoidal granulocytes marked for CD10 in all subjects. Liver membrane fractions from a child with cholestasis aged o24 months and from 2 AGS patients aged 424 months contained reduced levels of CD10. In contrast, AGS children and all controls expressed CD10 similarly on granulocytes. MME mRNA was found in the liver of children aged o24 months and of adults, all with cholestasis, and of AGS patients. Granulocyte MME mRNA levels were similar among all study subjects; however, liver MME mRNA levels were 6-to 140-fold less than in normal adults in all cholestatic subjects, including AGS children. Methylation of the MME promoter was not detected in the liver of AGS children. In conclusion, hepatocytes in early childhood physiologically lack immunohistochemically detectable CD10. Reduced MME mRNA in AGS is not due to MME promoter methylation. Liver CD10 in childhood appears to undergo reduced synthesis or rapid degradation, which persists in AGS. Absence of CD10 expression thus may limit NEA as a marker of cholestasis in young patients and in AGS.

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Section: Microscopy and Immunohistochemical Studiesmentioning

confidence: 99%

Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome

Byrne

Meara

Rayner

et al. 2007

Laboratory Investigation

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“…[1][2][3] CD10, a cell surface metalloproteinase localized to the proximal nephron of the normal adult kidney, 4 has been proposed as a useful tool in the differential diagnosis of renal epithelial neoplasms. [5][6][7] This molecule has been described as a positive immunohistochemical marker for the two most common types of kidney cancer, clear cell and papillary renal cell carcinomas.…”

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confidence: 99%

CD10 is expressed in a subset of chromophobe renal cell carcinomas

et al. 2004

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CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas. On the other hand, chromophobe renal cell carcinoma expresses parvalbumin, which is absent in clear cell and papillary renal cell carcinomas. To further address the relevance of these markers, we studied the expression of CD10 and parvalbumin in 42 samples of chromophobe renal cell carcinoma (seven of which had aggressive features, including invasion beyond the renal capsule, renal vein invasion, metastases, or sarcomatoid transformation), 75 clear cell renal cell carcinomas (eight metastatic) and 51 papillary renal cell carcinomas (two metastatic). CD10 was found in 100% of clear cell renal cell carcinomas, 63% of papillary renal cell carcinomas and in all metastatic cases of both types. At variance with previous studies, we found CD10 expression in from 30 to 90% of the neoplastic cells, in 11 of 42 (26%) chromophobe renal cell carcinomas. The CD10-positive cases included five of the seven (71%) chromophobe renal cell carcinoma with aggressive features. Statistical analysis showed significant association of CD10-positive tumors with clinicopathologic aggressiveness (P ¼ 0.003) and mitotic figures (P ¼ 0.04). Parvalbumin was strongly expressed in all primary and metastatic chromophobe renal cell carcinomas. Western blot analysis was utilized to confirm the expression of both CD10 and parvalbumin in chromophobe renal cell carcinomas. Keywords: chromophobe renal cell carcinoma; renal cell carcinoma; immunohistochemistry; CD10; parvalbumin The importance of distinguishing the different types of renal cell carcinoma is underscored by the fact that they have different prognoses. 1-3 CD10, a cell surface metalloproteinase localized to the proximal nephron of the normal adult kidney, 4 has been proposed as a useful tool in the differential diagnosis of renal epithelial neoplasms. [5][6][7] This molecule has been described as a positive immunohistochemical marker for the two most common types of kidney cancer, clear cell and papillary renal cell carcinomas. [5][6][7][8][9] Previous studies analyzing CD10 immunoreactivity in a total of 25 chromophobe renal cell carcinomas have found negative reactions, 5,7 whereas Holm-Nielsen et al 10 found CD10 expressed in all three chromophobe carcinomas studied and Higgins et al 11 found a positive reaction in one of three tumors tested. In this study we undertook to investigate CD10 and parvalbumin immunoreactivity in a large series of clear cell, papillary, and chromophobe renal cell carcinomas. Materials and methods Patients and Tissue SamplesWe studied the immunohistochemical expression of CD10 and parvalbumin in 75 clear cell renal cell carcinomas, 51 papillary renal cell carcinomas and 38 chromophobe renal cell carcinomas (including 36

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“…In the past, anti-CD10 antibodies were useful only in frozen-section immunophenotyping and flow cytometry; they lacked reactivity in fixed paraffinembedded tissue (3). Recently, CD10 immunostaining in paraffin-embedded tissue has been achieved with a newly generated monoclonal antibody (56C6; 9), which has been proven to be reactive with various cell types including renal tubules, glomeruli, brush borders of the intestine, hepatic canaliculi, syncytiotrophoblasts, and lymphoid germinal centers (9). MAb 56C6 has also been shown to be immunoreactive with a variety of neoplasms such as renal cell carcinoma, transitional cell carcinoma, prostatic adenocarcinoma, endometrial stromal sarcoma, rhabdomyosarcoma, pancreatic adenocarcinoma, schwannoma, and malignant melanoma (11).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, paraffin-reactive antibodies for CD10 have become commercially available (9). Immunohistochemistry (IHC) for CD10 is a practical method that is of particular value in retrospective studies of CD10 expression in paraffin-embedded tissue.…”

mentioning

confidence: 99%

Comparison of Multiparameter Flow Cytometry with Cluster Analysis and Immunohistochemistry for the Detection of CD10 in Diffuse Large B-Cell Lymphomas

McKenna

Kroft

2002

Modern Pathology

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CD10 is a critical antigen for the distinction of folliclecenter lymphoma from other B-cell lymphomas composed of small cells in fine-needle aspiration specimens, tissue core biopsies, and bone marrow. In addition, CD10 is expressed in a subset of diffuse large B-cell lymphomas (DLBCLs), where it may be an adverse prognostic indicator. We have previously demonstrated that CD10 expression detected by multiparameter flow cytometry (FC) with cluster analysis is highly sensitive and specific for follicle-center lymphoma in the differential diagnosis of small B-cell lymphomas. In this study, we assessed the utility of paraffin section immunohistochemistry (IHC) for CD10 compared with FC in a cohort of 50 DLBCLs. IHC for CD10 was technically successful in 47 of the 50 (94%) DLBCLs; 3 failed based on lack of internal CD10 reactivity. CD10 was expressed by FC in 20 of 47 DLBCLs (43%); CD10 was positive by IHC in 15 of these (75%). All 27 cases that were CD10(؊) by FC were negative by IHC. The level of CD10 expression by FC in the 5 FC(؉)/IHC(؊) cases ranged from relatively dim to bright. Our results indicate 75% sensitivity and 100% specificity of CD10 expression by IHC compared with multiparameter FC with cluster analysis and a 6% technical failure rate.

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NCL-CD10–270: A New Monoclonal Antibody Recognizing CD10 in Paraffin-Embedded Tissue

Cited by 123 publications

References 18 publications

Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome

Lack of hepatocellular CD10 along bile canaliculi is physiologic in early childhood and persistent in Alagille syndrome

CD10 is expressed in a subset of chromophobe renal cell carcinomas

Comparison of Multiparameter Flow Cytometry with Cluster Analysis and Immunohistochemistry for the Detection of CD10 in Diffuse Large B-Cell Lymphomas

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