Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters

El-Sheshtawy, Reda I.; El-Badry, Diya A.; El-Nattat, Walid S.; Almaaty, Amal M. Abo

doi:10.1016/j.apjr.2016.06.004

Cited by 26 publications

(21 citation statements)

References 15 publications

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“…Honey induces spermatogenesis (Abdul-Ghani et al, 2008) and one of its active ingredients is phenolic compound which has high antioxidant activity (Perez & Rodriguez-Malaver, 2006). Study using semen of Arab stallion by El-Sheshtawy et al (2016) found that semen supplemented with honey bee showed sperms protected from cryoprotectant damage and also suggested that this treatment was able to inhibit the disruption of the sperm DNA by free radicals induction. In addition, infertile semen supplemented with 10% of pure honey and cryoprotectant medium sustained the sperm motility about 50% after thawing compared to infertile semen treated with 5% pure honey.…”

Section: Discussionmentioning

confidence: 99%

“…At low temperature, honey does not freeze and the viscosity of honey increases with the decreased temperature. This occurrence creates a low surface tension that eventually minimizing the ice crystals formation inside the cytoplasm of sperm and reduce sperm damage during cryopreservation (Fuller, 2004;Fakhrildin et al, 2014;El-Sheshtawy et al, 2016). Moreover, honey contains high variety of simple sugars that might serve as a nutrition source and non-penetrating cryoprotectant to sperm cells during cryopreservation (Fuller, 2004).…”

Section: Discussionmentioning

confidence: 99%

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Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms

Maidin

Padlan

Azuan

et al. 2018

Trop. Anim. Sci. J.

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Some chemical compositions of polyphenol antioxidants found in Nigella sativa and honey have potential to protect sperm membrane from lipid peroxidation. Therefore, we study the effect of in vitro supplementation of Nigella sativa oil and honey on sperm parameters; motility, progressive score, and abnormalities in fresh and post-thawed goat semen. Pooled semen from five sexually matured males Jermasia goats between aged 2 to 3 years old, with average live weight of 35.9±5.7 kg and average body condition score (BCS) of 1.7±0.45 were used in this study. The semen were added with trisegg yolk extender and combination of supplements (Nigella sativa oil or/and honey), and divided into groups; Control (no supplementation), Treatment 1 (0.5% v/v Nigella sativa), Treatment 2 (2% v/v honey), and Treatment 3 (0.5% v/v Nigella sativa and 2% v/v honey). All parameters of fresh semen were immediately assessed after semen collection and parameter of post-thawed semen were assessed after 48 hours (2 days) of semen cryopreservation. The fresh sperm parameters were observed at 0, 0.5, 1, 1.5, and 2 hours after semen collection. Results showed that the motility of fresh semen at 1.5 and 2.0 hours after collection treated with Nigella sativa oil (Group 1; 73.8% and 72.0% respectively) and honey (Group 2; 73.3% and 72.0%; respectively) were 20% (1.5 h) and 8% (2.0 h) lower than Controls (93.0% and 79.8% of motility) (P<0.05). The progressive score and sperm abnormality were not significant between groups. While, the motility of post-thawed semen was higher (P<0.05) in treatment 3 (60.33±10.08 of motility; supplemented with Nigella sativa and honey) compare to Controls (24.33±8.17 of motility) at 0 and 0.5 hour after thawing. Thus, we suggested that Nigella sativa oil and honey show a potential as a supplement to goat's extender with the ability to protect sperm membrane from damage due to oxidative stress and could prevent ice crystal formation during cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms

Maidin

Padlan

Azuan

et al. 2018

Trop. Anim. Sci. J.

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“…In recent years, extensive research has been conducted to investigate the effect of natural (of herbal, natural and plant origins) antioxidants such as ginseng (Park et al, 2016), curcumin (Shah et al, 2016), date palm pollen grains (El-Sheshtawy et al, 2014), honey bee (El-Sheshtawy et al, 2016a) and pomegranate juice (El-Sheshtawy et al, 2016b) on the viability of animal sperm during cooling and cryopresevation. DPPG is a fine powder material produced by male flowering date palm plants, necessary for plants pollination.…”

Section: Discussionmentioning

confidence: 99%

Effects of Phoenix dactylifera pollen grains extract supplementation on post-thaw quality of Arabian stallion semen

El-Sisy¹,

El-Badry²,

El-Sheshtawy³

et al. 2018

BJVM

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-Nattat, 2018. Effects of Phoenix dactylifera pollen grains extract supplementation on post-thaw quality of Arabian stallion semen. Bulg. J. Vet. Med., 21, No 1,[40][41][42][43][44][45][46][47][48][49] This study explored the effect of extender supplementation with different concentrations of date palm pollen grain (DPPG) on post-thawed sperm motility, viability index, membrane and acrosome integrities in Arabian stallions. Five ejaculates from each of four Arabian stallions were subjected to cryopreservation with a modified INRA-82, without any supplementation (control) or supplemented with 50, 100, 150, 200 and 250 mg DPPG. After thawing, all samples were maintained at 37 o C, while analyses were performed at 0, 1, and 2 and 3 hours. Sperm motility percentage, viability index, membrane integrity percentage and acrosome integrity of each sample were determined by conventional laboratory methods. The addition of 100 mg DPPG resulted in improved maintenance of sperm motility after 0 and 60 min post-thawing, as compared to the control and other treatment groups. Nonsignificant effects on viability index were observed after enrichment of extender with 100 and 150 mg DPPG. The addition of 100 and 150 mg DPPG resulted in significant (P<0.0010) improvement in post-thawing membrane integrity (41.33±0.83%; 41.33±2.33%) compared to the controls (34.33±1.55%). These concentrations exerted also a beneficial effect in preserving sperm acrosome integrity (38.33±1.01%; 38.67±1.64%) as compared with the control one (33.33±1.12%). Supplementation of modified INRA-82 with 200 mg DPPG failed to maintain sperm motiliy while 250 mg PG has a negative impact on all studied post-thawing semen parameters. In conclusion, adding 100 and 150 mg date palm pollen grain extract to modified INRA-82 seemed useful in the chilling and freezing process of Arabian stallion sperm.

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“…On the other hand, El-Battawy and Brannas (2015) indicated that semen extenders containing propolis achieved the best motility, cryoprotection and maintained sperm cells integrity. In addition, El-Sheshtawy et al (2016) mentioned that addition of bee products (as honey and propolis solution) in stallion semen extender improved storing, frozen sperm activity and also attended superior conception rate. Similarly, Khalifa et al (2016) reported that propolis extract (as powder or glue) in ram semen extenders had greater elimination of bacterial contamination than synthetic antibiotics such as Pen-Strep, Alamycin and Vetrocin.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effects of Propolis on Preservation of Ram's Semen Extender and Its Fertility Rate

Mohamed

2017

Journal of Animal and Poultry Production

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The present study aimed to define the effect of different concentrations of propolis powder or glue extract compared with synthetic antibiotic on ram's semen quality, resistance to bacterial contamination and fertility rate. Semen samples of six mature rams aging 2.2 years old with an average 62.0 kg live body weight were collected twice a week for three weeks using artificial vagina. The semen ejaculates were pooled, extended and divided into seven portions. The final extension rate was 1 semen: 10 extender. The seven portions were (CTL, without any antibiotic type and kept as control group), 400µl pen-step (SA 400µl) and 600µl pen-step (SA 600µl) as synthetic antibiotic; 400µl and 600µl propolis powder (PP 400µl & PP 600µl); 400µl and 600µl propolis glue (PG 400µl & PG 600µl) as natural antibiotic, respectively. Extended semen samples were stored at 5 °C for 6 days. The obtained results showed that, spermatozoa quality was significantly (P<0.05) decreased with the advancement of storage period in all extended samples. In addition, sperm parameters (sperm motility, acrosomal status and normal spermatozoa), enzymatic activities (ALT, AST, ALP and LDH), bacterial count and fertility rate were improved (P<0.05) in extended samples treated with synthetic and natural antibiotics either powder or glue than the control. A higher antimicrobial activity after 24 hrs incubation for PP 600µl and PG 600µl extenders were significantly (P<0.05) decreased compared with other extended samples. Moreover, the PG 600µl antibiotic was insignificantly greater litter size (1.27%) compared with (1.07%) for SA 600µl one. Regarding to the results of this study, it could be concluded that antimicrobial effect of natural propolis either powder or glue in extended ram semen was capable to improve ram semen quality and inhibitory bacterial zones, as well as, improvement fertility rate.

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Natural honey as a cryoprotectant to improve Arab stallion post-thawing sperm parameters

Cited by 26 publications

References 15 publications

Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms

Supplementation of Nigella sativa Oil and Honey Prolong the Survival Rate of Fresh and Post-Thawed Goat Sperms

Effects of Phoenix dactylifera pollen grains extract supplementation on post-thaw quality of Arabian stallion semen

Antimicrobial Effects of Propolis on Preservation of Ram's Semen Extender and Its Fertility Rate

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