Natural wax constituents of a supercritical fluid CO2 extract from quince (Cydonia oblonga Mill.) pomace

Abstract

The chemical constituents of a lipophilic extract from quince (Cydonia oblonga Mill.), obtained by supercritical fluid CO(2) extraction of the dried fruit pomace were investigated. Solvent partition of quince wax with n-hexane or acetone yielded an insoluble (crystalline) and a soluble (oily) fraction. Both fractions were analyzed separately using gas chromatography/mass spectrometry (GC/MS). The insoluble fraction consisted of saturated n-aldehydes, n-alcohols and free n-alkanoic acids of carbon chain lengths between 22 and 32, with carbon chain lengths of 26 and 28 dominating. Also odd-numbered unbranched hydrocarbons, mainly C27, C29 and C31, were detected particularly in the acetone-insoluble fraction (total, 15.8%). By means of vacuum liquid chromatography, triterpenoic acids were separated from the hexane-insoluble matter and identified as a mixture of ursolic, oleanolic and betulinic acids. The major constituents of the hexane-soluble fraction were glycerides of linoleic [Δ(9,12), 18:2] and oleic [Δ(9), 18:1] acids, accompanied by free linoleic, oleic and palmitic acids (C16). Moreover β-sitosterol, Δ(5)-avenasterol as well as trace amounts of other sterols were assigned. Finally the carotenoids phytoene and phytofluene were identified and quantified by UV/vis and high-performance liquid chromatography/MS techniques, yielding 1.0 and 0.3% of the quince wax, respectively. It is anticipated that the complex of lipid constituents from quince wax may exert interesting biological activities, the elucidation of which awaits further studies.

Identification of key odor volatile compounds in the essential oil of nine peach accessions

“…Although hydrocarbons and long‐chain aldehydes are not likely to play a significant role in odor composition, they could be important as a side‐product for the fruit industry. For example, production residues such as pomace could be exploited to obtain valuable ingredients for the cosmetics and food industries 23, 24…”

Studies into the phenolic patterns of different tissues of pineapple (Ananas comosus [L.] Merr.) infructescence by HPLC-DAD-ESI-MS n and GC-MS analysis

“…After the addition of 0.2 mL of the silylation reagent [14], the sample was flushed with nitrogen and heated to 105°C held for 45 min in a 10-mL Pyrex vial sealed with a PTFEcoated closure [20]. The solutions were allowed to cool to room temperature and centrifuged for 5 min at 5000×g prior to GC-MS analysis [21,22].…”

Cydonia oblonga M., A Medicinal Plant Rich in Phytonutrients for Pharmaceuticals

“…Analysis of lipophilic fractions (soluble and insoluble in n -hexane and acetone) obtained from dried Quince fruit was ended with conclusion that insoluble n -hexane extract consisted of n -alcohols, saturated n -aldehydes, and free alkanoic acids, triterpenoic acids (oleanolic, betulinic, and ursolic acids) whereas, n -hexane soluble portion contained glycerides of linoleic acid and oleic acid along with free palmitic acids, oleic, and linoleic acids. In acetone insoluble portion, odd numbered unbranched hydrocarbons (C27, C29, C31) were identified (Lorenz et al, 2008 ). Carotenoid cleavage enzyme from Quince fruit was isolated (Fleischmann et al, 2002 ).…”