Natural Products as Source of Potential Dengue Antivirals

Teixeira, Róbson Ricardo; Pereira, Wagner Luiz; Oliveira, Ana Flávia Costa da Silveira; Silva, Antônio Alberto da; Oliveira, André Silva de; Silva, Milene Lopes da; Silva, Cynthia Cânedo da; Paula, Sérgio Oliveira de

doi:10.3390/molecules19068151

Cited by 62 publications

(49 citation statements)

References 63 publications

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“…For centuries, plants have acted as sources of compounds with biological properties, among which are included antiviral effects against viruses such as DENV [24] and CHIKV [25], demonstrating their ability to inhibit some of the viral replication cycle processes in the cell (from entry to the release of new viruses). In this context, our working group recently showed that extracts obtained from plants in the Colombian Caribbean region significantly inhibit DENV infection in cell culture [26].…”

Section: Introductionmentioning

confidence: 99%

Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections

Gómez-Calderón

Mesa-Castro

Robledo

et al. 2017

BMC Complement Altern Med

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BackgroundThe transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells.MethodsDry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 μg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis.ResultsThe compounds showed low toxicity at concentrations ≤ 200 μg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01).ConclusionIn vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.

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Section: Introductionmentioning

confidence: 99%

Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections

Gómez-Calderón

Mesa-Castro

Robledo

et al. 2017

BMC Complement Altern Med

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“…In this respect, natural products continue to play a critical role in drug discovery, since a significant portion (28%) of new drugs approved between 1981 and 2010 were of natural product origin [Newman and Cragg, ]. Natural products derived from plants have been successfully used to treat a variety of vector ailments, such as malaria, and have potential for the discovery of novel antiviral agents [Teixeira et al, ; Newman and Cragg, ].…”

Section: Introductionmentioning

confidence: 99%

Identification of resveratrol analogs as potent anti‐dengue agents using a cell‐based assay

Han

Penthala

Oliveira

et al. 2016

Journal of Medical Virology

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Dengue virus (DENV) causes a variety of difficult-to-treat diseases that threaten almost half of the world's population. Currently, no effective vaccine or antiviral therapy is available. We have examined a series of synthetic resveratrol analogs to identify potential anti-DENV agents. Here, we demonstrate that two resveratrol analogs, PNR-4-44 and PNR-5-02, possess potent anti-DENV activity with EC values in the low nanomolar range. These two resveratrol analogs were shown to mainly target viral RNA translation and viral replication, but PNR-5-02 is also likely to target cellular factors inside host cells. Although the precise molecular mechanism(s) mediating anti-DENV activities have not been elucidated, further structure-guided design might lead to the development of newer improved resveratrol derivatives that might have therapeutic value. J. Med. Virol. 89:397-407, 2017. © 2016 Wiley Periodicals, Inc.

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“…Because of their few (or unknown) adverse effects, the global use of medicinal plants or herbal-based medicines is steadily growing. Even though a number of plants are known for their anti-DENV and anti-CHIKV activities, few investigations have been published on the isolation (identification) and evaluation of compounds from plants with anti-DENV and anti-CHIKV activities [16].…”

Section: Natural Products As a Source Of Pharmaceuticalsmentioning

confidence: 99%

“…According to a WHO factsheet, 80% of the population of certain Asian and African countries depend on traditional medicines for primary health care due to economic and geographic constraints [16,[22][23][24][25]. Because of their few (or unknown) adverse effects, the global use of medicinal plants or herbal-based medicines is steadily growing.…”

Section: Natural Products As a Source Of Pharmaceuticalsmentioning

confidence: 99%

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Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses

Oliveira

Teixeira

Oliveira

et al. 2017

Preprint

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Abstract:Dengue virus (DENV) and chikungunya virus (CHIKV) are reemergent arboviruses that are transmitted by mosquitoes of the Aedes genus. During the last several decades, these viruses have been responsible for millions of cases of infection and thousands of deaths worldwide. Therefore, several investigations were conducted over the past few years to find antiviral compounds for the treatment of DENV and CHIKV infections. One attractive strategy is the screening of compounds that target enzymes involved in the replication of both DENV and CHIKV. In this review, we describe advances in the evaluation of natural products targeting the enzymes involved in the replication of these viruses.

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Natural Products as Source of Potential Dengue Antivirals

Cited by 62 publications

References 63 publications

Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections

Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections

Identification of resveratrol analogs as potent anti‐dengue agents using a cell‐based assay

Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses

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