Natural antibodies and their significance in active immunization and protection against a defined pathogen in fish

Sinyakov, Michael S.; Dror, Moti; Zhevelev, Helena M.; Margel, Shlomo; Avtalion, Ramy R.

doi:10.1016/s0264-410x(02)00379-1

Cited by 105 publications

(77 citation statements)

References 23 publications

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“…Increases in total immunoglobin were also observed in greasy groupers (Epinephelus tauvina) fed with herbal diet with purified active ingredient of Ocimum sanctum, W. somnifera, and Myristica fragrans (Sivaram et al 2004) and Siberian sturgeon (Acipenser baerii) fed with epin (a plant growth regulator epibrassinopid) (Kolman 2001). Natural antibody is produced in certain B cells in mammalian system (Casali and Schettino 1996), and it is involved in both viral and bacterial defense of rainbow trout and goldfish (Gonzalez et al 1989;Sinyakov et al 2002). The increased total serum antibody observed in the present study could be related to the stimulation of MOS and inulin.…”

Section: Resultsmentioning

confidence: 48%

Use of food waste as fish feeds: effects of prebiotic fibers (inulin and mannanoligosaccharide) on growth and non-specific immunity of grass carp (Ctenopharyngodon idella)

Cheng

Choi

et al. 2015

Environ Sci Pollut Res

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The effects of inulin and mannanoligosaccharide (MOS) on the growth performance and non-specific immunity of grass carp were studied. Two doses of prebiotic fiber with 0.2 or 2% of the fibers are being mixed into fish feed pellets. Fish growth as well as selected non-specific immune parameters of grass carp were tested in a feeding trial, which lasted for 8 weeks. Fish was fed at 2.5% body mass per day. INU02, INU2, and MOS2 significantly improved relative weight gain, specific growth rate, protein efficiency ratio, and food conversion ratio of grass carp fed with food waste-based diet. In terms of non-specific immune response, grass carp showed significant improvement in all three tested parameters (total serum immunoglobin, bactericidal activity, and anti-protease activity). Adding 2% of inulin (INU2) into food waste diets seemed to be more preferable than other supplemented experimental diets (INU02, MOS02, MOS2), as it could promote growth of grass carp as well as improving the non-specific immune systems of grass carp.

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Section: Resultsmentioning

confidence: 48%

Use of food waste as fish feeds: effects of prebiotic fibers (inulin and mannanoligosaccharide) on growth and non-specific immunity of grass carp (Ctenopharyngodon idella)

Cheng

Choi

et al. 2015

Environ Sci Pollut Res

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“…NAbs against the bacterium Aeromonas salmonicida have been isolated from unexposed goldfish. Sinyakov, Droret al [46] divided unexposed goldfish into high and low natural antibody groups, and when challenged with A. salmonicida, the high natural antibody group was largely protected while infections in the low natural antibody group resulted in 100% morbidity. Some authors have even hypothesised that a NAb response will predict the strength of the adaptive immune response [47].…”

Section: Discussionmentioning

confidence: 99%

Presence of antibodies to Salmonella in tuatara (Sphenodon punctatus) sera

Middleton

Nelson

Gartrell

et al. 2015

Comparative Immunology, Microbiology and Infectious Diseases

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“…However, goldfish with high levels of natural antibody activity against the A-layer protein were largely protected against experimental infection with an atypical strain of Aeromonas salmonicida (Sinyakov et al 2002). Furthermore, a significant positive correlation between survival rates and corresponding antibody levels to the A-layer protein was found in Atlantic salmon vaccinated with commercial oil-adjuvanted furunculosis vaccines (Midtlyng et al 1996).…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Vaccine efficacy in spotted wolffish Anarhichas minor: relationship to molecular variation in A-layer protein of atypical Aeromonas salmoncida

Lund¹,

Espelid²,

Mikkelsen³

2003

Dis. Aquat. Org.

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Atypical Aeromonas salmonicida strains comprise a heterogeneous group in terms of molecular and phenotypic characteristics. They cause various conditions of ulcer diseases or atypical furunculosis and are being isolated in increasing number from various fish species and geographical areas. Several marine fish species susceptible to atypical A. salmonicida, including spotted wolffish Anarhichas minor O., are now being farmed and new vaccines may be needed. A commercial furunculosis vaccine for salmon is reported to protect wolffish poorly against experimental challenge with atypical A. salmonicida. The protective antigen(s) in furunculosis vaccines is still unclear, but in oiladjuvanted vaccine for Atlantic salmon Salmo salar L., the surface A-layer was shown to be important for protection. In spotted wolffish, the efficacy of atypical furunculosis vaccines seems to vary with the atypical A. salmonicida strains used as bacterin in the vaccine. In the present study we investigated whether differences in the A-layer protein among atypical strains might be responsible for the observed variation in vaccine efficacy. Atypical A. salmonicida strains from 16 fish species in 11 countries were compared by genome polymorphism analysis using amplified fragment length polymorphism (AFLP) fingerprinting and by comparative sequencing of the vapA genes encoding the A-protein. The A-protein sequences appeared to be highly conserved except for a variable region between Residues 90 to 170. Surprisingly, the grouping of strains based on AFLP-or A-protein sequence similarities was consistent. In addition, serological differences in the A-protein among the strains were demonstrated by an A-protein-specific monoclonal antibody. Vaccines based on atypical A. salmonicida strains possessing genetically and serologically different A-layer proteins were shown to result in significantly different protection in spotted wolffish. KEY WORDS: Atypical Aeromonas salmonicida · Amplified fragment length polymorphism · AFLP · A-layer protein · Vaccine · Spotted wolffish Resale or republication not permitted without written consent of the publisherDis Aquat Org 56: [31][32][33][34][35][36][37][38][39][40][41][42] 2003 (PFGE) (Umelo & Trust 1998, O'hIci et al. 2000 and amplified fragment length polymorphism analysis (AFLP) (Lund et al. 2002a) have been used to demonstrate genetic diversity among the atypical strains. However, these methods are not comparable (Austin et al. 1998), and a reliable method for classification of atypical strains is still lacking.Several marine fish species susceptible to atypical Aeromonas salmonicida infection are now being farmed, and vaccine development will be necessary. It is not known if species-specific vaccines are needed, or if vaccines that protect against all genotypes of atypical A. salmonicida in various fish species can be developed. For the past 10 yr furunculosis in Atlantic salmon Salmo salar L. has been successfully controlled by the use of highly efficient oil-adjuvanted vaccines. Commercia...

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Natural antibodies and their significance in active immunization and protection against a defined pathogen in fish

Cited by 105 publications

References 23 publications

Use of food waste as fish feeds: effects of prebiotic fibers (inulin and mannanoligosaccharide) on growth and non-specific immunity of grass carp (Ctenopharyngodon idella)

Use of food waste as fish feeds: effects of prebiotic fibers (inulin and mannanoligosaccharide) on growth and non-specific immunity of grass carp (Ctenopharyngodon idella)

Presence of antibodies to Salmonella in tuatara (Sphenodon punctatus) sera

Vaccine efficacy in spotted wolffish Anarhichas minor: relationship to molecular variation in A-layer protein of atypical Aeromonas salmoncida

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