Naturally-derived targeted therapy for wound healing: Beyond classical strategies

El‐Ashram, Saeed; El-Samad, Lamia M.; Basha, Amal A.; Wakil, Abeer El

doi:10.1016/j.phrs.2021.105749

Cited by 54 publications

(33 citation statements)

References 126 publications

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“…Rapid wound healing and skin regeneration require the use of pharmaceuticals after a skin injury. Many herbal extracts and compounds have been demonstrated to have wound-healing effects on injured skin [17][18][19]27,[32][33][34]. Ginseng extract can also promote skin wound healing [17][18][19].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, controlling the biological events of these cell types may be important for promoting wound healing of the skin. Many extracts derived from herbal plants are known to have skin wound healing activity [17][18][19]27,[32][33][34]. However, the skin wound healing effect and mechanism of action of gintonin in keratinocytes have not been elucidated yet.…”

Section: Introductionmentioning

confidence: 99%

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Wound Healing Effect of Gintonin Involves Lysophosphatidic Acid Receptor/Vascular Endothelial Growth Factor Signaling Pathway in Keratinocytes

Choi

Won

Lee

et al. 2021

IJMS

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Gintonin, a novel compound of ginseng, is a ligand of the lysophosphatidic acid (LPA) receptor. The in vitro and in vivo skin wound healing effects of gintonin remain unknown. Therefore, the objective of this study was to investigate the effects of gintonin on wound healing-linked responses, especially migration and proliferation, in skin keratinocytes HaCaT. In this study, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide assay, Boyden chamber migration assay, scratch wound healing assay, and Western blot assay were performed. A tail wound mouse model was used for the in vivo test. Gintonin increased proliferation, migration, and scratch closure in HaCaT cells. It also increased the release of vascular endothelial growth factor (VEGF) in HaCaT cells. However, these increases, induced by gintonin, were markedly blocked by treatment with Ki16425, an LPA inhibitor, PD98059, an ERK inhibitor, 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis (acetoxymethyl ester), a calcium chelator, and U73122, a PLC inhibitor. The VEGF receptor inhibitor axitinib also attenuated gintonin-enhanced HaCaT cell proliferation. Gintonin increased the phosphorylation of AKT and ERK1/2 in HaCaT cells. In addition, gintonin improved tail wound healing in mice. These results indicate that gintonin may promote wound healing through LPA receptor activation and/or VEGF release-mediated downstream signaling pathways. Thus, gintonin could be a beneficial substance to facilitate skin wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Wound Healing Effect of Gintonin Involves Lysophosphatidic Acid Receptor/Vascular Endothelial Growth Factor Signaling Pathway in Keratinocytes

Choi

Won

Lee

et al. 2021

IJMS

View full text Add to dashboard Cite

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“…Any abnormality at these stages will negatively affect skin wound healing. With the extension of healing time, the dynamic system could be caught in a vicious circle of muscle spasm from local blood flow reduction, inflammatory [ 33 , 34 ]. Therefore, wound healing time is an important metric for choosing a hair removal method.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

Jia

Zhang

2022

Animals

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Hair removal is necessary before operating on animals with dense hair layers. To provide an appropriate hair removal method and maintain animal welfare, we introduced four commonly used depilation methods—namely, scissors shearing, electric shaving, depilatory cream, and sodium sulfide, and made systematic comparisons, instead of only examining one or two methods, as reported in the past. To further assess convenience and possible effects on skin wound healing, we performed a skin trauma model after depilation in C57BL/6J mice and recorded wound healing time. Meanwhile, the skin tissues around the wound were stained with H&E and Masson. The results showed that the wound contraction rate of the sodium sulfide group was significantly lower than other groups at different points in time. Furthermore, depilatory cream and sodium sulfide methods could induce a topical inflammatory response on the third day after the operation and delay the regeneration of collagen fibers. We concluded that sodium sulfide depilatory has a significant negative effect on wound healing. Depilatory cream is gentler, with mild skin irritation and symptoms of inflammation. The electric shaving method is more convenient and safer, and thus could be the best choice for preoperative depilation.

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“…Wounds represent the physical injuries that occur when the skin is broken externally or internally, which causes the opening or fracturing of skin. Various factors, such as temperature, oxygenation, and infections, as well as some other factors such as age, asthma, sex hormones, auto-immune disorders, smoking, obesity, etc., can interfere in wound healing [ 1 ]. It is a natural phenomenon that restores the integrity of the skin and also mitigates skin injury [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages

et al. 2022

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Wound healing is a complex biological phenomenon, having different but overlapping stages to obtained complete re-epithelization. The aim of the current study was to develop a dendrimer-based hydrogel bandage, to ameliorate full-thickness wounds. Hesperidin, a bioflavonoid found in vegetables and citrus fruits, is used for treatment of wounds; however, its therapeutic use is limited, due to poor water solubility and poor bioavailability. This issue was overcome by incorporating hesperidin in the inner core of a dendrimer. Hence, a dendrimer-based hydrogel bandage was prepared, and the wound healing activity was determined. A hemolysis study indicated that the hesperidin-loaded dendrimer was biocompatible and can be used for wound healing. The therapeutic efficacy of the prepared formulation was evaluated on a full-thickness wound, using an animal model. H&E staining of the control group showed degenerated neutrophils and eosinophils, while 10% of the formulation showed wound closure, formation of the epidermal layer, and remodeling. The MT staining of the 10% formulation showed better collagen synthesis compared to the control group. In vivo results showed that the preparation had better wound contraction activity compared to the control group; after 14 days, the control group had 79 ± 1.41, while the 10% of formulation had 98.9 ± 0.42. In a nutshell, Hsp-P-Hyd 10% showed the best overall performance in amelioration of full-thickness wounds.

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Naturally-derived targeted therapy for wound healing: Beyond classical strategies

Cited by 54 publications

References 126 publications

Wound Healing Effect of Gintonin Involves Lysophosphatidic Acid Receptor/Vascular Endothelial Growth Factor Signaling Pathway in Keratinocytes

Wound Healing Effect of Gintonin Involves Lysophosphatidic Acid Receptor/Vascular Endothelial Growth Factor Signaling Pathway in Keratinocytes

The Effect of Different Preoperative Depilation Ways on the Healing of Wounded Skin in Mice

Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages

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