Natural deep eutectic systems as alternative nontoxic cryoprotective agents

Castro, Vânia I. B.; Craveiro, Rita; Silva, Joana M.; Reis, Rui L.; Paiva, Alexandre; Duarte, Ana Rita C.

doi:10.1016/j.cryobiol.2018.06.010

Cited by 96 publications

(69 citation statements)

References 53 publications

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“…Our model suggests that sugar alcohols interact with sugars for a range of molecules and concentrations tested. Castro et al () observed a similar outcome when they determined that trehalose and glycerol formed a natural deep eutectic system when combined with water. Specifically, the combination of molecules exhibited a stronger than expected influence on the eutectic temperature of the solution.…”

Section: Discussion/conclusionmentioning

confidence: 72%

“…We have shown that certain combinations of sugars, sugar alcohols and amino acids not only maintained post‐thaw recovery but also resulted in cells that had improved function compared with those that were cryopreserved with DMSO. Interactions between sugars and sugar alcohols have also been observed by other groups, and they have discovered that these molecules interact with each other and water to form deep natural eutectic systems (Castro et al, ).…”

Section: Introductionmentioning

confidence: 83%

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Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells

Dosa

et al. 2019

Biotech & Bioengineering

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This study examined the post‐thaw recovery of Jurkat cells cryopreserved in three combinations of five osmolytes including trehalose, sucrose, glycerol, mannitol, and creatine. Cellular response was characterized using low‐temperature Raman spectroscopy, and variation of post‐thaw recovery was analyzed using statistical modeling. Combinations of osmolytes displayed distinct trends of post‐thaw recovery, and a nonlinear relationship between compositions and post‐thaw recovery was observed, suggesting interactions not only between different solutes but also between solutes and cells. The post‐thaw recovery for optimized cryoprotectants in different combinations of osmolytes at a cooling rate of 1°C/min was comparable to that measured with 10% dimethyl sulfoxide. Statistical modeling was used to understand the importance of individual osmolytes as well as interactions between osmolytes on post‐thaw recovery. Both higher concentrations of glycerol and certain interactions between sugars and glycerol were found to typically increase the post‐thaw recovery. Raman images showed the influence of osmolytes and combinations of osmolytes on ice crystal shape, which reflected the interactions between osmolytes and water. Differences in the composition also influenced the presence or absence of intracellular ice formation, which could also be detected by Raman. These studies help us understand the modes of action for cryoprotective agents in these osmolyte solutions.

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Section: Discussion/conclusionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 83%

Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells

Dosa

et al. 2019

Biotech & Bioengineering

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“…The ability for the DES to supercool far below 0 °C and subsequently undergo vitrification could be beneficial for low‐temperature applications such as cryoprotection, where the amorphous nature and absence of crystallinity in the protective media is advantageous for the preservation of labile substrates. Indeed, such an approach has recently been outlined in the case of other DES systems and appears to offer a promising alternative to current preeminent cryoprotectants such as dimethyl sulfoxide, and ethylene/propylene glycols which have inherent toxicity issues …”

Section: Resultsmentioning

confidence: 99%

Deep Eutectic Solvents Based on Natural Ascorbic Acid Analogues and Choline Chloride

et al. 2020

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Deep eutectic solvents (DES) are one of the most promising green technologies to emerge in recent years given their combination of environmentally friendly credentials and useful functionalities. Considering the continued search for new DESespecially those that exemplify the aforementioned characteristics, we report the preparation of DES based on natural analogues of l-ascorbic acid for the first time. The onset of eutectic melting occurred at temperatures far below the melting point of the individual components and resulted in the generation of glass forming fluids with glass transition temperatures, viscosities and flow behavior that are comparable to similar systems. This work expands the current array of DES that can be produced using naturally occurring components, which given their potential to be bio-derived, interesting physicochemical properties (e. g. propensity to supercool and vitrify) and apparent antibacterial nature, may provide utility within a range of applications.

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“…For instance, NADESs could provide a matrix, in which the reactions involving poorly water soluble substrates/products and also the biosynthesis, storing and transporting of non‐water soluble molecules and polymers take place ,,,. Moreover, this media may allow the cells to tolerate adverse conditions, including low and high temperatures, desiccation and salt stress ,,,. As a natural strategy of survival of some extreme‐cold tolerant animals, the concentration of DES components as metabolites increase unexpectedly under extreme cold conditions .…”

Section: Resultsmentioning

confidence: 99%

“…As a natural strategy of survival of some extreme‐cold tolerant animals, the concentration of DES components as metabolites increase unexpectedly under extreme cold conditions . The role of DESs in bacterial cryoprotection and preservation has also been elucidated ,, DESs have also been suggested to provide an environment in which metabolism occurs under extreme conditions ,.…”

Section: Resultsmentioning

confidence: 99%

Deep Eutectic Solvents as a New Generation of Chemical Chaperones

et al. 2018

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Despite research efforts to unravel the role of naturally occurring deep eutectic solvents (NADESs) in living cells, there are still many questions left unanswered. The proposed hypothesis about the role of NADESs in cryopreservation and resistance of cells motivated us to investigate whether DESs might possess chaperoning activity. Accordingly, here, the chaperone‐like activity of a well‐known DES, urea‐choline chloride DES (UC), on a model protein, lysozyme, has been evaluated. The results showed that UC DES was not only capable of suppressing lysozyme aggregation almost completely, but also efficiently enhanced the refolding yield of the denatured enzyme by nearly 95%, thereby suggesting the chaperoning potency of DESs. The refolding promotion of lysozyme was also verified through fluorescence study. The results may introduce DESs as new generation chaperones and shed light on their role in cell resistance under stress conditions.

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Natural deep eutectic systems as alternative nontoxic cryoprotective agents

Cited by 96 publications

References 53 publications

Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells

Characterizing modes of action and interaction for multicomponent osmolyte solutions on Jurkat cells

Deep Eutectic Solvents Based on Natural Ascorbic Acid Analogues and Choline Chloride

Deep Eutectic Solvents as a New Generation of Chemical Chaperones

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