Amelioration of Full-Thickness Wound Using Hesperidin Loaded Dendrimer-Based Hydrogel Bandages

Abstract

Wound healing is a complex biological phenomenon, having different but overlapping stages to obtained complete re-epithelization. The aim of the current study was to develop a dendrimer-based hydrogel bandage, to ameliorate full-thickness wounds. Hesperidin, a bioflavonoid found in vegetables and citrus fruits, is used for treatment of wounds; however, its therapeutic use is limited, due to poor water solubility and poor bioavailability. This issue was overcome by incorporating hesperidin in the inner core of a dendrimer. Hence, a dendrimer-based hydrogel bandage was prepared, and the wound healing activity was determined. A hemolysis study indicated that the hesperidin-loaded dendrimer was biocompatible and can be used for wound healing. The therapeutic efficacy of the prepared formulation was evaluated on a full-thickness wound, using an animal model. H&E staining of the control group showed degenerated neutrophils and eosinophils, while 10% of the formulation showed wound closure, formation of the epidermal layer, and remodeling. The MT staining of the 10% formulation showed better collagen synthesis compared to the control group. In vivo results showed that the preparation had better wound contraction activity compared to the control group; after 14 days, the control group had 79 ± 1.41, while the 10% of formulation had 98.9 ± 0.42. In a nutshell, Hsp-P-Hyd 10% showed the best overall performance in amelioration of full-thickness wounds.

Hesperidin - loaded PVA/alginate hydrogel: targeting NFκB/iNOS/COX-2/TNF-α inflammatory signaling pathway

“…The topical administration of H gel has demonstrated effective results in the treatment of wounds ( 10 , 62 , 63 ). The evaluation of H for its wound healing activity in irradiated mice is a significant research area with potential clinical implications ( 64 ). The study demonstrates that in γ-irradiated rats the topical administration of H accelerated the healing of wounds by increasing the collagen, hexosamine, DNA, and nitric oxide syntheses and increasing the densities of fibroblasts and blood vessels in the regenerating wounds ( 15 , 45 ).…”

Co-delivery of Tacrolimus and Thymoquinone topically by Nanostructured lipid carriers gel for enhanced efficacy against imiquimod-induced psoriasis in Balb/c mice

“…3B), revealed various speci c peaks associated with different stretching modes, such as C-H stretching, C = O stretching, and N-H bending vibrations in the case of skin treated with NLCs and untreated skin. These spectral features can be utilized to assess permeation dynamics [47]. These peaks correspond to the molecular vibrations of proteins and lipids present in the SC.…”

Local Drug Delivery Strategies towards Wound Healing

“…In addition to their structure, dendrimers may be the best drug delivery vehicles for many therapeutic treatments because of their size and lipophilicity, which allow them to easily permeate cell membranes. These distinguishing qualities have increased interest in using dendrimers nanoparticles for wound healing in research [ 158 ].…”

“…The structure of a chemical determines its toxicity as well as all of its other characteristics. A dendrimer’s toxicity can either be increased or decreased depending on specific components (core, branch, and surface groups) [ 158 , 161 ].…”