Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo

Wu, Peng; Song, Ziteng; Li, Ying; Wang, Huimei; Zhang, Han; Bao, Jiahe; Li, Yuhao; Cui, Jianlin; Jin, Da Qing; Wang, Aijie; Liang, Bin; Lee, Dong‐Ho; Xu, Jing; Guo, Yuanqiang

doi:10.1016/j.bioorg.2020.104331

Cited by 11 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iridoids are an important type of chemical constituents of the genus Patrinia . Previous investigations showed that some iridoids from P. heterophylla and P. scabra possessed significant anti-inflammatory activity [ 35 , 36 , 37 ]. However, studies on the iridoids in P. scabiosifolia were mainly focused on their cytotoxic and AChE inhibitory activities, and studies on the anti-inflammatory activity of the iridoids from this plant are still insufficient [ 22 , 25 ].…”

Section: Resultsmentioning

confidence: 99%

Two New Iridoid Glucosides from the Whole Plant of Patrinia scabiosifolia Link

Sun

Liu

et al. 2021

Molecules

View full text Add to dashboard Cite

As a traditional Chinese medicine, Patrinia scabiosifolia Link has been used to treat various inflammatory-related diseases, and recent studies have shown that it possesses potent anti-inflammatory activity. Therefore, phytochemical investigation on whole plants of P. scabiosifolia were carried out, which led to the isolation of two new iridoid glucosides, patriniscabiosides A (1) and B (2), together with six known compounds (3–8). The structural elucidation of all compounds was performed by HRESIMS and extensive spectroscopic analyses including IR, 1D, 2D NMR, and electronic circular dichroism (ECD). All the isolated compounds were tested for their anti-inflammatory activity using the NF-κB-Dependent Reporter Gene Expression Assay, and compound 3 displayed anti-inflammatory activity through the inhibition of the NF-κB pathway, with an inhibitory rate of 73.44% at a concentration of 10 μM.

show abstract

Section: Resultsmentioning

confidence: 99%

Two New Iridoid Glucosides from the Whole Plant of Patrinia scabiosifolia Link

Sun

Liu

et al. 2021

Molecules

View full text Add to dashboard Cite

show abstract

“…[27] Additionally, NO can upregulate the expression of COX-2 through NO/c-GMP pathway. [28][29] Both proteins participate in the occurrence and development of inflammation and are essential for the NO formation in the inflammatory process. To verify the conjecture, molecular docking studies were performed to investigate the binding abilities and sites of the active compound 11 with proteins iNOS and COX-2.…”

Section: Resultsmentioning

confidence: 99%

Meroterpenoids Possessing Diverse Rearranged Skeletons with Anti‐inflammatory Activity from the Mangrove‐Derived Fungus Penicillium sp. HLLG‐122

Qin

Zhang

et al. 2023

Chin. J. Chem.

View full text Add to dashboard Cite

Comprehensive SummaryFifteen new meroterpenoids, littoreanoids A—O (1—15), including three rearranged skeleton meroterpenoids (1—3), were isolated from the mangrove‐derived fungus Penicillium sp. HLLG‐122. Compound 1 was a novel berkeleyacetal‐derived meroterpenoid featuring an unusual spirocyclic 2‐oxaspiro[5.5]undeca‐4,7‐dien‐3‐one moiety. Compound 2 possessed an unusual 6/6/6/6/6 pentacyclic system with a novel 1‐hydroxy‐7,7‐dimethyl‐2‐oxabicyclo[2.2.2]octan‐5‐yl acetate moiety. Compound 3 was an unusual 6/7/6/5/6/5/4 polycyclic systems containing a β‐lactone ring. The structures and absolute configurations of new compounds were elucidated by HRESIMS, NMR spectroscopy, single crystal X‐ray diffraction analysis, and electronic circular dichroism calculations. The plausible biosynthetic pathways of 1—3 were also proposed. Compounds 6 and 11 exhibited anti‐inflammatory effects with IC50 values of 30.41 and 19.02 μmol/L, respectively. The bioactive compound 11 was selected for the investigation of preliminary mechanism using molecular docking and Western blotting experiments. Compound 11 could suppress the levels of TNF‐α and IL‐6, and down‐regulate the protein expression of iNOS and COX‐2 in RAW 264.7 cells.

show abstract

“…The other herbal plants that belong to the same category of P. scabra have been studied on the analgesic and anti-inflammatory activities. For example, the iridoids of Patrinia heterophylla was reported to have the anti-inflammatory effects, which could inhibit NO activities and reduce the ROS production through vitro and vivo experiments [ 45 ]. Patrinia villosa was found to show strong anti-inflammatory and anti-nociceptive effects through a series of animal experiments including the ear edema test, carrageenan-induced paw edema test, cotton pellet-induced granuloma formation test, and acetic acid-induced writhing test.…”

Section: Discussionmentioning

confidence: 99%

Optimization for ultrasonic-microwave synergetic extraction of total iridoid glycosides and screening of analgesic and anti-inflammatory active fractions from patrinia scabra Bunge (Valerianaceae)

Deng

et al. 2022

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Patrinia scabra Bunge is a well-known herbal medicine for its favorable treatment on inflammatory diseases owing to its effective ingredients, in which iridoid glycoside plays an extremely significant role. This article aimed to improve the content of total iridoid glycosides in crude extract through a series optimization of extraction procedure. Moreover, considering that both pain and inflammation are two correlated responses triggered in response to injury, irritants or pathogen, the article investigated the anti-inflammatory and analgesic activities of P. scabra to screen out the active fraction. Method P. scabra was extracted by ultrasonic-microwave synergistic extraction (UMSE) to obtain total iridoid glycosides (PSI), during which a series of conditions were investigated based on single-factor experiments. The extraction process was further optimized by a reliable statistical method of response surface methodology (RSM). The elution fractions of P. scabra extract were prepared by macroporous resin column chromatography. Through the various animal experiment including acetic acid-induced writhing test, formalin induced licking and flinching, carrageenan-induced mice paw oedema test and xylene-induced ear edema in mice, the active fractions with favorable analgesic and anti-inflammatory effect were reasonably screen out. Results The content of PSI could reach up to 81.42 ± 0.31 mg/g under the optimum conditions as follows: ethanol concentration of 52%, material-to-liquid ratio of 1:18 g/mL, microwave power at 610 W and extraction time of 45 min. After gradient elution by the macroporous resin, the content of PSI increased significantly. Compared with other concentrations of elution liquid, the content of PSI in 30 and 50% ethanol eluate was increased to reach 497.65 and 506.90 mg/g, respectively. Owing to the pharmacology experiment, it was reasonably revealed that 30 and 50% ethanol elution fractions of P. scabra could relieve pain centrally and peripherally, exhibiting good analgesic and anti-inflammatory activities. Conclusion Patrinia scabra possessed rich iridoids and exhibited significant analgesic and anti-inflammatory activities.

show abstract

Natural iridoids from Patrinia heterophylla showing anti-inflammatory activities in vitro and in vivo

Cited by 11 publications

References 46 publications

Two New Iridoid Glucosides from the Whole Plant of Patrinia scabiosifolia Link

Two New Iridoid Glucosides from the Whole Plant of Patrinia scabiosifolia Link

Meroterpenoids Possessing Diverse Rearranged Skeletons with Anti‐inflammatory Activity from the Mangrove‐Derived Fungus Penicillium sp. HLLG‐122

Optimization for ultrasonic-microwave synergetic extraction of total iridoid glycosides and screening of analgesic and anti-inflammatory active fractions from patrinia scabra Bunge (Valerianaceae)

Contact Info

Product

Resources

About