Natural Sources and Applications of Demineralized Bone Matrix in the Field of Bone and Cartilage Tissue Engineering

Cho, Hunhwi; Bucciarelli, Alessio; Kim, Wonkyung; Jeong, Yongwoon; Kim, Namyeong; Jung, Junjae; Yoon, Sang-Hoon; Khang, Gilson

doi:10.1007/978-981-15-3258-0_1

Cited by 19 publications

(17 citation statements)

References 124 publications

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“…However, when only cells are transplanted, a number of apoptosis occurs for insufficient matrix support and the activation of the inflammatory system. For this reason implanting the cell on a proper scaffold that support their growth acting as extracellular matrix (ECM) is a valid alternative for an effective treatment of the cartilage and has been reported to be more effective than the sole use of cell transplantation (Cho et al., 2020; Man et al., 2016). Scaffolds used as cell carriers are one of the major accomplishment of tissue engineering and they are studied in a wide range of applications (skin, bone, cartilage, and for several organs), and they resulted to be essential for healing large cartilage defects (K. M. Park et al., 2009; Seal et al., 2001).…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Been

Choi

Cho

et al. 2021

J Tissue Eng Regen Med

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Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low‐cell density. The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self‐healed in several weeks, but major injuries may eventually require surgery. In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time. In recent years, cell therapy, in which cells are directly transplanted, has attracted attention. In this study, a scaffold for implanting chondrocytes was prepared. The scaffold was made as a sponge using the eggshell membrane and agarose. The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability. However, scaffolds made of collagen only has poor mechanical properties. For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water‐soluble, and then mixed with agarose to prepare a scaffold. Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three‐dimensional porosity. The scaffold was examined for Fourier‐transform infrared, mechanical properties, biodegradability, and biocompatibility. In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated. The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Been

Choi

Cho

et al. 2021

J Tissue Eng Regen Med

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“…It can be used as a scaffold for the growth of new bone tissue and has the characteristics of bone conduction and bone induction to promote bone regeneration (13). Due to the large supply on the market, the use of demineralized bone matrix for bone treatment is relatively easy (14). Yanaga et al (15) have confirmed that autologous chondrocytes can repair craniofacial or nasal deformities.…”

Section: Discussionmentioning

confidence: 99%

Study on specific proteins involved in articular cartilage regeneration activity induced by decalcified bone transplantation

Wang¹,

Dai²,

Feng³

et al. 2021

Ann Transl Med

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Background: Through a comprehensive analysis of the joint synovial fluid produced in the process of rabbit articular cartilage regeneration, the role and characteristics of knee synovial fluid in the process of decalcified bone transplantation-induced articular cartilage regeneration were explored.Methods: Twenty New Zealand white rabbits (approximately 2.5 kg in weight) were selected, and bilateral distal femoral bones from two randomly selected rabbits were extracted. After decalcification, the bones were cut into 2 mm × 4 cm long decalcified bone strips. Meanwhile, the other 18 rabbits were randomly divided into three groups: the test group (8 rabbits), the positive control group (6 rabbits), and the blank group (4 rabbits). In the test group, the decalcified bone joint was transplanted into the rabbits at the articular cartilage defect; in the positive control group, the articular cartilage defect of the rabbits were treated and put aside; in the blank group, no rabbits were treated. On the day of transplantation, and on the 4th, 8th, 12th, and 16th weeks after transplantation, the joint synovial fluid of each group was taken for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) analysis, and related database verification and identification, and compared with the positive control group and the blank group.Results: Using 2D-PAGE to separate various proteins in the synovial fluid of the rabbit knee joints, it was found that there were differential protein spots in the test group compared with the blank group and the positive control group. After conducting a comparative search and query in the UniProt database, through comprehensive analysis, it was finally found that three proteins with molecular weights of 23,429.4, 57,431.4, and 26,071.1 that may be related to the regeneration of articular cartilage appeared in the test group. Conclusions:In the process of inducing the regeneration of articular cartilage using decalcified bone transplantation, knee joint synovial fluid produced specific proteins, which may play an important role in the regeneration of articular cartilage. These findings may offer novel ideas in laying a foundation for the indepth study of articular cartilage regeneration.

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“…Therefore, a lot of effort will be needed to identify unknown effects of DBM. [ 63 ] In this study, the combination of nano‐DBM and PCL/β‐TCP scaffolds was an attempt to fabricate new material for bone defect treatment. The presence of nano‐DBM as coating material could increase new bone tissue growth ability of PCL/β‐TCP scaffolds.…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Study of a Novel Nanoscale Demineralized Bone Matrix Coated PCL/β‐TCP Scaffold for Bone Regeneration

Yuan

Wang

Zhao

et al. 2020

Macromolecular Bioscience

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Bone defects remains a challenge for surgeons. Bone graft scaffold can fill the defect and enhance the bone regeneration. Demineralized bone matrix (DBM) is an allogeneic bone graft substitute, which can only be used as a filling material rather than a structural bone graft. Coating of the scaffolds with nanoscale DBM may enhance the osteoinductivity or osteoconductivity. Herein the lyophilization method is presented to coat the nano‐DBM on surface of the porous polycaprolactone (PCL)/β‐tricalcium phosphate (β‐TCP) scaffolds fabricated by 3D printing technology. The morphology, elastic modulus, in vitro cell biocompatibility, and in vivo performance are investigated. Scanning electron microscope (SEM) shows DBM particle clusters with size of 200–500 nm are observed on scaffolds fibers after coating. MC3T3‐E1 cells on nano‐DBM coated PCL/β‐TCP scaffold show better activity than on PCL/β‐TCP scaffold. In vivo tests show better infiltration of new bone tissue in nano‐DBM coated PCL/β‐TCP scaffold than PCL/β‐TCP scaffold via the interface. These results show the presence of nano‐DBM coating on PCL/β‐TCP scaffold could enhance the attachment, proliferation, and viability of cells and benefit for the new bone formation surrounding and deep inside the scaffolds. Nano‐DBM could potentially be used as a new kind of biomaterial for bone defect treatment.

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Natural Sources and Applications of Demineralized Bone Matrix in the Field of Bone and Cartilage Tissue Engineering

Cited by 19 publications

References 124 publications

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration

Study on specific proteins involved in articular cartilage regeneration activity induced by decalcified bone transplantation

In Vitro and In Vivo Study of a Novel Nanoscale Demineralized Bone Matrix Coated PCL/β‐TCP Scaffold for Bone Regeneration

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