Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing: A review

Gaspar‐Pintiliescu, Alexandra; Stanciuc, Ana-Maria; Crăciunescu, Oana

doi:10.1016/j.ijbiomac.2019.07.155

Cited by 252 publications

(147 citation statements)

References 125 publications

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“…Recently, the use of collagen loaded with bioactive compounds derived from plants for design of natural wound dressings classified as sponges, electrospun nanofibre matrices, films and hydrogels was investigated to overcome the toxicity and reduced antimicrobial activity of existing products [17]. In the present paper, collagen hydrolysate was used in high concentration due to the associative properties of collagen hydroysate molecules with effect on increased viscosity and improved electrospinnable properties.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

et al. 2020

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This research aimed to obtain biocompatible and antimicrobial nanofibres based on concentrated collagen hydrolysate loaded with thyme or oregano essential oils as a natural alternative to synthesis products. The essential oils were successfully incorporated using electrospinning process into collagen resulting nanofibres with diameter from 471 nm to 580 nm and porous structure. The presence of essential oils in collagen nanofibre mats was confirmed by Attenuated Total Reflectance -Fourier Transform Infrared Spectroscopy (ATR-FTIR), Ultraviolet–visible spectroscopy (UV–VIS) and antimicrobial activity. Scanning Electron Microscopy with Energy Dispersive Spectroscopy analyses allowed evaluating the morphology and constituent elements of the nanofibre networks. Microbiological tests performed against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans showed that the presence of essential oils supplemented the new collagen nanofibres with antimicrobial properties. The biocompatibility of collagen and collagen with essential oils was assessed by in vitro cultivation with NCTC clone 929 of fibroblastic cells and cell viability measurement. The results showed that the collagen and thyme or oregano oil composites have no cytotoxicity up to concentrations of 1000 μg·mL−1 and 500 μg mL−1, respectively. Optimization of electrospinning parameters has led to the obtaining of new collagen electrospun nanofibre mats loaded with essential oils with potential use for wound dressings, tissue engineering or protective clothing.

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Section: Introductionmentioning

confidence: 99%

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

et al. 2020

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“…Wound dressing is used to stimulate and accelerate skin tissue repair. An ideal dressing should adhere well to the wound interface, maintain a balanced moist environment, allow gaseous exchanges, remove excess exudates, and act as a protective barrier against microorganisms [ 1 , 2 ]. To cater for all aspects of wound care treatment and its complexity, various models of a wound dressing with different morphologies and properties have been created such as alginate, collagen, and chitosan to be used as films, foams, hydrogels, hydrocolloids, or scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Characterization of Bilayer Hybrid Nanocellulose-Collagen as a Potential Wound Dressing

et al. 2020

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The eminent aim for advance wound management is to provide a great impact on the quality of life. Therefore, an excellent strategy for an ideal wound dressing is being developed that eliminates certain drawbacks while promoting tissue regeneration for the prevention of bacterial invasion. The aim of this study is to develop a bilayer hybrid biomatrix of natural origin for wound dressing. The bilayer hybrid bioscaffold was fabricated by the combination of ovine tendon collagen type I and palm tree-based nanocellulose. The fabricated biomatrix was then post-cross-linked with 0.1% (w/v) genipin (GNP). The physical characteristics were evaluated based on the microstructure, pore size, porosity, and water uptake capacity followed by degradation behaviour and mechanical strength. Chemical analysis was performed using energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectrophotometry (FTIR), and X-ray diffraction (XRD). The results demonstrated a uniform interconnected porous structure with optimal pore size ranging between 90 and 140 μm, acceptable porosity (>70%), and highwater uptake capacity (>1500%). The biodegradation rate of the fabricated biomatrix was extended to 22 days. Further analysis with EDX identified the main elements of the bioscaffold, which contains carbon (C) 50.28%, nitrogen (N) 18.78%, and oxygen (O) 30.94% based on the atomic percentage. FTIR reported the functional groups of collagen type I (amide A: 3302 cm−1, amide B: 2926 cm−1, amide I: 1631 cm−1, amide II: 1547 cm−1, and amide III: 1237 cm−1) and nanocellulose (pyranose ring), thus confirming the presence of collagen and nanocellulose in the bilayer hybrid scaffold. The XRD demonstrated a smooth wavy wavelength that is consistent with the amorphous material and less crystallinity. The combination of nanocellulose with collagen demonstrated a positive effect with an increase of Young’s modulus. In conclusion, the fabricated bilayer hybrid bioscaffold demonstrated optimum physicochemical and mechanical properties that are suitable for skin wound dressing.

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“…Gelatin is a heterogeneous mixture of proteins derived from collagen hydrolysis. It is extracted from the connective tissues, skins and bones of animals, such as porcine, bovine, and fish (Gaspar‐Pintiliescu, Stanciuc, & Craciunescu, 2019). Gelatin has reversible sol–gel conversion property, good biocompatibility, and biodegradability with low antigenicity (Chen & Vyazovkin, 2009; Taokaew, Seetabhawang, Siripong, & Phisalaphong, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Especially, because gelatin has good film‐forming property, so elastic, thin gelatin films can be prepared by drying gelatin solutions with or without crosslinking (Chattopadhyay & Raines, 2014). These gelatin films can absorb wound exudates and provide moist environment for a wound to accelerate wound healing (Gomez‐Guillen, Gimenez, Lopez‐Caballero, & Montero, 2011), so they have been used as important fundamental materials in wound dressings (Gaspar‐Pintiliescu et al, 2019). Gelatin films also can load natural extracts or growth factors to improve the wound healing (Jridi et al, 2017; Kavoosi, Dadfar, & Purfard, 2013; Tanaka, Nagate, & Matsuda, 2005).…”

Section: Introductionmentioning

confidence: 99%

Study on the stability and cellular affinity of gelatin‐polysaccharide composite films

Liu

Gao

et al. 2020

J Biomedical Materials Res

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The gelatin film has great potential in biomedical applications, especially in wound healing. The combination of gelatin films and stem cells could further accelerate the skin regeneration. Although polysaccharide modification can improve the mechanical property and biological activity of gelatin films, information about the stability and cellular affinity is still limited. This study investigated the influence of polysaccharides on the stability and cellular affinity of gelatin films. Two kinds of gelatin‐polysaccharide composite films, including gelatin‐hyaluronic acid (G‐HA) and gelatin‐chitosan (G‐CS), were prepared in this study. It was found that G‐HA composite film had better short‐term and long‐term stability compared with G‐CS composite film. And G‐HA composite film also had better biological safety than G‐CS film. Moreover, the surface of G‐HA composite film supported the adhesion and growth of human umbilical cord Wharton's jelly‐derived mesenchymal stem cells (WJ MSCs) better than G‐CS film surface. These data illustrated that G‐HA composite film has better stability and cellular affinity compared with G‐CS film, which could be considered a promising delivery system of stem cells for further in vivo studies. Therefore, this work would be very helpful to optimize the preparation of gelatin‐polysaccharide composite films.

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Natural composite dressings based on collagen, gelatin and plant bioactive compounds for wound healing: A review

Cited by 252 publications

References 125 publications

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

Bioactive Properties of Nanofibres Based on Concentrated Collagen Hydrolysate Loaded with Thyme and Oregano Essential Oils

Physicochemical Characterization of Bilayer Hybrid Nanocellulose-Collagen as a Potential Wound Dressing

Study on the stability and cellular affinity of gelatin‐polysaccharide composite films

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