Nasal carriage of Staphylococcus aureus in dairy sheep

Vautor, Eric; Abadie, G.; Guibert, Jean-Michel; Chevalier, Nicolas; Pépin, Michel

doi:10.1016/j.vetmic.2004.11.019

Cited by 40 publications

(35 citation statements)

References 12 publications

(10 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The carriage of S. aureus has been evaluated revealing that S. aureus colonization is common in free-living artiodactyls (Table 1). However, the carriage rates detected in the present study are lower than those reported in different domestic animals such as pigs (36%), small ruminants (from 29 to 64%), donkeys (50%), and rabbits (56%) (26)(27)(28)(29)(30)(31). In contrast to the results obtained for MRSA (17), the detection rate of MSSA in the Eurasian griffon vulture was lower than in the Iberian ibex, red deer, and wild boar, although the differences were not significant (P ϭ 0.057).…”

Section: Discussioncontrasting

confidence: 82%

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Porrero

Mentaberre

Sánchez

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

eThe presence of methicillin-susceptible Staphylococcus aureus (MSSA) was analyzed in different free-living wild animals to assess the genetic diversity and predominant genotypes on each animal species. Samples were taken from the skin and/or nares, and isolates were characterized by spa typing, multilocus sequence typing (MLST) and antimicrobial susceptibility testing. The proportion of MSSA carriers were 5.00, 22.93, 19.78, and 17.67% in Eurasian griffon vulture, Iberian ibex, red deer, and wild boar, respectively (P ‫؍‬ 0.057). A higher proportion of isolates (P ‫؍‬ 0.000) were recovered from nasal samples (78.51%) than skin samples (21.49%), but the 9.26% of red deer and 18.25% of wild boar would have been undetected if only nasal samples had been tested. Sixty-three different spa types were identified, including 25 new spa types. The most common were t528 (43.59%) in Iberian ibex, t548 and t11212 (15.79% and 14.04%) in red deer, and t3750 (36.11%) in wild boar. By MLST, 27 STs were detected, of which 12 had not been described previously. The most frequent were ST581 for Iberian ibex (48.72%), ST425 for red deer (29.82%), and ST2328 for wild boar (42.36%). Isolates from Eurasian griffon vulture belong to ST133. Host specificity has been observed for the most frequent spa types and STs (P ‫؍‬ 0.000). The highest resistance percentage was found against benzylpenicillin (average, 22.2%), although most of the S. aureus isolates were susceptible to all antimicrobial tested. Basically, MSSA isolates were different from those MRSA isolates previously detected in the same animal species.

show abstract

Section: Discussioncontrasting

confidence: 82%

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Porrero

Mentaberre

Sánchez

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…In contrast, in animals there are few studies on the colonisation of S. aureus in different body locations (Hermans et al, 2000). Studies carried out in cattle and sheep support the hypothesis that S. aureus genetic variations might also have implications for virulence capacity (Vautor et al, 2005;Stutz et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it has been reported that a substantial proportion of cases of S. aureus bacteraemia appear to be of endogenous origin, originating from colonisation of the nasal mucosa (von Eiff et al, 2001). The reported percentage of nasal carriers in different animal species is variable: 7.9% in horses (Burton et al, 2008), 29% in ewes (Vautor et al, 2005) and 32.1% in rabbits (Hermans et al, 1999). A longitudinal study in cattle observed variations in the rate of nasal carriers, increasing over time (Graveland et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus nasal carriage could be a risk for development of clinical infections in rabbits

2015

View full text Add to dashboard Cite

Although nasal carriage has been described as a risk factor for Staphylococcus aureus infections in humans, there is a scarcity of studies about S. aureus nasal carriers in animals. In rabbits, S. aureus is one of the most important pathogens responsible for a number of different types of infections. This study was designed to determine the extent of staphylococcal nasal carriage and to establish whether a relationship exists between nasal carriage and development of lesions. One hundred and sixteen rabbits with and without chronic signs of staphylococcosis from 6 industrial rabbitries were monitored. Nasal swabs for microbiological assessments were obtained from all animals. Microbiological results showed that 56% of the animals carried S. aureus in their nasal cavities with significantly higher incidence in animals with staphylococcal-related lesions (84.2%) compared to apparently healthy animals (28.8%). Additionally, the S. aureus strains isolated from the nasal cavity and lesions were clonally related in 91.7% of animals. This suggests that nasal carriage of S. aureus in rabbits could be a risk for development of clinical infections.

show abstract

“…The presence of the pathogen in raw milk represents a potential source for introduction of S. aureus into the dairy products supply chain (D'amico and Donnelly, 2010). Contamination of milk with S. aureus originates mainly from animals with intramammary infections (Dufour et al, 2012); however, other sources of contamination can be milker's hands, the farm environment, and production utensils either in ovine (Vautor et al, 2005) or caprine ( Jakobsen et al, 2011) milk.…”

Section: Introductionmentioning

confidence: 99%

Population Structure ofStaphylococcus aureusIsolated from Bulk Tank Goat's Milk

Spanu

Scarano

Virdis

et al. 2013

Foodborne Pathogens and Disease

View full text Add to dashboard Cite

The presence of Staphylococcus aureus in raw milk can represent a potential threat to human health, due to the introduction of pathogenic strains into dairy food supply chain. The present study was performed to investigate the genetic variation among S. aureus strains isolated from bulk tank goat's milk. The virulence profiles were also assessed to link the isolates with the potential source of milk contamination. A population study was performed on 60 strains using distance-based methods such as pulsed-field gel electrophoresis (PFGE), and the output was analyzed using Structure statistical software (University of Chicago; http://pritch.bsd.uchicago.edu/structure.html ). This Bayesian clustering model tool allows one to assign individuals into a population with no predefined structure. In order to assess partition of genetic variability among isolates, groups obtained by Structure were also investigated using analysis of molecular variance. S. aureus was recovered in 60 out of 78 samples (76.9%) collected from 26 farms. According to PFGE analysis, the strains were divided into 25 different pulsotypes and grouped into two main clusters. Restriction profiles, analyzed by Structure, allowed us to identify two distinct S. aureus genetic groups. Within each group, the strains showed a high coefficient of membership. A great part of genetic variability was attributable to within-groups variation. On the basis of the virulence profile, 45% of the isolates were linked to "animal" biovar, while 6.7% could be assigned to "human" biovar. Out of 60 strains, 27 were characterized by in vitro production of either enterotoxins A (5.0%), C (38.3%), or D (1.7%). The present study showed a high prevalence of bulk tank goat's milk contamination with S. aureus of animal origin. The presence in goat's milk of S. aureus strains able to produce enterotoxins and their potential introduction into dairy chain may represent a serious threat to human health.

show abstract

Nasal carriage of Staphylococcus aureus in dairy sheep

Cited by 40 publications

References 12 publications

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Carriage of Staphylococcus aureus by Free-Living Wild Animals in Spain

Staphylococcus aureus nasal carriage could be a risk for development of clinical infections in rabbits

Population Structure ofStaphylococcus aureusIsolated from Bulk Tank Goat's Milk

Contact Info

Product

Resources

About