Nasal Fibroblast Conditioned Medium Promotes Cell Attachment and Migration of Human Respiratory Epithelium

Man, Rohaina Che; Lokanathan, Yogeswaran; Razali, Rabiatul Adawiyah; Chowdury, Shiplu Roy; Saim, Aminuddin Bin; Idrus, Ruszymah Bt Hj

doi:10.17576/jsm-2020-4902-22

Cited by 5 publications

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“…The primary culture of human RECs and fibroblast were successfully established from nasal turbinate samples (Figure 1). In our previous studies, we successfully characterized the RECs isolated from nasal turbinate via gene expression (CK18 and 14, MUC5B, and Ki67) [32] and immunocytochemical analysis (acetylated β-tubulin, CK14, MUC5AC, and Ki67) [32,33].…”

Section: Resultsmentioning

confidence: 99%

Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering

et al. 2021

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Pathological conditions of the tracheal epithelium, such as postoperative injuries and chronic conditions, often compromise the functionality of the respiratory epithelium. Although replacement of the respiratory epithelium using various types of tracheal transplantation has been attempted, there is no predictable and dependable replacement method that holds for safe and practicable long-term use. Therefore, we used a tissue engineering approach for ex vivo regeneration of the respiratory epithelium (RE) construct. Collagen type I was isolated from sheep tendon and it was fabricated in a three-dimensional (3D) scaffold format. Isolated human respiratory epithelial cells (RECs) and fibroblasts from nasal turbinate were co-cultured on the 3D scaffold for 48 h, and epithelium maturation was allowed for another 14 days in an air–liquid interface culture system. The scanning electron microscope results revealed a fabricated porous-structure 3D collagen scaffold. The scaffold was found to be biocompatible with RECs and fibroblasts and allows cells attachment, proliferation, and migration. Immunohistochemical analysis showed that the seeded RECs and fibroblasts were positive for expression of cytokeratin 14 and collagen type I markers, respectively, indicating that the scaffold supports the native phenotype of seeded cells over a period of 14 days. Although a longer maturation period is needed for ciliogenesis to occur in RECs, the findings suggest that the tissue-engineered RE construct is a potential candidate for direct use in tracheal epithelium replacement or tracheal tube reengineering.

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Section: Resultsmentioning

confidence: 99%

Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering

et al. 2021

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“…In vitro research has investigated the effects of CM from differentiated cells on respiratory epithelium tissues. The findings suggested that CM may be used successfully to improve the functioning of both healthy and diseased cells [42,46,50]. CM derived from primary cells such as nasal fibroblasts enhance cell proliferation, attachment, and migration [42,46] and also exert a cytoprotective effect on REC [42].…”

Section: Discussionmentioning

confidence: 99%

“…The findings suggested that CM may be used successfully to improve the functioning of both healthy and diseased cells [42,46,50]. CM derived from primary cells such as nasal fibroblasts enhance cell proliferation, attachment, and migration [42,46] and also exert a cytoprotective effect on REC [42]. The CM from cell lines such as A549, when cocultured with THP-1, aids in the reduction of cancer cell metastasis [50].…”

Section: Discussionmentioning

confidence: 99%

“…While most studies examined both proliferation and migration assays, some found that a faster migration rate was often associated with a greater impact of tissue-conditioned media on cell proliferation [37,42,43]. Four studies examined cell viability assays [41,[44][45][46], where a higher proportion of viable cells was accompanied by a larger estimation of the number of live cells. The 10 studies also conducted specific marker expression experiments, namely immunofluorescence assays, PCR, ELISA, and Western blotting.…”

Section: Application Of CM From Respiratory Cells or Tissuesmentioning

confidence: 99%

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Application of Conditioned Medium for In Vitro Modeling and Repair of Respiratory Tissue

Muhammad Firdaus,

Nashihah,

Mohd Fauzi

et al. 2023

Applied Sciences

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Background: The idea of exploring respiratory therapy in vitro predominantly guided by cell-secreted substances has gained ground in recent years. A conditioned medium (CM) consists of protein milieu that contains a diverse spectrum of cytokines, chemokines, angiogenic agents, and growth factors. This review evaluated the efficacy of using CM collected in an in vitro respiratory epithelial model. Methods: Twenty-six papers were included in this review: twenty-one cellular response studies on respiratory secretome application and five studies involving animal research. Results: The CM produced by differentiated cells from respiratory and non-respiratory systems, such as mesenchymal stem cells (MSC), exhibited the similar overall effect of improving proliferation and regeneration. Not only could differentiated cells from respiratory tissues increase proliferation, migration, and attachment, but the CM was also able to protect the respiratory epithelium against cytotoxicity. Most non-respiratory tissue CM was used as a treatment model to determine the effects of the therapy, while only one study used particle-based CM and reported decreased epithelial cell tight junctions, which harmed the epithelial barrier. Conclusion: As it resolves the challenges related to cell development and wound healing while simultaneously generally reducing the danger of immunological compatibility and tumorigenicity, CM might be a potential regenerative therapy in numerous respiratory illnesses. However, additional research is required to justify using CM in respiratory epithelium clinical practice.

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Secretome Analysis of Human Nasal Fibroblast Identifies Proteins That Promote Wound Healing

Man

Idrus

Ibrahim

et al. 2023

Advances in Experimental Medicine and Biology

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Nasal Fibroblast Conditioned Medium Promotes Cell Attachment and Migration of Human Respiratory Epithelium

Cited by 5 publications

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Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering

Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering

Application of Conditioned Medium for In Vitro Modeling and Repair of Respiratory Tissue

Secretome Analysis of Human Nasal Fibroblast Identifies Proteins That Promote Wound Healing

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