Natural Peste des Petits Ruminants Virus Infection: Novel Pathologic Findings Resembling Other Morbillivirus Infections

Abstract

Abstract. The present study describes pathologic and virologic findings in 15 sheep and 6 goats that died of natural peste des petits ruminants virus infection in Turkey. Pathologic findings included erosiveulcerative stomatitis, fibrino-necrotic tracheitis, bronchointerstitial pneumonia, multifocal coagulation necroses in the liver, and severe lymphocytolysis in lymphoid tissues. Syncytial cells were conspicuous, especially in the oral mucosa, pulmonary alveoli, liver, and lymphoid tissues. In addition to the typical tissue distribution, eosinophilic intracytoplasmic and/or intranuclear inclusions were observed in epithelial cells lining the renal pelvis and abomasal mucosa. Immunolabeling of the viral antigen was observed in the kidney, brain, rumen, abomasum, heart, and myocytes of the tongue besides its more typical locations. In this study, we report and describe in detail the first peste des petits ruminants endemic in Kirikkale Province, Central Anatolia of Turkey. In conclusion, these previously unreported pathologic findings in natural peste des petits ruminants virus infection establish a basis for resemblance to other morbillivirus infections, such as canine distemper and distemper of sea mammals. Reverse transcriptase-polymerase chain reaction analyses indicated that the 448-bp genome fragment was amplified in 18 cases (18/21, 85.7 %). Phylogenetic analysis showed that viruses belong to lineage 4 in the peste des petits ruminants virus common phylogenetic tree.

Pathomorphological Studies of Peste Des Petits Ruminants (PPR) in Goats of Navsari and Valsad Districts, India

“…Histopathological examination of lungs revealed alveolar dilatation, emphysema and infiltration of mononuclear cells along with pink colour serous exudate which was shown similarity with the earlier observations of various researchers (Aktas et al, 2011;Jagtap et al, 2012;Patel et al, 2015 andMaina et al, 2015). Presence of syncytial cells and eosinophilic intracytoplasmic inclusion body in bronchial epithelium cells was supported by the earlier reports of Brown et al, (1991), Islam et al, (2001), Kumar et al, (2004), Toplu (2004), Kul et al, (2007) and Saglam and Temur (2009). Though interstitial pneumonia was evident and partially agreement with the findings of Kumar et al, (2004) and Kul et al, (2007).…”

“…Presence of syncytial cells and eosinophilic intracytoplasmic inclusion body in bronchial epithelium cells was supported by the earlier reports of Brown et al, (1991), Islam et al, (2001), Kumar et al, (2004), Toplu (2004), Kul et al, (2007) and Saglam and Temur (2009). Though interstitial pneumonia was evident and partially agreement with the findings of Kumar et al, (2004) and Kul et al, (2007). Presence of exudate in bronchi and hyperplasia of brochial epithelium was noted in present study which was also same as the findings of Aktas et al, (2011) and Maina et al, (2015).…”

“…According to present and earlier reports of Islam et al, (2001), Kul et al, (2007), Maina et al, (2015) and Patel et al, (2015) lymphoid cell depletion was observed in cortex and medullary cords of spleen and lymph node. It might be due to the sever lymphocytolysis in lymphoid tissues by PPR virus (Kul et al, 2007).…”

Therapeutic response of West African Dwarf goats infected with Peste des Petits Ruminants whose oral lesions were treated with oxytetracycline long acting and gentian violet topical spray

“…The first report of PPR was in the Cote d'Ivoire, but it has been reported in other parts of the world like in sub-Saharan region, Middle East and other parts of Asian continent [5][6][7]. PPR virus (PPRV), the aetiologic agent of this devastating animal viral disease is a morbillivirus of the family Paramyxoviridae [8].…”

“…The oral lesions in PPR clinical infection are characterized by swelling around the mouth, erosion, ulceration and necrosis on the lips, gums, buccal cavity, tongue and palate [6,16]. These oral lesions are subsequently The oral lesions in one group were cleaned with 70% alcohol and treated with oxytetracycline long acting (LA) intramuscularly (IM) and topically treated with gentian violet spray which also contained oxytetracycline, and in another group WAD goats infected with PPR virus were only treated with oxytetracycline LA intramuscularly.…”