Naturally concurrent infections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms

Dong, Ha Thanh; Nguyen, Vuong Viet; Le, Hai Dinh; Sangsuriya, Pakkakul; Jitrakorn, Sarocha; Saksmerprome, Vanvimon; Senapin, Saengchan; Rodkhum, Channarong

doi:10.1016/j.aquaculture.2015.06.027

Cited by 156 publications

(160 citation statements)

References 41 publications

Supporting

Mentioning

121

Contrasting

Unclassified

Order By: Relevance

“…Motile aeromonads are very diverse, and not only A. hydrophila but other Aeromonas species have also been reported as threats to aquaculture freshwater fish (Dong et al . ; Eissa et al . ; Peepim et al .…”

Section: Discussionmentioning

confidence: 99%

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Dong

Techatanakitarnan

Jindakittikul

et al. 2017

Journal of Fish Diseases

199

156

View full text Add to dashboard Cite

Diseases caused by motile aeromonads in freshwater fish have been generally assumed to be linked with mainly Aeromonas hydrophila while other species were probably overlooked. Here, we identified two isolates of non-A. hydrophila recovered from Nile tilapia exhibiting disease and mortality after exposed to transport-induced stress and subsequently confirmed their virulence in artificial infection. The bacterial isolates were identified as Aeromonas jandaei and Aeromonas veronii based on phenotypic features and homology of 16S rDNA. Experimental infection revealed that the high dose of A. jandaei (3.7 × 10 CFU fish ) and A. veronii (8.9 × 10 CFU fish ) killed 100% of experimental fish within 24 h, while a 10-fold reduction dose killed 70% and 50% of fish, respectively. When the challenge dose was reduced 100-fold, mortality of the fish exposed to A. jandaei and A. veronii decreased to 20% and 10%, respectively. The survivors from the latter dose administration were rechallenged with respective bacterial species. Lower mortality of rechallenged fish (0%-12.5%) compared to the control groups receiving a primary infection (37.5%) suggested that the survivors after primary infection were able to resist secondary infection. Fish exposed to either A. jandaei or A. veronii exhibited similar clinical signs and histological manifestation.

show abstract

Section: Discussionmentioning

confidence: 99%

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Dong

Techatanakitarnan

Jindakittikul

et al. 2017

Journal of Fish Diseases

199

156

View full text Add to dashboard Cite

show abstract

“…Among the identified fish killers of cultivated tilapia in Thailand, a significant number of emerging infectious pathogens have been reported during recent years, notably a novel genetic group of Flavobacterium columnare (Dong et al, 2015a), Francisella noatunensis subsp. orientalis Jantrakajorn and Wongtavatchai, 2016;Nguyen et al, 2016), Hahella chejuensis , Aeromonas jandaei and Aeromonas veronii (Dong et al, 2015b;Dong et al, 2017), betanodavirus (Keawcharoen et al, 2015), and infectious spleen and kidney necrosis virus (ISKNV/Iridovirus) (Dong et al, 2015b;Suebsing et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection

et al. 2017

View full text Add to dashboard Cite

“…This study is the first to indicate that Piper betle extract appears to be very effective against V. parahaemolyticus, a pathogen that has assumed great significance recently by causing one of the most devastating disease outbreaks in the shrimp industry (Joshi et al, 2014;Tran et al, 2013). Piper betle extract has also been found to be effective against the four pathogenic bacteria that have been reported to cause diseases in tilapia and striped catfish including A. veronii, a newly reported pathogen in farmed t ilapia ( Dong et al, 2015a;Dong et al, 2016;Dong et al, 2015b ). Most of the previous studies lacked any in vivo evidence of the efficacy of Piper betle extract while presenting promising results of in vitro trials.…”

Section: Discussionmentioning

confidence: 87%

“…Two strains of Vibrio parahaemolyticus causing severe hepatopancreatic pathogenesis and mortality in shrimp (Joshi, et al, 2014) were obtained fro m BIOTEC/ NSTDA, Thailand. Four pathogenic bacterial isolates (Edwardsiella ictaluri, Edwardsiella tarda, Aeromonas veronii and Streptococcus agalactiae) causing diseases in freshwater fish were selected based on previous studies (Dong et al, 2015a;Dong et al, 2016;Donget al, 2015b). Prior to in vitro inhibition assay, bacteria were recovered fro m glycerol stocks by streaking onto indicated agar med iu m and incubated at 30ºC for 16 -32 hours (h).…”

Section: Bacterial Isolates and Culture Conditionmentioning

confidence: 99%

“…These include Streptococcus agalactiae, Aeromonas veronii, Edwardsiella ictaluri and Edwardsiella tarda affecting farmed tilapia (Oreochromis spp.) and striped catfish, Pangasianodon hypophthalmus (Dong et al, 2015a;Donget al, 2015b); and Vibrio parahaemolyticus causing acute hepatopancreatic necrosis diseases (AHPND) in white leg shrimp, Penaeus (Litopenaeus) vannamei (Joshi et al, 2014;Kongrueng et al, 2015;Tran et al, 2013). Streptococcus infections have an epidemiological importance in aquaculture because mortalities can be as h igh as 75% with potential horizontal and vertical (Pradeep et al, 2016) transmission.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Ataguba¹,

Dong²,

Rattanarojpong³

et al. 2018

Turk. J. Fish. Aquat. Sci.

Self Cite

View full text Add to dashboard Cite

An in vitro assessment of antimicrobial properties of aqueous and ethanol extracts from solo garlic (Allium sativum), garlic chive (Allium tuberosum) and betel leaves (Piper betle) on six bacterial pathogens in aquaculture, and a challenge of Nile tilapia, Oreochromis niloticus with Streptococcus agalactiae were performed. Generally, minimum inhibitory concentrations (M IC) ranged from 26.63 to 53.25 mg mL -1 for aqueous solo garlic (G) and 14.60 to 29.20 mg mL -1 for garlic chive extracts for all pathogens tested. Ethanol extract of betel leaves (P) exhibited the strongest antibacterial activity (0.15 -0.60 mg mL -1 ). P and G incorporated in feed at high and low doses as multiples of M IC [High; H (10X for PH and 3X for GH) and Low; L (3X for PL and 1X for GL)] were fed to tilapia followed by in vivo challenge against S. agalactiae (1 x 10 8 CFU mL -1 ). Ethanol extract of P. betle significantly improved survival (P<0.05; PH=100%, PL =77%). White blood cells (WBC), lymphocytes and monocytes differed significantly (P<0.05) among treatments and the highest WBC value (1.175 × 10 3 ) was for PH. Use of ethanol extract of Piper betle seems promising for sustainable disease management in aquaculture.

show abstract

Naturally concurrent infections of bacterial and viral pathogens in disease outbreaks in cultured Nile tilapia (Oreochromis niloticus) farms

Cited by 156 publications

References 41 publications

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Aeromonas jandaei and Aeromonas veronii caused disease and mortality in Nile tilapia, Oreochromis niloticus (L.)

Emergence of tilapia lake virus in Thailand and an alternative semi-nested RT-PCR for detection

Untitled

Contact Info

Product

Resources

About