Natural Inhibitors of DNA Topoisomerase I with Cytotoxicities

Han, Hui; Tan, Ning‐Hua; Zeng, Guang‐Zhi; Fan, Junting; Huang, Hai; Ji, Chang‐Jiu; Jia, Rui-Rui; Zhao, Qin‐Shi; Zhang, Yingjun; Hao, Xiao‐Jiang; Wang, Liqin

doi:10.1002/cbdv.200890124

Cited by 27 publications

(22 citation statements)

References 13 publications

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“…Compounds 1-6 were tested for anti-HBV activity in HBV antigen secretion and DNA replication of HepG 2.2.15 cells using the literature methods [20][21][22] …”

Section: Resultsmentioning

confidence: 99%

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Astataricusones A–D and Astataricusol A, Five New Anti-HBV Shionane-Type Triterpenes from Aster tataricus L. f.

Zhou

Zeng

et al. 2013

Molecules

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Abstract:Five new shionane-type triterpenes, astataricusones A-D (compounds 1-4) and astataricusol A (5), together with one known shionane-type triterpene 6 were obtained from the roots and rhizomes of Aster tataricus L. f. Their structures were elucidated on the basis of spectroscopic data, mainly NMR and MS data. The absolute configurations of 1 and 4 was determined by single crystal X-ray diffraction and CD analysis. Compound 2 showed inhibitory activity on HBsAg secretion with an IC 50 value of 23.5 μM, while 2 and 6 showed inhibitory activities on HBeAg secretion with IC 50 values of 18.6 and 40.5 μM, and cytotoxicity on HepG 2.2.15 cells with CC 50 values of 172.4 and 137.7 μM, respectively. Compounds 2 and 6 also exhibited inhibitory activities on HBV DNA replication with IC 50 values of 2.7 and 30.7 μM, respectively.

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“…Compounds 1-6 were tested for anti-HBV activity in HBV antigen secretion and DNA replication of HepG 2.2.15 cells using the literature methods [20][21][22] …”

Section: Resultsmentioning

confidence: 99%

“…Cytotoxicity of compounds 1-6 on HepG 2.2.15 cells was tested by a sulforhodamine B method [22] (SRB, Sigma, St. Louis, MO, USA). Firstly, HepG 2.2.15 cells were seeded in a 96-well microplate for 24 h. Compounds, dissolved in DMSO and diluted with the medium, were placed in each well and incubated for another 72 h at 37 °C.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Astataricusones A–D and Astataricusol A, Five New Anti-HBV Shionane-Type Triterpenes from Aster tataricus L. f.

Zhou

Zeng

et al. 2013

Molecules

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“…The procedures described by[1617] were followed. Briefly, the sulphorhodamine B (SRB) assay was adopted for a quant measurement of cell growth and viability.…”

Section: Methodsmentioning

confidence: 99%

Anticancer and antiradical scavenging activity ofAgeratum conyzoidesL. (Asteraceae)

et al. 2010

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Ageratum conyzoides has been used in folklore for the treatment of a wide range of diseases. In the present investigation, the in vitro activity of ethanol, petroleum ether, ethylacetate, butanol, and water extracts of A. conyzoides were screened in some cancer cell lines using the sulphorhodamine B (SRB) assay. These cell lines include: Human non-small cell lung carcinoma (A-549), human colon adenocarcinoma (HT-29), human gastric carcinoma (SGC-7901), human golima (U-251), human breast carcinoma (MDA-MB-231), human prostate carcinoma (DU-145), human hepatic carcinoma (BEL-7402), and mouse leukemia (P-388) cancer cell lines. Furthermore, kaempferol was isolated from the ethylacetate extract and the structure was elucidated by nuclear magnetic resonance (NMR) and mass spectroscopy. The effect of DPPH antiradical activity on the extracts and kaempferol was also determined. The results showed that ethylacetate extract exhibited the highest cytotoxic activity on A-549 and P-388 cancer cells with IC50 values of 0.68 and 0.0003 μg/ml, respectively. Kaempferol isolated from the ethylacetate extract of A. conyzoides rapidly scavenged DPPH at a concentration of 130.07 ± 17.36 g/kg. The result therefore showed that A. conyzoides possessed anticancer and antiradical properties.

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“…The sulphorhodamine B (SRB) assay has been adopted for a quant. measurement of cell growth and viability [32]. Cells were cultured in RPMI 1640 medium (Sigma).…”

Section: Experimental Partmentioning

confidence: 99%

Isolation and Characterization of Bioactive Cyclotides from Viola labridorica

Tang¹,

Wang²,

Pan³

et al. 2010

Helvetica Chimica Acta

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Many Violaceae plants contain cyclotides, which are plant cyclopeptides distinguished by a cyclic cystine knot motif with 28 -37 amino acid residues. In the current study, four new cyclotides, vila A -D (1 -4, resp.), together with a known cyclotide, varv D (5), were isolated from Viola labridorica (Violaceae). A chromatography-based method was used to isolate the cyclotides, which were characterized using tandem mass spectrometry and 2D-NMR spectroscopy. Several of the cyclotides showed cytotoxic activities against five cancer cell lines, i.e., U251, MDA-MB-231, A549, DU145, and BEL-7402, with vila A and B (1 and 2, resp.) being the most cytotoxic. The isolated cyclotides showed no antibacterial activity against Staphyloccocus aureus and Candida albicans. Homology modeling of the cyclotide structures was used to analyze structure -activity relationships.

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Natural Inhibitors of DNA Topoisomerase I with Cytotoxicities

Cited by 27 publications

References 13 publications

Astataricusones A–D and Astataricusol A, Five New Anti-HBV Shionane-Type Triterpenes from Aster tataricus L. f.

Astataricusones A–D and Astataricusol A, Five New Anti-HBV Shionane-Type Triterpenes from Aster tataricus L. f.

Anticancer and antiradical scavenging activity ofAgeratum conyzoidesL. (Asteraceae)

Isolation and Characterization of Bioactive Cyclotides from Viola labridorica

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