Comparison of Multiparameter Flow Cytometry with Cluster Analysis and Immunohistochemistry for the Detection of CD10 in Diffuse Large B-Cell Lymphomas

Abstract

CD10 is a critical antigen for the distinction of folliclecenter lymphoma from other B-cell lymphomas composed of small cells in fine-needle aspiration specimens, tissue core biopsies, and bone marrow. In addition, CD10 is expressed in a subset of diffuse large B-cell lymphomas (DLBCLs), where it may be an adverse prognostic indicator. We have previously demonstrated that CD10 expression detected by multiparameter flow cytometry (FC) with cluster analysis is highly sensitive and specific for follicle-center lymphoma in the differential diagnosis of small B-cell lymphomas. In this study, we assessed the utility of paraffin section immunohistochemistry (IHC) for CD10 compared with FC in a cohort of 50 DLBCLs. IHC for CD10 was technically successful in 47 of the 50 (94%) DLBCLs; 3 failed based on lack of internal CD10 reactivity. CD10 was expressed by FC in 20 of 47 DLBCLs (43%); CD10 was positive by IHC in 15 of these (75%). All 27 cases that were CD10(؊) by FC were negative by IHC. The level of CD10 expression by FC in the 5 FC(؉)/IHC(؊) cases ranged from relatively dim to bright. Our results indicate 75% sensitivity and 100% specificity of CD10 expression by IHC compared with multiparameter FC with cluster analysis and a 6% technical failure rate.

Fine-needle cytology and flow cytometry immunophenotyping and subclassification of non-hodgkin lymphoma

“…The main advantages of FC immunophenotyping are the timely application of a complete panel of antibodies and contextual triage of fluorosceinated antibodies with detection of specific antibody expression and coexpression patterns that allow the application of diagnostic algorithms. 1,4 -10 Although these advantages cannot be overemphasized, many enthusiastic reports were based on different 4,9,23,28,29 or the evaluation of FC and immunocytochemical data together. 6,24,30 Moreover, in some experiences reported in the literature, cytopathologists performed both FNC and FC 12,16,22 ; in other reports, FC was performed in different laboratories by immunohematologists.…”

“…1,2,[13][14][15] The possibility of applying a complete panel of antibodies and diagnostic algorithms is the most appreciated advantage of the technique; 4,6,8 consequently, many reports stress the high sensitivity in detecting non-Hodgkin lymphoma (NHL) through the combined application of FNC and FC. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] The most enthusiastic reports hypothesize that, in the near future, histology will not be necessary strictly for the diagnosis of NHL. In fact, in many institutions, FNC coupled with ancillary techniques like FC routinely has replaced histology in the diagnosis of recurrent NHL (rNHL).…”

Flow cytometric features of angioimmunoblastic T‐cell lymphoma

“…Polymerase chain reaction (PCR) analysis of TCR gchain gene rearrangements, immunohistochemistry for CD10 and CD21, and in situ hybridization analysis of Epstein-Barr virus encoded RNA (EBER) were performed as previously described (12,13).…”

Increased Expression of the bcl6 and CD10 Proteins Is Associated with Increased Apoptosis and Proliferation in Diffuse Large B-Cell Lymphomas

“…Among hematopoietic cells, CD10 is expressed by immature B and T cells, by the germinal center B cells, by granulocytes, and by cells of a number of lymphoid malignancies (4,5,10,33). Regarding B-cell non-Hodgkin's lymphomas, CD10 protein is expressed in almost all Burkitt lymphomas, in most follicular B-cell lymphomas, and in a proportion of diffuse large B-cell lymphomas, whereas marginal zone/mucosa associated lymphoid tissue (MALT) lymphomas and mantle cell lymphomas are CD10 negative (10,24,(27)(28)(29)(30)(31)(32)(33)(34)(35).…”