Naturally-Occurring Sendai Virus Infection of Athymic Nude Mice

Abstract

Abstract. Nude (nulnu) mice, Balb/c-derived, responded to a naturally-occurring Sendai virus infection in a different manner than conventional mice. They developed a chronic debilitating disease and a persistent viral infection of the respiratory tract with intranuclear inclusion bodies in tracheal, bronchial and bronchiolar epithelial cells, laryngeal and tracheal glandular epithelium and in type I and I1 alveolar cells. The infection was identified by serologic and tissue culture studies, the mouse antibody production test and ultrastructural examination of pulmonary lesions. Phlebitis of pulmonary veins, suppurative rhinitis and otitis media accompanied the viral infection while some mice developed a secondary bronchopneumonia.

Infectious Diseases of the Upper Respiratory Tract: Implications for Toxicology Studies

“…Sendai virus infection has been well described for mice (11)(12)(13), and recently the course of experimental infection in the rat has been reported (14). Sendai There are numerous systemic effects (Thble 1) that toxicologists and pathologists need to consider when interpreting the impact of Sendai virus infection upon study results (10,15).…”

Green Fluorescent Protein Gene Insertion of Sendai Virus Infection in Nude Mice: Possibility as an Infection Tracer.

“…progression of lung lesions with persistent SeV [4,12,20,23]. In this study, we compared the pathogenicity of recombinant SeV with an inserted GFP gene (GFP-SeV) with that of its recombinant wild-type SeV (Wt-SeV) to determine the usefulness of the recombinant virus application for studying viral pathogenicity.…”

“…In the lungs, SeV replicates in the bronchi-bronchiole epithelium and spreads to the alveolar wall, resulting in epithelial degeneration or proliferation accompanied by an inflammatory response of variable intensity, which is influenced by animal age, genotype, and immune status [3,5,11,13,17]. BALB/c nude (nu/nu) mice are known to be particularly susceptible to a wide range of infections that cause mild or no disease in normal (+/+) and heterozygotes (nu/+) mice [18,20,23]. A genetic deficiency in Tlymphocytes of the BALB/c nude mice causes the slow removed aseptically under ether anesthesia and exsanguination at 1, 2, 3, 5, 7, 10, 14, 30, 45 and 60 days after infection.…”

Signs and Lesions of Experimental Sendai Virus Infection in Two Genetically Distinct Strains of SCID/Beige Mice

“…21 Other opportunistic infections recognized to date include fatal septicemia associated with either Proteus mirabilis infectionsI9 or alpha hemolytic streptococcal infections1* and acute mortality associated with mouse hepatitis virus infe~tion.~ Sendai virus infections are relatively common in conventional colonies of laboratory mice,14 and depending on the circumstances, including genetic background, lesions may vary from minimal to m a~k e d .~.~ Athymic nude BALBlc mice are very susceptible to the disease. 22 The purpose of this study was to determine the degree of susceptibility of SCID-beige mice on either the C.B-17 or C57BL genetic background to Sendai virus. prepared from the lungs of inoculated mice, and this was used as our standard inoculum.…”