Natural and induced antibodies contribute to differential susceptibility to secondary cystic echinococcosis of Balb/c and C57Bl/6 mice

Mourglia‐Ettlin, Gustavo; Cucher, Marcela Alejandra; Arbildi, Paula; Rosenzvit, Mara Cecília; Dematteis, Sylvia

doi:10.1016/j.imbio.2015.07.016

Cited by 20 publications

(17 citation statements)

References 79 publications

(108 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…in 1991 19 : when activated oncospheres were injected intraperitoneally into Balb/cJ, DBA/2 J and CF-1 mice, cysts were restricted to the peritoneal cavity; activated oncospheres injected intravenously, however, lodged almost exclusively in the lung and thoracic cavity, except in DBA/2 J mice where 55% lodged in the liver. Different strains also exhibited different susceptibilities to secondary CE 20,21 . Our model of PSC injection has also the particular advantage of accurately mimickings secondary echinococcosis caused by local protoscolex spillage in the liver after spontaneous or therapeutic rupture of the cysts in humans.…”

Section: Discussionmentioning

confidence: 99%

“…Similar studies in CE patients hadalso shown that CD3 + T cells, CD3 + CD56 + natural killer T cells (NKT) and B cells were the most frequent infiltrating cells at the periphery of hepatic CE lesions; the predominance of CD3 + -immunostained cells with weak expression of CD4 + and CD8 + subtypes in CE biopsies also highlighted the role CD3 + CD56 + NKTs in the partial protection towards E. granulosus metacestode growth by the host’s immune system; it might be involved in the differences in cyst size we observed between the LDG and the HDG 29 . The persistent presence of B cells suggested a secretion of antibodies by local B cells in the liver 20 ; this could explain why specific antibodies (used for CE diagnosis) are more frequently found in patients with liver cysts than in lung cysts where the AL is less cellular 3 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model

Zhang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

The local immune mechanisms responsible for the establishment and development of Echinococcus granulosus sensu stricto infection in the liver, have been little explored. We developed a suitable experimental model that mimics naturally infected livers using portal injection of protoscoleces. Opposite to Echinococcus multilocularis infection which is dose-dependent, fully mature hydatid cysts can be established in the liver whatever the injection dose; although most of the infection sites were seen at the establishment phase as inflammatory granulomas associated with fibrosis, they never matured into cysts. At the establishment phase, a strong immune response was composed of T and B cells, with T1-type, T2-type cells and cytokines and IL-10-secreting CD8+ T cells in the liver. At the established phase, results suggested a local production of antibodies by B cells, and an involvement of NK and NKT cells. Infection outcome and local immune response in the liver, were different in the mouse models of Echinococcus granulosus sensu stricto and Echinococcus multilocularis respectively; however, only early specificities at the microenvironment level might explain the major differences found between the lesions induced by the two species. Our quantitative experimental model appears fully appropriate to further study this microenvironment and its relationship with each cestode species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model

Zhang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, it is not known whether E. granulosus infection impacts on the gut microbiota of humans or mice. Mice have been used for E. granulosus larval infection including primary ( Zhang et al, 2001 ) and secondary infection ( Gottstein, 2001 ; Mourglia-Ettlin et al, 2016 ). Mice models play an important role in studies of developmental biology and host specificity in echinococcosis ( Nakaya et al, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Echinococcus granulosus Infection Results in an Increase in Eisenbergiella and Parabacteroides Genera in the Gut of Mice

et al. 2018

View full text Add to dashboard Cite

Cystic echinococcosis (CE) is a chronic infectious disease caused by Echinococcus granulosus. To confirm whether the infection impacts on the gut microbiota, we established a mouse model of E. granulosus infection in this study whereby BALB/c mice were infected with micro-cysts of E. granulosus. After 4 months of infection, fecal samples were collected for high-throughput sequencing of the hypervariable regions of the 16S rRNA gene. Sequence analysis revealed a total of 13,353 operational taxonomic units (OTUs) with only 40.6% of the OTUs having genera reference information and 101 of the OTUs were significantly increased in infected mice. Bioinformatics analysis showed that the common core microbiota were not significantly changed at family level. However, two genera (Eisenbergiella and Parabacteroides) were enriched in the infected mice (PAMOV A < 0.05) at genus level. Functional analysis indicated that seven pathways were altered in the E. granulosus Infection Group compared with the Uninfected Group. Spearman correlation analysis showed strong correlations of IgG, IgG1 and IgG2a with nine major genera. E. granulosus cyst infection may change the gut microbiota which may be associated with metabolic pathways.

show abstract

“…The lack of a strong protective response in Balb/c has been associated with a reduced ability to express the Th1 cytokine (IFN-γ and IL-12). Whereas resistant C57BL/6 mice preferentially activate Th1 cells [16, 17]. Therefore, female and male Balb/c mice were employed in this study.…”

Section: Discussionmentioning

confidence: 99%

The assessment of xenogeneic bone immunotoxicity and risk management study

et al. 2019

View full text Add to dashboard Cite

BackgroundXenogeneic bone has been widely used in a variety of clinical bone-related disease to promote bone healing and restore bone defects. However, the adverse effects of immune system limit its application in the clinic. The aim of this study was to evaluate xenogeneic bone safety of immunotoxicity and explore the methods for immune risk supervision.ResultsXenogeneic bone, which is freeze-dried bovine cancellous bone, was implanted into the muscle of mice. On day 7, 14 and 28, the effects of xenogeneic bone were examined on humoral immunity and cellular immunity, including the levels of IgG, IgM, C3, inflammatory factors (TNF-α, IL-6), alkaline phosphatase (ALP) and the lymphocyte phenotype. The data showed that xenogeneic bone implantation had no potential to induce immune responses not only in humoral immunity but also in cellular immunity. To reveal the risk of immunogenicity, the residual DNA and the clearance of α-gal epitope were analyzed in 2 different bones (bone 1 is deproteinized bone, bone 2 is acellular and defatted bone). It was suggested that DNA of xenogeneic bone can be limited to < 50 ng per mg dry weight for the repair or regeneration with the acceptable immune risk. And α-gal clearance of xenogeneic bone could be an effective risk factor for improving xenograft quality management.ConclusionsThrough the detection of xenogeneic bone immunotoxicity, our findings indicated that the supervisions of risk factors could contribute to reduce the immune risk. And the risk factors under the acceptable limitation could decrease or replace animal experiment. However, it still needs to be studied on the limitation of α-gal epitope to predict rejection of xenogeneic bone more accurately.

show abstract

Natural and induced antibodies contribute to differential susceptibility to secondary cystic echinococcosis of Balb/c and C57Bl/6 mice

Cited by 20 publications

References 79 publications

The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model

The local immune response during Echinococcus granulosus growth in a quantitative hepatic experimental model

Echinococcus granulosus Infection Results in an Increase in Eisenbergiella and Parabacteroides Genera in the Gut of Mice

The assessment of xenogeneic bone immunotoxicity and risk management study

Contact Info

Product

Resources

About