Natural infection of Nicandra physaloides by Tomato severe rugose virus in Brazil

Barbosa, J. C.; Barreto, Sarah S.; Inoue‐Nagata, Alice K.; Reis, Marcelo S.; Firmino, Ana Carolina; Filho, Armando Bergamin; Rezende, J. A. M.

doi:10.1007/s10327-009-0198-5

Cited by 40 publications

(39 citation statements)

References 14 publications

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“…; Barbosa et al. , ), they may serve as primary source of inoculum and vector. In the course of the experiments, leaf samples from Chenopodium album , C. ambrosioides , Nicandra physaloides and Sida urens naturally occurring near to the tomato fields were analysed by PCR and revealed as positive for ToSRV infection (data not shown).…”

Section: Discussionmentioning

confidence: 99%

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Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Barbosa

Rezende

Amorim

et al. 2015

Journal of Phytopathology

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Temporal progress of a begomovirus disease in tomato fields and the abundance of its whitefly vector, Bemisia tabaci biotype B, were evaluated during three consecutive tomato plantings in the municipality of Sumaré, state of São Paulo, Brazil, in 2006 and 2007. The incidence of symptomatic plants and the number of adult whiteflies were weekly monitored on experimental plots randomly chosen in tomato commercial fields. Tomato severe rugose virus (ToSRV) was identified as the causal agent of the disease, and its relationships with other Brazilian begomoviruses was confirmed by partial and complete nucleotide sequencing of the viral genome. The disease temporal progress was analysed by fitting different models to disease incidence. The monomolecular model showed the best fit, which is consistent with a predominant role of primary spread in the epidemiology of ToSRV. A higher number of adult whiteflies were observed at the borders of the plots, also suggesting primary spread of ToSRV from external sources of inoculum, which might be represented by weeds and volunteer tomato‐infected plants. In Brazil, since 2004, there is a legislative measure that mandates, for some regions of processing tomato plantings, a 2‐month crop‐free period during the year. Based on our results, we suggest the extension of this measure to all tomato‐producing regions, including fresh market tomato. We also suggest that growers emphasize the elimination of old plants from harvested fields that can serve as virus reservoirs several weeks prior to new plantings and weeds nearby the fields to limit the primary spread of ToSRV.

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Section: Discussionmentioning

confidence: 99%

“…d b 1 = estimative of maximum asymptote, b 2 = parameter related to initial inoculum and b 3 = disease progress rate. Barbosa et al 2009Barbosa et al , 2011Barreto et al 2013).…”

Section: Abundance Of Adult Whitefliesmentioning

confidence: 99%

Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Barbosa

Rezende

Amorim

et al. 2015

Journal of Phytopathology

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“…Several species of non-cultivated plants, especially of the families Malvaceae, Euphorbiaceae, Fabaceae and Solanaceae, are known hosts of begomoviruses (Morales & Anderson, 2001). These weed/wild hosts can serve as reservoirs for infection of nearby crops (Alabi et al, 2008;Barbosa et al, 2009;Bedford et al, 1998;García-Andrés et al, 2006), as overwintering refugia (Alabi et al, 2007(Alabi et al, , 2008García-Andrés et al, 2006) and as 'mixing vessels' for interspecific coinfection and recombination (García-Andrés et al, 2006;Monde et al, 2010;Silva et al, 2012). Increased host use and diminished bottlenecks would both potentially increase the effective population size of begomovirus populations (Power, 2000;Seal et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Synonymous site variation due to recombination explains higher genetic variability in begomovirus populations infecting non-cultivated hosts

Lima

Sobrinho

Aguilera

et al. 2013

Journal of General Virology

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Begomoviruses are ssDNA plant viruses that cause serious epidemics in economically important crops worldwide. Non-cultivated plants also harbour many begomoviruses, and it is believed that these hosts may act as reservoirs and as mixing vessels where recombination may occur. Begomoviruses are notoriously recombination-prone, and also display nucleotide substitution rates equivalent to those of RNA viruses. In Brazil, several indigenous begomoviruses have been described infecting tomatoes following the introduction of a novel biotype of the whitefly vector in the mid-1990s. More recently, a number of viruses from non-cultivated hosts have also been described. Previous work has suggested that viruses infecting non-cultivated hosts have a higher degree of genetic variability compared with crop-infecting viruses. We intensively sampled cultivated and non-cultivated plants in similarly sized geographical areas known to harbour either the weed-infecting Macroptilium yellow spot virus (MaYSV) or the crop-infecting Tomato severe rugose virus (ToSRV), and compared the molecular evolution and population genetics of these two distantly related begomoviruses. The results reinforce the assertion that infection of non-cultivated plant species leads to higher levels of standing genetic variability, and indicate that recombination, not adaptive selection, explains the higher begomovirus variability in non-cultivated hosts.

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“…According to this hypothesis, the source of the tomato viruses would be other unidentified (possibly symptomless) wild hosts. In this regard, it is noteworthy that Tomato severe rugose virus (ToSRV) has been reported to infect solanaceous weeds such as Nicandra physaloides in the field (Barbosa et al, 2009). Second, Sida spp.…”

Section: Discussionmentioning

confidence: 99%

Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp

Tavares

Ramos-Sobrinho

Aguilera³

et al. 2012

Planta daninha

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-Begomoviruses are whitefly-transmitted, single-stranded DNA viruses that are often associated with weed plants. The aim of this study was to further characterize the diversity of begomoviruses infecting weeds (mostly Sida spp.) in Brazil. Total DNA was extracted from weed samples collected in Viçosa (Minas Gerais state) and in some municipalities of Alagoas state in 2009 and 2010. Viral genomes were amplified by RCA, cloned and sequenced. A total of 26 DNA-A clones were obtained. Sequence analysis indicated the presence of 10 begomoviruses. All viral isolates from Blainvillea rhomboidea belonged to the same species, Blainvillea yellow spot virus (BlYSV ), thereby suggesting that BlYSV may be the only begomovirus present in this weed species. Four isolates represent new species, for which the following names are proposed: Sida yellow blotch virus (SiYBV), Sida yellow net virus (SiYNV), Sida mottle Alagoas virus (SiMoAV) and Sida yellow mosaic Alagoas virus (SiYMAV). Recombination events were detected among the SiYBV isolates and in the SiYNV isolate. These results constitute further evidence of the high species diversity of begomoviruses in Sida spp. However, the role of this weed species as a source of begomoviruses infecting crop plants remains to be determined.Keywords: geminivirus, recombination, Blainvillea rhomboidea, Sida micrantha, Sida urens, Sida santaremnensis RESUMO -Begomovírus são vírus de DNA circular fita simples transmitidos por mosca branca, os quais são frequentemente associados com plantas daninhas. O objetivo deste trabalho foi caracterizar a diversidade de begomovírus infectando plantas daninhas (principalmente Sida spp.) no Brasil. DNA total foi extraído a partir de plantas daninhas coletadas em Viçosa (Minas Gerais) e em alguns municípios do estado de Alagoas em 2009 e 2010. Os genomas virais foram amplificados por RCA, clonados e sequenciados. Um total de 26 clones de DNA-A foram obtidos. A análise das sequências indicou a presença de dez diferentes begomovírus. Todos os isolados originários de Blainvillea rhomboidea pertencem a uma única espécie viral, Blainvillea yellow spot virus (BlYSV), sugerindo que o BlYSV pode ser o único begomovírus presente nesta espécie de planta invasora. Quatro isolados representam espécies novas, para as quais os seguintes nomes são propostos: Sida yellow blotch virus (SiYBV), Sida yellow net virus (SiYNV), Sida mottle Alagoas virus (SiMoAV) e Sida yellow mosaic Alagoas virus (SiYMAV). Eventos de recombinação foram detectados entre isolados de SiYBV e no isolado de SiYNV. Estes resultados constituem uma evidência adicional da alta diversidade de espécies de begomovírus em Sida spp. Contudo, o possível papel dessas plantas daninhas como fonte de begomovírus para plantas cultivadas ainda permanece indeterminado.

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Natural infection of Nicandra physaloides by Tomato severe rugose virus in Brazil

Cited by 40 publications

References 14 publications

Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Temporal Dynamics of Tomato Severe Rugose Virus and Bemisia tabaci in Tomato Fields in São Paulo, Brazil

Synonymous site variation due to recombination explains higher genetic variability in begomovirus populations infecting non-cultivated hosts

Further molecular characterization of weed-associated begomoviruses in Brazil with an emphasis on Sida spp

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