Antimicrobial Effects of Propolis on Preservation of Ram's Semen Extender and Its Fertility Rate

Mohamed, M. Y.

doi:10.21608/jappmu.2017.45857

Cited by 6 publications

(7 citation statements)

References 24 publications

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“…Also, there were significant (P < 0.01) increases in the spillage of alanine transaminase, aspartate transaminase, alkaline phosphatase and lactic dehydrogenase enzymes into the extracellular medium through preservation time. These finding are similar to those obtained by Mohamed (2017) and Mohamed et al (2019).…”

Section: Discussionsupporting

confidence: 93%

Usage of Some Natural or Synthetic Compounds as Antioxidant and their Effects on Cryopreservation and Penetration of Ram Spermatozoa

Diab¹,

Ahmed²,

Fahim³

et al. 2021

AAVS

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T he chilling of semen has some adverse effects on sperm properties. Modifying dilators with traditional and untraditional refrigerants can influence sperm properties (Mohamed et al., 2020). Preservation of semen alters sperm structure and biochemical properties and impedes fertilization (Mohamed et al., 2019).The addition of antioxidants to semen diluents could be extended storage time, improve motility, decrease cellu-lar damage, protected acrosomal membrane and raise the viability and fertilization ability of spermatozoa. Among substances with recognized antioxidants effectiveness were natural or synthetic to be added to extenders. In this context, vitamin E (synthetic non-enzymatic antioxidant) is one of the most principal lipid-soluble defensive antioxidants and has a major role as an antioxidant. Hence, Ahmadi Hamedani et al. (2016) indicated that supplementation 2mM of vitamin E as an antioxidant is suggested to improve cryopreservation techniques in ram sperm. Moreover, Motemani et al. (2017) demonstrated that concentrations research Article Abstract | The current investigation was performed for determining the impact of natural (rosemary and ginseng aqueous extracts) and synthetic (L-carnitine and vitamin E) antioxidants on ram sperm characteristics during three sessions (preservation, equilibration, and frozen-thawing) using six mature Ossimi rams. Semen collection was performed twice a week for three weeks by an artificial vagina. In different sessions, ejaculates were pooled split into five aliquots. The natural (rosemary and ginseng aqueous extracts) and synthetic (L-carnitine and vitamin E) antioxidants were supplemented 20 mg/ml of each to the first four portions G1, G2, G3, and G4, respectively. However, the fifth part (G5) served as control without any antioxidant type. The results showed that sperm characteristics, sperm recovery rate and reduced glutathione (GSH) were higher (P < 0.05), but the concentration of lipid peroxidation (LPX) and enzymatic activities (ALT, AST, ALP, and LDH) were lower (P < 0.05) in G1, G2, G3 and G4 than G5 extenders post-equilibration and thawing conditions. Furthermore, the penetration ability into ewe cervical mucus was significantly (P < 0.05) better in the extended ram semen with natural antioxidant (G1 & G2) than with synthetic antioxidant (G3 & G4). The findings of this study indicated that extenders supplemented with rosemary or ginseng extract (as a natural antioxidant) could be alleviated sperm characteristics, penetration value and reduced oxidative stress marker corresponding to L-carnitine and vitamin E (as a synthetic antioxidant) during after the cooling and freezethawing processes of ram spermatozoa.

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Section: Discussionsupporting

confidence: 93%

Usage of Some Natural or Synthetic Compounds as Antioxidant and their Effects on Cryopreservation and Penetration of Ram Spermatozoa

Diab¹,

Ahmed²,

Fahim³

et al. 2021

AAVS

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“…In harmony with our results, propolis extract in the extenders showed the highest ability to sustain the viability and motility of ram spermatozoa (Mohamed and Zanouny, 2017). Also, the percentage of live sperm with intact and detached acrosome was increased, while dead sperm with intact and detached acrosome was decreased by propolis addition in the extender of stored ram semen (Mohamed, 2017). Supplementation of semen extenders with 0.2% of propolis powder or glue increased sperm motility, livability, and normality of spermatozoa (Khalifa et al, 2016).…”

Section: Figuresupporting

confidence: 88%

“…In accordance these results, Akandi et al (2015) found that propolis supplementation with a semen extender improves sperm viability and DNA integrity. In addition, extenders containing propolis were reported to lower the release of enzymes such as ALT, AST, ALP, and LDH significantly into the sperm medium (Mohamed and Zanouny, 2017;Mohamed, 2017) which may reflect the high sperm membrane integrity during incubation and storage (Zeidan et al, 2004). Improving sperm characteristics in pre-freezing and post-thaw semen was explained by several authors.…”

Section: Figurementioning

confidence: 99%

Role of Propolis Ethanolic Extract as an Antioxidant Supplement in Tris-Extender to Enhance Semen Quality of Egyptian Buffaloes during Cryopreservation

Abdel-Khalek,

Sakr,

Nagy

et al. 2023

AAVS

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This study aimed to evaluate the effect of propolis supplementation on semen extender of Egyptian buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5-5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with Tris-extender at each level of PEE (PEE0, PEE1, PEE2, PEE3, and PEE4). Semen was gradually diluted at 37 °C with each extender at a rate of 1: 10, equilibrated for 4 hours at 4 °C, packaged in French straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37 o C for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total antioxidant capacity and superoxide dismutase activity, and decreased percentage of apoptosis, early apoptosis, necrosis, and malondialdehyde level; most favorable results were recorded with PEE2. The foregoing results indicated that supplementation of Tris-semen extender with propolis ethanolic extender at a level of 8 mg/ml increased the functional activity of spermatozoa, decreased sperm apoptosis, and necrosis, and improved the antioxidant status of sperm medium in cryopreserved semen of Egyptian buffaloes.

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“…Addition of propolis to semen ram extender (0.5 and 1.0 mg/mL; preserved at 5 °C for 48 h) enhanced sperm function, and antioxidant capacity as well as reduced the lipid peroxidation 13 . Moreover, several other studies shown a significant enhance in semen longevity in rabbits 14 , and ram 15 by propolis administration. Additionally, propolis was add to the cryopreserved semen media in fish 16 , goats 17 , bull 11 , ram 12 .…”

Section: Introductionmentioning

confidence: 81%

Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation

Abdelnour

Hassan

Shehabeldin

et al. 2023

Sci Rep

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Buffalo sperm is sensitive to cryoinjuries, thus improving sperm cryoresistance is a critical approach for wide spreading the assisted reproductive technologies in buffalo. The intention of this work was to assess the effect of propolis-loaded in nanoliposomes (PRNL) supplementation of semen extender on semen quality, antioxidant status and some apoptotic genes of cryopreserved buffalo semen. PRNL were prepared using cholesterol (Chol) as well as soybean lecithin and their physicochemical properties were characterized. Egyptian buffalo bulls (4–6 years) were involved, and the semen samples were collected using the artificial vagina method. Buffalo semen was pooled (n = 25 ejaculates) and cryopreserved in tris extender containing PRNL at 0 (PRNL0), 2 (PRNL2), 4 (PRNL4) and 6 µg/mL (PRNL6), respectively. The PRNL had a size of 113.13 nm and a negative zeta potential (− 56.83 mV). Sperm progressive motility, viability, membrane integrity, abnormalities, chromatin damage, redox status, apoptosis status, and apoptotic genes were investigated after post-thawed buffalo semen. Using 2 or 4 µg/mL PRNL significantly increased sperm progressive motility, viability, and membrane integrity, while sperm abnormalities and the percentage of chromatin damages were the lowest in PRNL2 group. Moreover, the PRNL2 group exhibited the best results for all antioxidative activities (TAC, SOD, GPx and CAT) with significantly higher levels than the other groups (P < 0.05). The levels of ROS and MDA were significantly lower in the PRLN2 compared with other groups. The sperm caspase 3 enzyme activities showed the lowest values in PRNL2 groups followed by PRNL4 and PRNL6 groups with significant differences compared with the control. Adding 2 µg/mL PRNL to freezing media significantly reduced apoptotic genes such as Bax and Caspase 3 in sperm, while significantly increase in Bcl2 expression compared with the control (P < 0.001). The expression of Bcl2, Caspase 3 and Bax genes in sperm were not affected by the 6 µg/mL PRNL addition (P > 0.05). The electron micrography descriptions exemplified that the fortification of 2 or 4 µg/mL PRNL maintained the acrosomal and plasma membrane integrities as well as sustained the ultrastructure integrity of the cryopreserved buffalo spermatozoa when compared with control group, whereas the 6 µg/mL of PRNL demonstrated highest injury to the acrosome and plasma membranes. Results show supplementation of the buffalo freezing extender with 2 or 4 µg/mL of PRNL enhanced post-thawed sperm quality via boosting the antioxidant indices, diminishing the oxidative stress and apoptosis as well as maintained the ultrastructure integrity of frozen-thawed buffalo sperm.

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Antimicrobial Effects of Propolis on Preservation of Ram's Semen Extender and Its Fertility Rate

Cited by 6 publications

References 24 publications

Usage of Some Natural or Synthetic Compounds as Antioxidant and their Effects on Cryopreservation and Penetration of Ram Spermatozoa

Usage of Some Natural or Synthetic Compounds as Antioxidant and their Effects on Cryopreservation and Penetration of Ram Spermatozoa

Role of Propolis Ethanolic Extract as an Antioxidant Supplement in Tris-Extender to Enhance Semen Quality of Egyptian Buffaloes during Cryopreservation

Effects of propolis-loaded nanoliposomes fortification in extender on buffalo semen cryopreservation

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