Natural Polymeric Beads for Encapsulation of Stellaria media Extract with Antioxidant Properties

Abstract

Natural polymers are biodegradable and biocompatible and can be used as carriers of pharmaceutical active compounds, with good results for the enhancement of their stability characteristics.Plant extracts of the Cariophyllaceae family are of great interest due to antioxidant, anti-inflammatory, carminative, anticancer, antibacterial, antifungal, antiviral properties, and more recently, the ability to inhibit intestinal carboxylation and reduce obesity by complex mechanisms.The aim of our study was tocharacterize the Stellaria media aqueous extract from the point of view of total polyphenols (using Folin-Ciocalteu method) and antioxidant capacity, as well as the efficiency of inclusion of Stellaria extract in calcium chloride alginate beads by two different methods. In the same time, the size of alginate microcapsules was investigated along with the released efficiencyfrom the polymeric matrix in different biological fluids.Two different methods for encapsulation of Stellaria media extract were developed in this work, using calcium alginate beads. Encapsulating efficiency shows a better approach within the method in which the extract was in situ encapsulated in �pearls�-shaped beads.

Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

“…The bioactive compounds and the antioxidant capacity of Stellaria media (L.) Vill. in this study are comparable with the results obtained by the same authors Miere (Groza) et al, 2019 [17] on the same plant, but harvested from another area (around the Crisul Repede river, Oradea city, Bihor county, Romania).…”

“…The antioxidant capacity of Stellaria media (L.) Vill. extract was determined using the DPPH method, as previously described in the literature [17,19,20]. DPPH radical reduction was monitored spectrophotometrically at 517 nm using the Shimadzu mini UV-VIS spectrophotometer.…”

“…2.1.1. Total phenols content using the Folin-Ciocalteu method The total phenols content was determined using the Folin-Ciocalteu method according to the literature [17,18]. Lyophilized extract of the plant Stellaria media (L.) Vill.…”

“…The determination of EE% was performed according to the protocol described by Gibis et al, 2016 [24], with some modifications. Thus, the inclusion efficiency was calculated while taking into account the content of total phenols in the extract, which was determined using the Folin-Ciocâlteu method [17,19], after destroying the liposomal membranes with Triton X-100 0.5% (v/v) in order to release extract.…”

Electrochemical Methods for Evaluation of Antioxidant Properties of Propolis Extract Incorporated in Chitosan Nanoparticles

“…Both spectrophotometric and electrochemical assays can be used in order to evaluate the antioxidant capacity of natural biological compounds, including the propolis extract. Spectrophotometric assays are based on the redox reaction with an oxidant: TEAC (Trolox Equivalent capacity, FRAP (ferric ion reducing antioxidant parameter), DPPH ((2,2-diphenyl-1-picryl-hydrazyl-hydrate), CUPRAC (Cu(II) reduction capacity) and Folin-Ciocalteu assay for total phenolics determination [15][16][17][18][19][20][21]. The main drawbacks of these methods are related to the high cost of reactants and different interferences with other colorful compounds in the sample.…”