Electrochemical Methods for Evaluation of Antioxidant Properties of Propolis Extract Incorporated in Chitosan Nanoparticles

Fritea, Luminița; Pasca, Paula Melania; Vlase, Laurian; Gheldiu, Ana-Maria; Moldovan, Liviu; Bănică, Florin; Dobjanschi, Luciana; Cavalu, Simona

doi:10.37358/mp.20.4.5410

Cited by 7 publications

(7 citation statements)

References 39 publications

(72 reference statements)

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“…Determination of the surface electric charge or Zeta potential is important because it indicates the stability of the liposomal emulsion [22,23]. The tests were performed for each type of obtained liposome (PC, PS, PCE and PSE) using a disposable folded capillary cell.…”

Section: Average Diameter and Zeta Potential Measurements By Dlsmentioning

confidence: 99%

Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

et al. 2021

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Liposomes continue to attract great interest due to their increased bioavailability in the body and because the substances encapsulated are protected while maintaining their effectiveness. The aim of this study is to obtain “giant” liposomes by lipid film hydration using a preparation formula with two different phospholipids, phosphatidylcholine (PC) and phosphatidylserine (PS). Firstly, the macro- and microscopic characterization, total phenols content and antioxidant capacity of the plant Stellaria media (L.) Vill. were assessed. Then, Stellaria media (L.) Vill. extract was encapsulated in both formulations (PCE and PSE) and the liposomes were characterized according to their morphology, size distribution and Zeta potential using optical microscopy and dynamic light scattering. The encapsulation efficiency (EE%) was determined using the Folin–Ciocalteu method and the values of both formulations were compared. PC and PCE liposomes with a diameter between 712 and 1000 nm and PS and PSE liposomes with a diameter between 58 and 1000 nm were obtained. The values EE% of Stellaria media (L.) Vill. extract for PCE and PSE were 92.09% and 84.25%, respectively.

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Section: Average Diameter and Zeta Potential Measurements By Dlsmentioning

confidence: 99%

Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

et al. 2021

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“…The peaks revealed by the DPV (Figure 2) can be assigned to the oxidation of the electroactive phenolic species from the extract [8,21,[54][55][56]. The peak, which appeared at around 0.450 V, is due to the presence of ascorbic acid from the extract composition, as it was also revealed by other authors in comparable amounts [57].…”

Section: Discussionmentioning

confidence: 55%

Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

et al. 2021

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The healing of skin wounds remains an important concern in medicine, especially in chronic wounds caused by various diseases such as diabetes. Using herbs or herbal products to heal skin wounds is a therapeutic challenge for traditional medicine. In this context, the main aim of our work was to highlight the in vitro healing potential of Stellaria media (L.) Vill. (SM) extract using the scratch assay on normal human dermal fibroblasts (NHDF). The ability to stimulate cell migration and proliferation under the influence of different concentrations of SM extract (range between 12.5 and 200 µg/mL) was determined compared to the control (untreated in vitro-simulated wound) and positive control (allantoin 50 µg/mL). Our results showed that the concentration of 100 µg/mL SM extract applied on the simulated wound recorded the strongest and fastest (24 h) migration (with wound closure) and proliferation of NHDF compared with the control. In addition, the SM extract was characterized in terms of bioactive compounds (total phenols and flavonoids content), antioxidant capacity (FRAP (The Ferric-Reducing Antioxidant Power) assay and electrochemical method), and antimicrobial activity. The results show that the SM extract contains a considerable amount of polyphenols (17.19 ± 1.32 mg GAE/g dw and 7.28 ± 1.18 mg QE/g dw for total phenol and flavonoid content, respectively) with antioxidant capacity. Antimicrobial activity against Gram-positive bacteria (S. aureus) is higher than E. coli at a dose of 15 µg/mL. This study showed that Stellaria media is a source of polyphenols compounds with antioxidant capacity, and for the first time, its wound healing potential was emphasized.

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“…Electrochemical biosensors based on enzymes (tyrosinase, laccase, peroxidase) and DNA have been developed for determining the antioxidant activity of natural compounds, using different techniques such as cyclic voltammetry (CV), square-wave voltammetry (SWV), and differential pulse voltammetry (DPV) [19,20]. Other electrochemical sensors (without biorecognition element) have been applied for the detection of non-enzymatic antioxidants (especially polyphenols), many of them being modified with nanomaterials [19,21,22]. The electrochemical behavior of the mixt extract OB+TP analyzed by DPV is presented in Figure 1a indicating two oxidation peaks at around 0.250 V and 0.900 V, respectively.…”

Section: Antioxidant Capacity Of Ob+tp Extract Mixture Evaluated By E...mentioning

confidence: 99%

“…The mixture OB+TP presented a calculated AEAC of 17.36 mg/g with an RSD of 2.05%. The two anodic peaks recorded by the DPV technique can be attributed to the oxidation process of the catechol groups from polyphenols and flavonoids existing in considerable amounts in plant extracts [6,21,22]. The high values for the ΔEp obtained by the CV technique indicate an irreversible redox process of the constituents.…”

Section: Antioxidant Capacity Of Ob+tp Extract Mixture Evaluated By E...mentioning

confidence: 99%

Novel Topical Formulations Based on O. Basilicum and T. Pratense: Antioxidant, Antimicrobial, and Anti-Inflammatory Effect

Antonescu¹,

Antonescu²,

Miere³

et al. 2022

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This study aimed to highlight the importance of phytochemical-based formulations in the treatment of wounds with different etiologies, avoiding the possible side effects generated by conventional treatments. We present the preparation method of novel topical formulations (cream, ointment, and hydrogel) based on mixed extracts of Ocimum basilicum and Tifolium pratense along with evidence of their antimicrobial and antioxidant effect in vitro. The anti-inflammatory and wound healing properties of the new formulations were evaluated against different chronic inflammatory processes by presenting three clinical cases of chronic skin diseases. The clinical cases presented in our study revealed good outcomes of the cream formulation in the case of dyshidrotic eczema, ointment formulation in the case of hypertrophic scar, and hydrogel formulation in the case of psoriasis lesions. The best effect was noticed after 6 days of topical application of hydrogel on psoriatic plaques, resulting in a significant reduction of erythematous plaques.

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Electrochemical Methods for Evaluation of Antioxidant Properties of Propolis Extract Incorporated in Chitosan Nanoparticles

Cited by 7 publications

References 39 publications

Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

Novel Topical Formulations Based on O. Basilicum and T. Pratense: Antioxidant, Antimicrobial, and Anti-Inflammatory Effect

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