Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization

Witzel, Claudia de Lima; Fortaleza, Carlos Magno Castelo Branco; Souza, Camila Sena Martins de; Riboli, Danilo Flávio Moraes; Cunha, Maria de Lourdes Ribeiro de Souza da

doi:10.1186/1476-0711-13-25

Cited by 9 publications

(4 citation statements)

References 23 publications

(26 reference statements)

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“…Similar results have been reported in other studies [34,35]. A low frequency of the PVL gene was also observed in other studies conducted in the region [22,35,36].…”

Section: Discussionsupporting

confidence: 92%

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Pereira-Franchi

Barreira

Costa

et al. 2019

Tropical Med Int Health

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Objective To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units (BHUs) of a Brazilian city. Methods Observational, cross‐sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterised by identifying virulence genes encoding enterotoxins A–E, haemolysins α, β and δ, exfoliatins A, B and D, biofilm production, Panton‐Valentine Leukocidin and toxic shock syndrome toxin 1, and by pulsed‐field gel electrophoresis (PFGE), multilocus sequence and spa typing. Results Eighteen methicillin‐resistant Staphylococcus aureus (MRSA) (6 SCCmec type II and 12 SCCmec type IV) and 101 (85%) MSSA were identified. PFGE typing resulted in the formation of eight clusters, with STs 1, 5, 8, 30, 188, 1176 and 1635 and spa type t002 being the predominant types among MSSA. The 18 MRSA belonged to STs 5, 8 and 1176 and spa types t002 and t062. Conclusion The results demonstrate widespread dissemination of MSSA and MRSA clones carrying haemolysin, biofilm and toxin genes. Kernel density estimation revealed the highest density of S. aureus in the 4, 5 and 8 BHUs.

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“…Similar results have been reported in other studies [34,35]. A low frequency of the PVL gene was also observed in other studies conducted in the region [22,35,36].…”

Section: Discussionsupporting

confidence: 92%

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Pereira-Franchi

Barreira

Costa

et al. 2019

Tropical Med Int Health

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“…In Brazil, the country presenting the lowest CG-MRSA burden in SSTIs, MRSA nasal colonization rates in the general population, even in at-risk populations, have been low. 54 , 55 , 56 …”

Section: Discussionmentioning

confidence: 99%

Community-genotype methicillin-resistant Staphylococcus aureus skin and soft tissue infections in Latin America: a systematic review

Leme

Bispo

Salles

2021

The Brazilian Journal of Infectious Diseases

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“…This fact reinforces the importance of throat colonization, which could be a route of transmission within the population examined. Other studies involving individuals from the same city and region also found CC5-ST5-IV and CC8-ST8-IV, suggesting that these strains are prevalent in the region [57][58][59][60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

Teixeira

Fortaleza

Souza

et al. 2021

Ann Clin Microbiol Antimicrob

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Background People with diabetes mellitus, especially insulin-dependent diabetic patients, are a risk group for staphylococcal infections. Asymptomatic infection with Staphylococcus aureus is common and favors dissemination of the microorganism, rendering these individuals a source of infection. This study aimed to characterize the resistance profile, clonal profile and sequence type, as well as to analyze the prevalence and risk factors for nasal and oropharyngeal carriage of methicillin-susceptible (MSSA) and methicillin-resistant S. aureus (MRSA) isolated from insulin-dependent diabetic individuals in the city of Botucatu, SP, Brazil. Methods Staphylococcus aureus was collected from the nasopharynx and oropharynx of 312 community-dwelling insulin-dependent diabetic individuals over a period of 3 years (October 2015 to December 2018). The isolates were characterized by susceptibility profiling, detection of the mecA gene, SCCmec typing, and molecular typing by PFGE and MLST. The risk factors associated with S. aureus and MRSA carriage were determined by logistic regression analysis. Results The overall prevalence of colonization with S. aureus and MRSA was 30.4% and 4.8%, respectively. Fifteen of the 112 S. aureus isolates carried the mecA gene; SCCmec type IV was identified in 10 isolates, SCCmec type I in three, and SCCmec type II in two. Among the 15 resistant isolates (MRSA), four were susceptible to oxacillin/cefoxitin by the disc diffusion method and one MSSA isolate was resistant to sulfamethoxazole/trimethoprim. The analysis of risk factors revealed a protective effect of age and lung disease, while lower-extremity ulcers were a risk factor for S. aureus. For MRSA, only male gender was significantly associated as a risk factor in multivariate analysis. Clonal profile analysis demonstrated the formation of clusters among MRSA isolates from different patients, with the identification of ST5-IV, ST5-I, and ST8-IV. Isolates carrying ST398 were identified among MSSA and MRSA (ST398-IV). Conclusion Our findings reinforce the importance of epidemiological studies of S. aureus carriage, especially in populations at high risk of infections such as diabetics. The data suggest widespread dissemination of MRSA in the population of insulin-dependent diabetic patients studied, as well as the emergence of important lineages among these individuals.

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Nasopharyngeal carriage of Staphylococcus aureus among imprisoned males from Brazil without exposure to healthcare: risk factors and molecular characterization

Cited by 9 publications

References 23 publications

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Community-genotype methicillin-resistant Staphylococcus aureus skin and soft tissue infections in Latin America: a systematic review

Molecular characterization of methicillin-resistant Staphylococcus aureus among insulin-dependent diabetic individuals in Brazil

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