Deep Eutectic Solvents as a New Generation of Chemical Chaperones

Niknaddaf, Forouzan; Shahangian, S. Shirin; Heydari, Akbar; Hosseinkhani, Saman; Sajedi, Reza H.

doi:10.1002/slct.201802235

Cited by 10 publications

(29 citation statements)

References 45 publications

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“…195 The fact that many DESs contain natural primary metabolites including sugars, sugar alcohols, organic acids, amino acids, and amines (which are thought to aid in survival at negative temperatures) has inspired researchers to begin to explore DESs in innovative ways, such as cryoprotective agents inspired by the NADES-like compounds in plants which help prevent the formation of ice in cells, 196 room-temperature storage media for therapeutic proteins, 197 and as artificial, small-molecule chaperonins aiding in protein refolding. 198 Overall, these results demonstrate that the unique properties of DESs (e.g., low water activity, high ionic strength, molecular crowding, and ultrahigh viscosity) open exciting possibilities for controlling nucleic acid structure, stability, and for deploying catalytic, biosensory, and enzyme−nucleic acid complexes within DESs. We refer interested readers to more detailed accounts on this topic published previously.…”

Section: Genomics/fundamentals Of Nucleic Acidsmentioning

confidence: 72%

“…As an additional example, Marrucho and co-workers recently introduced a DES comprising a 1:1 molar mixture of choline chloride with acetic acid as a promoter of fast lysozyme fibrillation . The fact that many DESs contain natural primary metabolites including sugars, sugar alcohols, organic acids, amino acids, and amines (which are thought to aid in survival at negative temperatures) has inspired researchers to begin to explore DESs in innovative ways, such as cryoprotective agents inspired by the NADES-like compounds in plants which help prevent the formation of ice in cells, room-temperature storage media for therapeutic proteins, and as artificial, small-molecule chaperonins aiding in protein refolding . Overall, these results demonstrate that the unique properties of DESs ( e.g.…”

Section: Selected Applications Of Deep Eutectic Solventsmentioning

confidence: 99%

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Deep Eutectic Solvents: A Review of Fundamentals and Applications

et al. 2020

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Deep eutectic solvents (DESs) are an emerging class of mixtures characterized by significant depressions in melting points compared to those of the neat constituent components. These materials are promising for applications as inexpensive "designer" solvents exhibiting a host of tunable physicochemical properties. A detailed review of the current literature reveals the lack of predictive understanding of the microscopic mechanisms that govern the structure−property relationships in this class of solvents. Complex hydrogen bonding is postulated as the root cause of their melting point depressions and physicochemical properties; to understand these hydrogen bonded networks, it is imperative to study these systems as dynamic entities using both simulations and experiments. This review emphasizes recent research efforts in order to elucidate the next steps needed to develop a fundamental framework needed for a deeper understanding of DESs. It covers recent developments in DES research, frames outstanding scientific questions, and identifies promising research thrusts aligned with the advancement of the field toward predictive models and fundamental understanding of these solvents.

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Section: Genomics/fundamentals Of Nucleic Acidsmentioning

confidence: 72%

Section: Selected Applications Of Deep Eutectic Solventsmentioning

confidence: 99%

Deep Eutectic Solvents: A Review of Fundamentals and Applications

et al. 2020

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“…Monitoring of the intrinsic fluorescence of tryptophan confirmed that the solvents promoted correct refolding. The biological activity of the enzyme, again measured by the ability to lyse bacteria, was also recovered better when the solvent was present in the renaturation solution (Niknaddaf et al, 2018).…”

Section: The Influence Of Choline-based Ionic Liquids On the Catalytic Activity Of Lipase Frommentioning

confidence: 97%

“…In living cells, protein folding and re-folding is facilitated by other proteins known as chaperones that play an important role in the response of the cell to stress. The fact that many of the components of deep eutectic solvents are found in cells and are related to survival under extreme condition led Niknaddaf et al to put forward the hypothesis that these solvents could act as chemical chaperones (Niknaddaf et al, 2018). They conducted experiments to test this using a model of lysozyme aggregation by the reducing agent dithiothreitol.…”

Section: The Influence Of Choline-based Ionic Liquids On the Catalytic Activity Of Lipase Frommentioning

confidence: 99%

Deep Eutectic Solvents for Innovative Pharmaceutical Formulations

Nguyen

Augis

Fourmentin

et al. 2020

Environmental Chemistry for a Sustainable World

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Finding alternative solvents for industrial processes, such as chemical synthesis or extraction of biologically active molecules that are less toxic and more environmentally friendly than the organic solvents used up to now, is a major societal issue. These alternative solvents are often described as "green, biodegradable" solvents. Deep eutectic solvents, which are mixtures of simple and often naturally occurring compounds, have been extensively studied in this regard. Among their possible applications, there has been increasing interest in their use for the preparation of pharmaceutical formulations. Indeed, by changing the nature and ratio of their components, deep eutectic solvents can be adapted to a wide range of active molecules, from poorly soluble small molecules to labile macromolecules. The use of deep eutectic solvents to solubilize active molecules with low aqueous solubility and/or low permeability could be an alternative approach to increase their dissolution and in-vivo absorption. This could result in significant increases of bioavailability or enhanced therapeutic efficacy of currently marketed drugs. Moreover, deep eutectic solvents can be used to limit phenomena like polymorphism or degradation which present a challenge to drug formulation. However, despite being generally described as biodegradable and nontoxic due to the nature of their constituents, the safety of deep eutectic solvents, which possess both novel physico-chemical and biological properties, cannot be taken for granted and must therefore be carefully studied during development stages. Therefore, this chapter presents not only recent progress in the application of deep eutectic solvents in the development of formulations for improving therapeutic efficacy by different routes of administration but also studies that have been undertaken to investigate the toxicity of deep eutectic solvents to both living organisms and the environment.

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“…13,14 Due to these properties, ILs have begun to supersede conventional organic solvents. 15,16 Nonetheless, ILs suffer from several limitations such as costly synthesis, toxicity and challenging preparation besides having potential environmental impacts. 16…”

Section: Introductionmentioning

confidence: 99%