Naringin Alleviates H2O2-Inhibited Osteogenic Differentiation of Human Adipose-Derived Stromal Cells via Wnt/β-Catenin Signaling

Abstract

Osteoporosis is an age-related systemic bone disease that places a heavy burden on patients and society. In this study, we aimed to investigate the effects of naringin (NAR) on the osteogenic differentiation of human adipose-derived stromal cells (ADSCs). The results demonstrated that NAR pretreatment effectively abated H2O2-induced cell death and ROS accumulation in ADSCs undergoing osteogenic differentiation (ADSCs-OD). In addition, we also observed that the impaired extracellular matrix mineralization and ALP activity in H2O2-stimulated ADSCs-OD were notably rescued by NAR pretreatment. Moreover, the effects of H2O2 exposure on Wnt/β-catenin signaling in ADSCs-OD were largely reversed by NAR pretreatment. Collectively, our findings indicated that NAR could protect ADSCs-OD against H2O2-inhibited osteogenic differentiation.

The Development of Naringin for Use against Bone and Cartilage Disorders

“…Similarly, naringin ameliorates the H 2 O 2 -induced inhibition of osteogenic differentiation by activating the Wnt/β-catenin signaling pathways in human adipose-derived stromal cells (ADSCs) [41]. Consistently, naringin rescues H 2 O 2 -inhibited β-catenin and cyclin D1 expression, stimulates ALP activity, increases RUNX2 and OSX expression, and promotes osteogenic differentiation by inhibiting oxidative stress in human adipose-derived mesenchymal stem cells (hADMSCs) [42].…”