NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility

Hu, Xin; Xing, Yishen; Fu, Xing; Yang, Qiyuan; Ren, Ling; Wang, Yahui; Li, Qian; Li, Junya; Zhang, Lupei

doi:10.3390/ijms21041248

Cited by 10 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biological and functional validation, including molecular function, in vitro, and in vivo studies, is critical for supporting GWAS results 34 and guiding the planning of follow‐up. In the present study, we attempted to verify an identified candidate gene in an in vitro functional study with proliferation and myogenic differentiation assays demonstrated to be reliable in vitro tools with which to investigate skeletal muscle development 50‐52 …”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we attempted to verify an identified candidate gene in an in vitro functional study with proliferation and myogenic differentiation assays demonstrated to be reliable in vitro tools with which to investigate skeletal muscle development. [50][51][52] factors. 7 MTPN was identified originally in a hypertensive rat heart model and has been shown to regulate growth of myocardial cells.…”

Section: Biological Function Validation Is Important In Post-gwas Studymentioning

confidence: 99%

See 1 more Smart Citation

Identification and validation of a novel candidate gene regulating net meat weight in Simmental beef cattle based on imputed next‐generation sequencing

Bordbar

Jensen

et al. 2020

Cell Proliferation

Self Cite

View full text Add to dashboard Cite

Objectives Genome‐wide association studies (GWAS) represent a powerful approach to detecting candidate genes for economically important traits in livestock. Our aim was to identify promising candidate muscle development genes that affect net meat weight (NMW) and validate these candidate genes in cattle. Materials and methods Using a next‐generation sequencing (NGS) dataset, we applied ~ 12 million imputed single nucleotide polymorphisms (SNPs) from 1,252 Simmental cattle to detect genes influencing net meat yield by way of a linear mixed model method. Haplotype and linkage disequilibrium (LD) blocks were employed to augment support for identified genes. To investigate the role of MTPN in bovine muscle development, we isolated myoblasts from the longissimus dorsi of a bovine foetus and treated the cells during proliferation, differentiation and hypertrophy. Results We identified one SNP (rs100670823) that exceeded our stringent significance threshold (P = 8.58 × 10−8) for a putative NMW‐related quantitative trait locus (QTL). We identified a promising candidate gene, myotrophin (MTPN), in the region around this SNP Myotrophin had a stimulatory effect on six muscle‐related markers that regulate differentiation and myoblast fusion. During hypertrophy, myotrophin promoted myotube hypertrophy and increased myotube diameters. Cell viability assay and flow cytometry showed that myotrophin inhibited myoblast proliferation. Conclusions The present experiments showed that myotrophin increases differentiation and hypertrophy of skeletal muscle cells, while inhibiting their proliferation. Our examination of GWAS results with in vitro biological studies provides new information regarding the potential application of myotrophin to increase meat yields in cattle and helpful information for further studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biological Function Validation Is Important In Post-gwas Studymentioning

confidence: 99%

Identification and validation of a novel candidate gene regulating net meat weight in Simmental beef cattle based on imputed next‐generation sequencing

Bordbar

Jensen

et al. 2020

Cell Proliferation

Self Cite

View full text Add to dashboard Cite

show abstract

“…NCAPG (non SMC condensin I complex subunit G) is a subunit of the lectin complex which is responsible for the concentration and stability of chromosomes during mitosis and meiosis and protein phosphorylation activation polysaccharide composition [7,8]. The dysregulation of NCAPG was associated with the occurrence of many diseases, including endometrial mixed adenocarcinoma and croup.…”

Section: Introductionmentioning

confidence: 99%

NCAPG Promotes the Proliferation of Renal Clear Cell Carcinoma via Mediating with CDK1

Zheng

et al. 2022

Disease Markers

View full text Add to dashboard Cite

Objective. Currently, lots of scholars have proved that the expression of NCAPG is associated with the prognosis of several cancers, while the relationship between NCAPG and renal clear cell carcinoma remains unclear, so the main aim of this research is to explore the effects of NCAPG on the progression of renal clear cell carcinoma. Methods. We observed the differential expression of NCAPG in several cancers from GEPIA online database, and the expression of NCAPG in renal clear cell carcinoma and normal tissue was compared and further verified by IHC assay. CCK-8 assay and clone formation experiment were conducted to observe the change of NCAPG on the proliferation. GraphPad was used for data analysis, and t -test and χ 2 analysis were used to analyze the correlation between NCAPG/CDK1 and renal clear cell carcinoma. Results. NCAPG was upregulated in renal clear cell carcinoma compared with the normal tissue, and the expression of NCAPG was associated with the clinical prognosis of pancreatic cancer especially with tumor size ( P = 0.010 ). Knockdown NCAPG could restrain the proliferation of renal clear cell carcinoma. CDK1 was found to be tightly related with NCAPG, and the expression of CDK1 was also associated with the prognosis. Conclusions. NCAPG was upregulated in renal clear cell carcinoma, which was related with tumor size and overall survival. NCAPG might promote the proliferation of renal clear cell carcinoma via mediating CDK1. NCAPG/CDK1 complex might provide a new treatment strategy for lots of patients with renal clear cell carcinoma.

show abstract

“…NCAPG may promote the occurrence and development of tumors by regulating the cell cycle, cell aging and mismatch repair [8]. NCAPG de ciency increases apoptosis and inhibits tumor cell proliferation [9]. Previous studies have shown that NCAPG is highly expressed in many malignant tumors, such as gastric cancer, liver cancer, glioma, and prostate cancer, and its expression level is closely related to the poor prognosis of the tumors [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of NCAPG in ovarian cancer is associated with ovarian cancer proliferation and apoptosis via p38 MAPK signaling pathway

Zou

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Non-SMC condensin I complex subunit G (NCAPG), a member of the subunit of condensin complex, is significantly overexpressed in various cancers and involved in the pathogenesis of cancers. However, the roles of NCAPG in ovarian cancer remain unclear. Methods The mRNA expression, overall survival and disease-free survival of NCAPG in ovarian cancer was analysed by GEPIA and KM plotter database, the expression levels of NCAPG in OC tissues and cell lines were determined by qPCR and immunohistochemistry analysis. ShRNA targeting NCAPG gene (sh-NCAPG) was utilized to knockdown NCAPG expression in OVCAR3 and SKOV3 cells. Subsequently, CCK-8 assay, colony formation assay, transwell invasion assay and flow cytometric analysis were performed to detect the effect of NCAPG on OC cell proliferation, apoptosis and invasion. Finally, Western blot assays were performed to detect the the mechanism of NCAPG in ovarian cancer. Results Analysis using GEPIA and KM plotter database showed NCAPG was upregulated in ovarian cancer and negatively associated with the survival of OC patients. qPCR and immunohistochemistry analysis confirmed it was highly expressed in both ovarian cancer tissues and cells. The silencing of NCAPG inhibited OC cell proliferation, invasion and induced cell apoptosis. Additionally, flow cytometric analysis revealed that NCAPG knockdown arrested the cell cycle at G2 and S phase. Furthermore, we also found that downregulation of NCAPG could suppress OC cell proliferation and invasion via activating p38 MAPK signaling pathway. Conclusion Our results suggest that NCAPG exhibits an important role in the development and progression of ovarian cancer and implicates NCAPG as a potential therapeutic target in ovarian cancer.

show abstract

NCAPG Dynamically Coordinates the Myogenesis of Fetal Bovine Tissue by Adjusting Chromatin Accessibility

Cited by 10 publications

References 49 publications

Identification and validation of a novel candidate gene regulating net meat weight in Simmental beef cattle based on imputed next‐generation sequencing

Identification and validation of a novel candidate gene regulating net meat weight in Simmental beef cattle based on imputed next‐generation sequencing

NCAPG Promotes the Proliferation of Renal Clear Cell Carcinoma via Mediating with CDK1

Overexpression of NCAPG in ovarian cancer is associated with ovarian cancer proliferation and apoptosis via p38 MAPK signaling pathway

Contact Info

Product

Resources

About