Natural vertical transmission of dengue viruses in Aedes aegypt in southern India

Thenmozhi, V.; Tewari, S. C.; Manavalan, R.; Balasubramanian, A.; Gajanana, A.

doi:10.1016/s0035-9203(00)90067-1

Cited by 39 publications

(27 citation statements)

References 1 publication

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“…6,7 Many studies have shown that DENV could be transovarially transmitted to progeny mosquitoes both in the laboratory and field. [50][51][52][53][54][55][56][57][58][59][60] It was considered to be an important means for the maintenance of DENV in nature. However, the mechanism of transovarially transmission is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

Zhang

Zheng²,

Yu³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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Dengue virus serotype 2 (DENV-2) RNA replication profiles and tropisms were studied by using quantitative RT-PCR (q-RTPCR) in intrathoracically infected Aedes albopictus. The virus RNA replication profiles were diverse in mosquito organs. In fat body, brain, salivary gland, and malpighian tubes, it peaked at 8, 23, 23, and 27 days post-infection, respectively, and then, all declined. In midgut, it increased all the time and had no trend of decline. In ovary, it had no apparent increase. Subsequent Western blotting of DENV-2 E protein had similar results. Using ribosomal protein 7 (rpS7) as an internal control, we found that, in salivary gland, brain, fat body, and midgut, the average DENV-2 RNA levels (DENV-2 RNA/rpS7 mRNA) were 1,028, 464, 5.6, and 6.2, respectively; in malpighian tubes, it was 1, and in ovary, it was far less than 1. These results suggest that infection profiles and tropism of DENV-2 RNA in Ae. albopictus organs are significantly different.

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Section: Discussionmentioning

confidence: 99%

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

Zhang

Zheng²,

Yu³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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“…albopictus) is incriminated in dengue transmission [8,9] , Aedes aegypti (Ae. aegypti) remains so far the major vector [10] .…”

Section: Introductionmentioning

confidence: 99%

Household survey of container–breeding mosquitoes and climatic factors influencing the prevalence of Aedes aegypti (Diptera: Culicidae) in Makkah City, Saudi Arabia

Aziz

Dieng

Ahmad

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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“…Otra explicación de la persistencia del virus es la posibilidad de otras formas de transmisión y circulación del virus en el vector, por ejemplo, la transmisión transovárica o vertical (11,12).…”

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Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

et al. 2017

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Introducción. La incidencia y la prevalencia del dengue en Cundinamarca son elevadas y, recientemente, se detectó Aedes aegypti en algunas áreas rurales del departamento.Objetivo. Evaluar la transmisión transovárica del virus del dengue en larvas y pupas recolectadas en áreas rurales del municipio de Anapoima.Materiales y métodos. Se recolectaron ejemplares vivos en 53 viviendas y se transportaron al laboratorio de Anapoima, donde se clasificaron, se agruparon y se congelaron. Llevadas a Bogotá, se las homogeneizó, se les extrajo el ARN con Trizol®, se las sometió a una reacción en cadena de la polimerasa de transcripción inversa (Reverse Transcription Polymerase Change Reaction, RT-PCR) y a PCR convencional, y los productos amplificados se analizaron en geles de agarosa al 2 %.Resultados. En 54,7 % de las viviendas evaluadas se encontraron formas inmaduras del vector y el serotipo más frecuente fue el DENV-1. Sin embargo, en algunos pools se detectó la presencia simultánea de los serotipos DENV 1 y 2, DENV 1 y 3, y DENV 1 y 4, así como los serotipos DENV 1, 2 y 3.Conclusión. Los resultados confirmaron la transmisión vertical del virus de manera natural en el área rural del municipio, lo cual reafirma la capacidad vectorial de A. aegypti y explica, en parte, la persistencia del virus en la región y la posibilidad de que en la fase adulta el vector lo transmita sin haber consumido sangre infectada. Esta situación aumenta el riesgo de infección por el virus del dengue en Colombia y, por lo tanto, la necesidad de adelantar programas de prevención y control en todas las zonas con presencia del mosquito.

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Natural vertical transmission of dengue viruses in Aedes aegypt in southern India

Cited by 39 publications

References 1 publication

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

Household survey of container–breeding mosquitoes and climatic factors influencing the prevalence of Aedes aegypti (Diptera: Culicidae) in Makkah City, Saudi Arabia

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

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