Naturally-Acquired Dengue Virus Infections Do Not Reduce Short-Term Survival of Infected Aedes aegypti from Ho Chi Minh City, Vietnam

Carrington, Lauren B.; Nguyen, Hoa L.; Nguyen, Nguyet Minh; Duong, T. H. Kien; Tuan, Trung Vu; Giang, Nguyen Thi; Tuyet, Nhu Vu; Thi, Dui Le; Thi, Long Vo; Tran, Chau Nguyen; Simmons, Cameron P.

doi:10.4269/ajtmh.14-0499

Cited by 10 publications

(12 citation statements)

References 18 publications

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“…), consistent with the wide distribution of its main vector Aedes aegypti [9–12]. All the administrative levels report either autochthonous cases (>90% of the territories) or imported cases (as occurs in Bogota, the capital city).…”

Section: Introductionsupporting

confidence: 61%

“…No research has been conducted to assess the various factors which drive dengue transmission in this setting [20]. Those factors include education, economic income [21], migration and population flows [22], cross-protection between serotypes [12, 23], and the environmental factors which have influenced dengue epidemiology, including climate change [24, 25], and housing standards [26–28]. All of these are important in order to develop effective integrated interventions oriented to reduce transmission and the burden of disease in a given area.…”

Section: Introductionmentioning

confidence: 99%

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A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting

Martínez-Vega¹,

Rodríguez‐Morales

Bracho-Churio³

et al. 2016

BMC Infect Dis

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BackgroundDengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. In Colombia, dengue is a major public health problem. Departments of La Guajira, Cesar and Magdalena are dengue endemic areas. The objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these Departments, and also establish the association between individual and housing factors and vector indices with seroprevalence and incidence. We will also assess knowledge, attitudes and practices, and willingness-to-pay for dengue vaccine.MethodsA cohort study will be assembled with a clustered multistage sampling in 11 endemic municipalities. Approximately 1000 homes will be visited to enroll people older than one year who living in these areas, who will be followed for 1 year. Dengue virus infections will be evaluated using IgG indirect ELISA and IgM and IgG capture ELISA. Additionally, vector indices will be measured, and adult mosquitoes will be captured with aspirators. Ovitraps will be used for continuous estimation of vector density.DiscussionThis research will generate necessary knowledge to design and implement strategies with a multidimensional approach that reduce dengue morbidity and mortality in La Guajira and other departments from Colombian Caribbean.

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Section: Introductionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting

Martínez-Vega¹,

Rodríguez‐Morales

Bracho-Churio³

et al. 2016

BMC Infect Dis

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“…While some studies report no effect of DENV on fitness in A. aegypti (Carrington et al. ) others have measured reductions in survival and fecundity (Maciel‐de‐Freitas et al. ) and feeding and oviposition success (Sylvestre et al.…”

Section: Discussionmentioning

confidence: 99%

“…While some studies report no effect of DENV on fitness in A. aegypti (Carrington et al 2015) others have measured reductions in survival and fecundity (Maciel-de-Freitas et al 2011) and feeding and oviposition success (Sylvestre et al 2013). Such discrepancies are likely explained by differences in how fitness is measured and the virus × mosquito genotype combinations tested.…”

Section: Trade-offsmentioning

confidence: 99%

Evolutionary potential of the extrinsic incubation period of dengue virus inAedes aegypti

Chenoweth

Carrasco

et al. 2016

Evolution

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Dengue fever is the most common arboviral disease worldwide. It is caused by dengue viruses (DENV) and the mosquito Aedes aegypti is its primary vector. One of the most powerful determinants of a mosquito's ability to transmit DENV is the length of the extrinsic incubation period (EIP), the time it takes for a virus to be transmitted by a mosquito after consuming an infected blood meal. Here, we repeatedly measured DENV load in the saliva of individual mosquitoes over their lifetime and used this in combination with a breeding design to determine the extent to which EIP might respond to the evolutionary forces of drift and selection. We demonstrated that genetic variation among mosquitoes contributes significantly to transmission potential and length of EIP. We reveal that shorter EIP is genetically correlated with reduced mosquito lifespan, highlighting negative life-history consequences for virus-infected mosquitoes. This work highlights the capacity for local genetic variation in mosquito populations to evolve and to dramatically affect the nature of human outbreaks. It also provides the impetus for isolating mosquito genes that determine EIP. More broadly, our dual experimental approach offers new opportunities for studying the evolutionary potential of transmission traits in other vector/pathogen systems.

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“…Carrington et al . () reported that Ae. aegypti that acquired a dengue infection by feeding on hospitalized dengue patients, the most natural route of infection, did not show a difference in survival compared to mosquitoes that were not infected for a period of 12 days post‐feeding.…”

Section: Discussionmentioning

confidence: 99%

Rise and fall of vector infectivity during sequential strain displacements by mosquito‐borne dengue virus

Andrade

Young

Johnson

et al. 2016

J of Evolutionary Biology

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Each of the four serotypes of mosquito-borne dengue virus (DENV-1-4) comprises multiple, genetically-distinct strains. Competitive displacement between strains within a serotype is a common feature of DENV epidemiology and can trigger outbreaks of dengue disease. We investigated the mechanisms underlying two sequential displacements by DENV-3 strains in Sri Lanka that each coincided with abrupt increases in dengue hemorrhagic fever (DHF) incidence. First, the post-DHF strain, displaced the pre-DHF strain in the 1980s. We have previously shown that post-DHF is more infectious than pre-DHF for the major DENV vector, Aedes aegypti. Then, the ultra-DHF strain evolved in situ from post-DHF and displaced its ancestor in the 2000s. We predicted that ultra-DHF would be more infectious for Ae. aegypti than post-DHF but found that ultra-DHF infected a significantly lower percentage of mosquitoes than post-DHF. We therefore hypothesized that ultra-DHF had effected displacement by disseminating in Ae. aegypti more rapidly than post-DHF, but this was not borne out by a timecourse of mosquito infection. To elucidate the mechanisms that shape these virus-vector interactions, we tested the impact of RNA interference, the principal mosquito defense against DENV, on replication of each of the three DENV strains. Replication of all strains was similar in mosquito cells with dysfunctional RNAi, but in cells with functional RNAi, replication of pre-DHF was significantly suppressed relative to the other two strains. Thus differences in susceptibility to RNAi may account for the differences in mosquito infectivity between pre-DHF and post-DHF, but other mechanisms underlie the difference between post-DHF and ultra-DHF.

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Naturally-Acquired Dengue Virus Infections Do Not Reduce Short-Term Survival of Infected Aedes aegypti from Ho Chi Minh City, Vietnam

Cited by 10 publications

References 18 publications

A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting

A prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting

Evolutionary potential of the extrinsic incubation period of dengue virus inAedes aegypti

Rise and fall of vector infectivity during sequential strain displacements by mosquito‐borne dengue virus

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