Nasal immunization with the 40-kDa outer membrane protein of Porphyromonas gingivalis plus cholera toxin induces protective immunity in aged mice

Cai, Yu; Kurita‐Ochiai, Tomoko; Kobayashi, Ryoki; Hashizume, Tomomi; Yamamoto, Masafumi

doi:10.2334/josnusd.55.107

Cited by 17 publications

(14 citation statements)

References 36 publications

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“…In particular, we have shown the importance of the extracellular surface regions of OmpA2 in the interaction with host cells. Our data indicate a potential key role for these peptides in cellular interactions and thus suggests the exciting possibility of using surface protein-derived peptide loops as potential anti-adhesive therapeutics or immunization antigens (as has been used for other P. gingivalis proteins (Cai, Kurita-Ochiai, Kobayashi, Hashizume, & Yamamoto, 2013)) but also OmpA as a potential drug target for treatment of periodontal disease via targeting the keystone pathogen, P. gingivalis.…”

Section: Discussionmentioning

confidence: 82%

Role of OmpA2 surface regions ofPorphyromonas gingivalisin host-pathogen interactions with oral epithelial cells

et al. 2016

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Outer membrane protein A (OmpA) is a key outer membrane protein found in Gram‐negative bacteria that contributes to several crucial processes in bacterial virulence. In Porphyromonas gingivalis, OmpA is predicted as a heterotrimer of OmpA1 and OmpA2 subunits encoded by adjacent genes. Here we describe the role of OmpA and its individual subunits in the interaction of P. gingivalis with oral cells. Using knockout mutagenesis, we show that OmpA2 plays a significant role in biofilm formation and interaction with human epithelial cells. We used protein structure prediction software to identify extracellular loops of OmpA2, and determined these are involved in interactions with epithelial cells as evidenced by inhibition of adherence and invasion of P. gingivalis by synthetic extracellular loop peptides and the ability of the peptides to mediate interaction of latex beads with human cells. In particular, we observe that OmpA2‐loop 4 plays an important role in the interaction with host cells. These data demonstrate for the first time the important role of P. gingivalis OmpA2 extracellular loops in interaction with epithelial cells, which may help design novel peptide‐based antimicrobial therapies for periodontal disease.

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Section: Discussionmentioning

confidence: 82%

Role of OmpA2 surface regions ofPorphyromonas gingivalisin host-pathogen interactions with oral epithelial cells

et al. 2016

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“…While research about these toxins has been focused on their effects as mucosal adjuvants inducing Th2 immunity, their induced immunity depends on several factors, e.g., administration route and age of the animals. For instance, a non-toxic mutant of CT that in young mice induced Th2 immunity, in aged mice induced both Th1 and Th2 immunities (Cai et al, 2013). Also, it has been shown that other CT mutants induce Th17 type immunity, a strongly inflammatory response linked to some autoimmune conditions (Lee et al, 2009).…”

Section: The Adjuvant Component -Bacterial Toxinsmentioning

confidence: 98%

Alzheimer's disease vaccine development: A new strategy focusing on immune modulation

Marciani

2015

Journal of Neuroimmunology

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“…1 (CAF01) increased s-IgA in lungs, although the highest yield of sIgA was found when administering vaccines subcutaneously, followed by a booster administration intranasally. The study of Cai, et al [14] shows that by increasing the expression of IL-17A there is an increase in s-IgA levels and vice versa (i.e. with the neutralization of IL-17A there is a significant decrease in s-IgA levels).…”

Section: A B a C D Bmentioning

confidence: 99%

“…A week after the last immunization, the nose wash and nasopharyngeal tissue were isolated from the rats. [13,14]…”

Section: Immunizationmentioning

confidence: 99%

The Effect of Intranasal Immunization with Streptococcus Pilus Protein on Nasopharyngeal pIgR and IgA Expression in Rats

Mufida¹,

Handono²,

Prawiro³

et al. 2018

Turk J Immunol

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Introduction: Streptococcus pneumoniae (S. pneumoniae) causes pneumococcal disease, which has high mortality and morbidity in children under two years of age, the elderly and immunocompromised individuals. This disease can be prevented by immunization, but the current vaccine, pili protein vaccine (PPV), is less likely to protect children under the age of two and only protects against the serotypes contained in the vaccine. Hence, a new vaccine is needed to enable full protection. The use of bacterial pili proteins may offer an alternative new vaccine. Therefore, the determination of the ability of such proteins to stimulate mucosal immunity with indicator expression of pIgR and s-IgA is required. Materials and Methods: Pili were isolated using the pili bacterial cutter method, and used for nasal vaccination to the rats. TGF-β1, IL-17A, and s-IgA were measured by ELISA while pIgR was examined by immunohistochemistry. Results: This study demonstrated that intranasal immunization with antigen (54 kDa pili protein) and antigen plus adjuvant significantly increased (p<0.05) the expression of TGF-β1. However, the expression of IL-17A increased significantly (p<0.05) only in rats immunized with antigen plus adjuvant. Further analysis demonstrated that intranasal immunization with antigen and antigen plus adjuvant significantly increased (p<0.05) expression of pIgR. Expression of sIgA in nasal lavage significantly increased (p<0.05) in those rats which had been immunized with pili protein plus adjuvant. Conclusion: This study showed that the immünization with S. pneumoniae pili protein increased expression of pIgR and sIgA which are important mucosal immunity components. Therefore, this protein has potency to be developed as nasal vaccination to prevent S. pneumoniae infection.

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Nasal immunization with the 40-kDa outer membrane protein of Porphyromonas gingivalis plus cholera toxin induces protective immunity in aged mice

Cited by 17 publications

References 36 publications

Role of OmpA2 surface regions ofPorphyromonas gingivalisin host-pathogen interactions with oral epithelial cells

Role of OmpA2 surface regions ofPorphyromonas gingivalisin host-pathogen interactions with oral epithelial cells

Alzheimer's disease vaccine development: A new strategy focusing on immune modulation

The Effect of Intranasal Immunization with Streptococcus Pilus Protein on Nasopharyngeal pIgR and IgA Expression in Rats

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