Naringin‐inlaid silk fibroin/hydroxyapatite scaffold enhances human umbilical cord‐derived mesenchymal stem cell‐based bone regeneration

Abstract

Large bone defects caused by fracture, tumour resection and bone infection are common, debilitating clinical conditions with a substantial clinical burden. 1,2 Annually in China, over 6 million patients experience bone defects caused by trauma or various bone diseases. 3 Autologous bone grafts, allografts and artificial bone grafts are the preferred treatments for large bone defects. 4 Autologous bone grafts are the gold standard for treating bone defects. However, the bone supply is limited, and a second surgical site is required. 5 Allografts are at risk of infection and immune rejection. 6 Limitations of artificial bone grafts include osteoconductive activity, which restricts their clinical application. 7 In general, the current treatments for large bone defects remain inadequate. 8

Localized delivery of brain-derived neurotrophic factor from PLGA microspheres promotes peripheral nerve regeneration in rats

“…H&E and Toluidine blue staining were then performed to count the number of myelinated nerve fibers. Immunofluorescence (IF) of S-100 and GFAP were carried out as we described previously [ 17 ]. A total of five sections were analyzed for each sample and the mean value computed for each specimen.…”

Anisotropic silk nanofiber layers as regulators of angiogenesis for optimized bone regeneration

“…SF-based scaffolds, films and hydrogels have been developed to repair various bone defects [ 9 , [13] , [14] , [15] ]. The cytocompatibility and bioactivity of these matrices were further improved by changing nano-micro structures, anisotropic topography, degradation behavior and mechanical performance [ [16] , [17] , [18] , [19] ]. Various polymers, mineral nanomaterials, and biomacromolecules were also introduced into silk-based bone matrices, endowing them with angiogenesis and osteogenesis inducing and control features [ 5 , 20 , 21 ].…”

Traditional Chinese Medicine Compound-Loaded Materials in Bone Regeneration

“…The naringin-loaded scaffold promoted the osteogenic differentiation of hUCMSCs by activating the PI3K/Akt, VEGF, and HIF-1 signaling pathways demonstrated by gene microarray assays and enhanced bone formation at the site of reconstruction in rabbit distal femur defects, as shown by µCT and histological analyses at 4 weeks after scaffold implantation. Moreover, naringin promoted HUVEC growth and activated vascularization of SF/HA scaffolds (Zhao et al, 2021).…”