2016
DOI: 10.1590/s1984-29612016071
| View full text |Cite
|
Sign up to set email alerts
|

Abstract: Leishmaniasis is a major public health problem worldwide. Because Leishmania can adapt to new hosts or vectors, knowledge concerning the current etiological agent in dogs is important in endemic areas. This study aimed to identify the Leishmania species detected in 103 samples of peripheral blood from dogs that were naturally infected with these protozoa. The diagnosis of leishmaniasis was determined through parasitological examination, the indirect enzyme-linked immunosorbent assay (ELISA) and the polymerase … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
18
0
2

Year Published

2017
2017
2023
2023

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 24 publications
(20 citation statements)
references
References 27 publications
0
18
0
2
Order By: Relevance
“…Notably, Sanches et al [28] showed a high percentage of L. ( L. ) amazonesis infection in naturally infected dogs in an endemic area, underlining the necessity to discriminate these two species not only in human but also in veterinary medicine. Since HRM analysis resulted inconclusive for discrimination of L. ( L. ) infantum and L. ( L. ) amazonensis in about 53% of samples, a new SYBR-green qPCR assay (qPCR-ama) was designed to amplify a minicircle subclass preponderant in L. ( L. ) amazonensis, rather than targeting a hypothetical species-specific sequence.…”
Section: Discussionmentioning
confidence: 99%
“…Notably, Sanches et al [28] showed a high percentage of L. ( L. ) amazonesis infection in naturally infected dogs in an endemic area, underlining the necessity to discriminate these two species not only in human but also in veterinary medicine. Since HRM analysis resulted inconclusive for discrimination of L. ( L. ) infantum and L. ( L. ) amazonensis in about 53% of samples, a new SYBR-green qPCR assay (qPCR-ama) was designed to amplify a minicircle subclass preponderant in L. ( L. ) amazonensis, rather than targeting a hypothetical species-specific sequence.…”
Section: Discussionmentioning
confidence: 99%
“…Leishmania species compatibility was evaluated according to a previously described melting curve analysis [19], and subgenus confirmation was performed using a conventional PCR to amplify fragments of the ITS1 region, followed by restriction fragment length polymorphism (PCR-RFLP) analysis by HaeIII digestion as previously described [22,23]. All reactions were performed by a researcher blinded to the other exams and to the patients' clinical conditions.…”
Section: Index Test (Qualitative and Quantitative Qpcr)mentioning
confidence: 99%
“…Determination of the Leishmania species was performed by PCR-RFLP (Restriction Fragment Length Polymorphism) [20], comparing the restriction profiles of the sample with a PCR restriction profile obtained from L . infantum (IOC / L0575-MHOM / BR / 2002 / LPC-RPV), L .…”
Section: Methodsmentioning
confidence: 99%