Secretome Analysis of Human Nasal Fibroblast Identifies Proteins That Promote Wound Healing

Man, Rohaina Che; Idrus, Ruszymah Bt Hj; Ibrahim, Wan Izlina Wan; Saim, Aminuddin Bin; Lokanathan, Yogeswaran

doi:10.1007/5584_2023_777

Cited by 1 publication

(1 citation statement)

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“…16 Dermal FB secrete a wide variety growth factors, cytokines, and exosomes to communicate with surrounding cells to maintain and repair the extracellular matrix. 17,18 Many "growth factor" products are in fact secretome products containing exosomes. 15 Based on recent advances in understanding the role of whole cellular secretome, we now know that secreted extracellular vesicles also play an important part in cellular communication cascade.…”

Section: Fbs Secretome As a Source Of Rejuvenating Exosomesmentioning

confidence: 99%

Fibroblast‐derived growth factors and exosomes as cosmeceuticals

Mehta,

Kadoya,

Maitra

et al. 2023

Dermatological Reviews

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IntroductionFibroblast (FB)‐derived growth factors have been successfully used in skincare to improve the appearance of aged and photodamaged skin for over 20 years. Conditioned media from FB culture, used as a source of growth factors in these early products, also contain extracellular vesicles including bioactive exosomes. Exosomes are tiny extracellular vesicles that have been isolated from the secretome of human‐derived mesenchymal stem cells (MSC), adipose‐derived stem cells, or fibroblasts for potential clinical application in wound healing, skin rejuvenation, skin disease, pigmentation, and hair growth.PurposeHere, we review the potential application of dermal FB‐derived growth factors and exosomes in maintaining the structure and function of healthy skin. Specifically, we discuss how to collect and characterize FB‐derived exosomes, formulation considerations for skincare products that may contain exosomes, and provide preliminary data to support its clinical use in improving skin quality.ResultsExosomes were successfully isolated from FB secretome and characterized for their content, markers, biological activity, and clinical effects. Condition of growth medium affected exosomes compositions and properties. Product containing 98 billion EVs /mL used twice a day for 12 weeks resulted in statistically significant improvement in all skin aging parameters tested including wrinkles, roughness, sagging and laxity.ConclusionOverall, active exosomes can be identified along with growth factors in the secretome of dermal fibroblasts and may provide an additional benefit for aging skin if its biological activity remains stable in the product formulation.

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