Narciclasine modulates polar auxin transport in Arabidopsis roots

Na, Xiaofan; Hu, Yanfeng; Yue, Kun; Lu, Hongxia; Jia, Puyou; Wang, Huahua; Wang, Xiaomin; Bi, Yurong

doi:10.1016/j.jplph.2011.01.025

Cited by 19 publications

(20 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of Arabidopsis as a good system to characterize the effects of this potential allelochemical can help us fully understand the mode of action in planta. Evidence strongly supports a significant inhibitory effect of NCS on polar auxin transport in Arabidopsis root (Na et al 2011b). Polar auxin transport has been considered to be predominantly regulated by the PIN family of auxin efflux carriers.…”

Section: Ncs Affects the Expression Of Pin And Aux1 Proteinsmentioning

confidence: 86%

See 1 more Smart Citation

Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots

et al. 2015

Planta

Self Cite

View full text Add to dashboard Cite

The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, has potential allelopathic activity and affects auxin transport. However, little is known about the cellular mechanism of this inhibitory effect of NCS on auxin transport. The present study characterizes the effects of NCS at the cellular level using transgenic Arabidopsis plants harboring the promoters of PIN, in combination with PIN-GFP proteins or AUX1-YFP fusions. NCS treatment caused significant reduction in the abundance of PIN and AUX1 proteins at the plasma membrane (PM). Analysis of the subcellular distribution of PIN and AUX1 proteins in roots revealed that NCS induced the intracellular accumulation of auxin transporters, including PIN2, PIN3, PIN4, PIN7 and AUX1. However, other PM proteins, such as PIP2, BRI1, and low temperature inducible protein 6b (LTI6b), were insensitive to NCS treatment. NCS-induced PIN2 compartments were further defined using endocytic tracer FM 4-64 labeled early endosomes and suggested that this compound affects the endocytosis trafficking of PIN proteins. Furthermore, pharmacological analysis indicated that the brefeldin A (BFA)-insensitive pathway is employed in the cellular effects of NCS on PIN2 trafficking. Although NCS did not alter actin dynamics in vitro, it resulted in the depolymerization of the actin cytoskeleton in vivo. This disruption of actin filaments by NCS subsequently influences the actin-based vesicle motility. Hence, the elucidation of the specific role of NCS is useful for further understanding the mechanisms of allelopathy at the phytohormone levels.

show abstract

Section: Ncs Affects the Expression Of Pin And Aux1 Proteinsmentioning

confidence: 86%

“…Recently, our research demonstrated that the phytotoxic effects of NCS in Arabidopsis roots involve not only the disruption of auxin signaling pathway (Hu et al 2012), but also the potent inhibitory activity against auxin transport (Na et al 2011b). However, the underlying cellular mechanism of this effect of NCS is unclear.…”

Section: Introductionmentioning

confidence: 99%

Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots

et al. 2015

Planta

Self Cite

View full text Add to dashboard Cite

show abstract

“…sesamin and kobusin), and alkaloids (e.g. sanguinarine, berberin, aristolochic acid, and narciclasine; Bailey, 1970;Trifunovic et al, 2003;Ono et al, 2006;Evidente et al, 1983;Gardiner et al, 2008;Na et al, 2011;Hu et al, 2012;Nakagawa et al, 2012;Hara and Kurita, 2014). Interestingly, the bioactivity of berberin and coptisin has been linked to the dioxole group as a structural analog, but without this functional group (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Further exploring the similarities and differences caused by the methylenedioxy group containing bioactive molecules could help in unraveling their molecular mechanism. In this perspective, it is interesting to mention narciclasine, a dioxole-containing alkaloid isolated from Narcissus tazetta bulbs (Na et al, 2011;Hu et al, 2012). Similar to MDCA, narciclasine is considered a potential allelochemical affecting postembryonic plant development by inhibiting auxin responses and modulating polar auxin transport in the target plant (Na et al, 2011;Hu et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

et al. 2016

View full text Add to dashboard Cite

The phenylpropanoid 3,4-(methylenedioxy)cinnamic acid (MDCA) is a plant-derived compound first extracted from roots of Asparagus officinalis and further characterized as an allelochemical. Later on, MDCA was identified as an efficient inhibitor of 4-COUMARATE-CoA LIGASE (4CL), a key enzyme of the general phenylpropanoid pathway. By blocking 4CL, MDCA affects the biosynthesis of many important metabolites, which might explain its phytotoxicity. To decipher the molecular basis of the allelochemical activity of MDCA, we evaluated the effect of this compound on Arabidopsis thaliana seedlings. Metabolic profiling revealed that MDCA is converted in planta into piperonylic acid (PA), an inhibitor of CINNAMATE-4-HYDROXYLASE (C4H), the enzyme directly upstream of 4CL. The inhibition of C4H was also reflected in the phenolic profile of MDCA-treated plants. Treatment of in vitro grown plants resulted in an inhibition of primary root growth and a proliferation of lateral and adventitious roots. These observed growth defects were not the consequence of lignin perturbation, but rather the result of disturbing auxin homeostasis. Based on DII-VENUS quantification and direct measurement of cellular auxin transport, we concluded that MDCA disturbs auxin gradients by interfering with auxin efflux. In addition, mass spectrometry was used to show that MDCA triggers auxin biosynthesis, conjugation, and catabolism. A similar shift in auxin homeostasis was found in the c4h mutant ref3-2, indicating that MDCA triggers a cross talk between the phenylpropanoid and auxin biosynthetic pathways independent from the observed auxin efflux inhibition. Altogether, our data provide, to our knowledge, a novel molecular explanation for the phytotoxic properties of MDCA.

show abstract

“…[22,23] It is well established that polar auxin transport (PAT) within plant tissues is regulated by auxin influx and efflux carriers, such as AUXIN RESISTANT1/LIKE AUX1 (AUX1/LAX) uptake permeases, adenosine triphosphate (ATP)-binding cassette subfamily boron (ABCB) transporters and PIN-FORMED (PIN) carrier proteins. [24,25] In plant roots, there are two PAT pathways: the first one moves IAA toward the root tip through the central cylinder cells with the aid of AUX1, ABCB19 and PIN1, PIN4 and PIN7; the second one moves IAA from the root tip toward the shoot through the outer layers of root cells with the aid of AUX1, ABCB1, PIN2 and PIN3.…”

Section: Introductionmentioning

confidence: 99%

Boron deficiency alters root growth and development and interacts with auxin metabolism by influencing the expression of auxin synthesis and transport genes

Liu

Pan

et al. 2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Boron (B) deficiency inhibits and disturbs root growth and development by interacting with auxin (indole-3-acetic acid, IAA). However, the underlying mechanism of this interaction is still poorly understood. This study found that plants in a long-term boron deprivation treatment (»0.25 mg L ¡1) had inhibited elongation of trifoliate orange roots, enlarged root tips and severe necrosis as well as a significant decrease in soluble boron and IAA content in seedling root tips. The results of a short-term boron deprivation treatment showed that a significant decrease in soluble boron and IAA content occurred after 3 hours of treatment (HOT) and 1 day of treatment (DOT), respectively. Moreover, the expression of IAA synthetic genes (TAA1, TAR2, YUC3 and YUC8) was strongly induced as early as 3 HOT and was then significantly reduced. The expression of rootward IAA transport genes (AUX1, PIN1 and PIN4) decreased significantly in the boron deprivation treatment, but the expression levels of shootward IAA transport genes (LAX1, ABCB1 and PIN3) were significantly increased. Taken together, the increase in IAA content before 1 DOT may be due to increased IAA synthesis caused by the induction of TAA1, TAR2, YUC3 and YUC8 expression; the subsequent decrease and the significantly lower final IAA content compared to the CB treatment (0.25 mg L ¡1 B) may be due to the reduced expression of IAA synthetic genes and rootward IAA transport genes and the increased expression of shootward IAA transport genes.

show abstract

Narciclasine modulates polar auxin transport in Arabidopsis roots

Cited by 19 publications

References 53 publications

Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots

Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots

The allelochemical MDCA inhibits lignification and affects auxin homeostasis

Boron deficiency alters root growth and development and interacts with auxin metabolism by influencing the expression of auxin synthesis and transport genes

Contact Info

Product

Resources

About