Natural Products Produced in Culture by Biosynthetically Talented Salinispora arenicola Strains Isolated from Northeastern and South Pacific Marine Sediments

Abstract

Laboratory cultures of two ‘biosynthetically talented’ bacterial strains harvested from tropical and temperate Pacific Ocean sediment habitats were examined for the production of new natural products. Cultures of the tropical Salinispora arenicola strain RJA3005, harvested from a PNG marine sediment, produced salinorcinol (3) and salinacetamide (4), which had previously been reported as products of engineered and mutated strains of Amycolatopsis mediterranei, but had not been found before as natural products. An S. arenicola strain RJA4486, harvested from marine sediment collected in the temperate ocean waters off British Columbia, produced the new aminoquinone polyketide salinisporamine (5). Natural products 3, 4, and 5 are putative shunt products of the widely distributed rifamycin biosynthetic pathway.

Marine natural products

“…Salinispora arenicola yielded a new aminoquinone polyketide salinisporamine 34 , and two lactones salinorcinol 35 and salinacetamide 36 . 15 The structure of proton-deficient 34 could not be assigned by NMR spectroscopy alone and elucidation was aided by XRD analysis. The structures of 35 and 36 have previously been reported from a mutated rifamycin producer A. mediterranei but never as NPs from a wild-type bacterial culture.…”