Narrow-pore Flow Behavior of Blood Cells in Cardioplegic Suspension

Abstract

Cardioplegia alters the ionic composition of the myocardium and also the blood in a way that may influence the cellular capillary flow behavior. We measured changes in RBC volume and narrow-pore flow resistance of blood cardioplegia versus crystalloid medium. Potassium, magnesium and sodium as osmotic control caused an expected cell shrinkage and reduced the flow resistance through 3 microm pores; however, stressing the osmosis further resulted in increased resistance. No major effects were seen with the 5 microm filters. Twenty percent blood cells in the cardioplegic medium caused a 360% increase in 5 microm pore resistance. There were no obvious additional filterability effects of the cardioplegic additives other than their osmotic patterns. There may be a theoretical advantage in having a cell-free medium in terms of flow resistance. Using blood cardioplegia, a limited hypertonicity may be beneficial in reducing the capillary flow resistance of RBC.