Narciclasine, a potential allelochemical, affects subcellular trafficking of auxin transporter proteins and actin cytoskeleton dynamics in Arabidopsis roots

Abstract

The present study documented the action of a potential allelochemical, narciclasine, on auxin transport in Arabidopsis by mainly affecting subcellular trafficking of PIN and AUX1 proteins and through interfering actin cytoskeletal organization. Narciclasine (NCS), an Amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, has potential allelopathic activity and affects auxin transport. However, little is known about the cellular mechanism of this inhibitory effect of NCS on auxin transport. The present study characterizes the effects of NCS at the cellular level using transgenic Arabidopsis plants harboring the promoters of PIN, in combination with PIN-GFP proteins or AUX1-YFP fusions. NCS treatment caused significant reduction in the abundance of PIN and AUX1 proteins at the plasma membrane (PM). Analysis of the subcellular distribution of PIN and AUX1 proteins in roots revealed that NCS induced the intracellular accumulation of auxin transporters, including PIN2, PIN3, PIN4, PIN7 and AUX1. However, other PM proteins, such as PIP2, BRI1, and low temperature inducible protein 6b (LTI6b), were insensitive to NCS treatment. NCS-induced PIN2 compartments were further defined using endocytic tracer FM 4-64 labeled early endosomes and suggested that this compound affects the endocytosis trafficking of PIN proteins. Furthermore, pharmacological analysis indicated that the brefeldin A (BFA)-insensitive pathway is employed in the cellular effects of NCS on PIN2 trafficking. Although NCS did not alter actin dynamics in vitro, it resulted in the depolymerization of the actin cytoskeleton in vivo. This disruption of actin filaments by NCS subsequently influences the actin-based vesicle motility. Hence, the elucidation of the specific role of NCS is useful for further understanding the mechanisms of allelopathy at the phytohormone levels.

Alleviation of paclitaxel-induced mechanical hypersensitivity and hyperalgesic priming with AMPK activators in male and female mice

“…Compared to other AMPK activators, narciclasine stimulates the kinase in vivo at low doses and shows efficacy in cancer, metabolic and pain models at doses that coincide with AMPK activation kinetics (Van Goietsenoven et al, 2010, Julien et al, 2017a, Julien et al, 2017b, Inyang et al, 2019b). Mechanistically, narciclasine increases the adenosine diphosphate (ADP)/ATP ratio in myotubes, which is a potential mechanism through which the compound causes AMPK activation (Hu et al, 2015, Julien et al, 2017a, Julien et al, 2017b). The mechanism through which narciclasine activates AMPK in sensory neurons is not known, but our previous work demonstrates that the drug stimulates AMPK in dorsal root ganglion neurons in vitro and in vivo (Inyang et al, 2019b).…”

The Arabidopsis Receptor Kinase ZAR1 Is Required for Zygote Asymmetric Division and Its Daughter Cell Fate

“…agb1-2 [ 65 ] was a gift from Dr. Jirong Huang at Shanghai Institute of Plant Physiology and Ecology, CAS. The seeds of EGFP-LTi6b transgenic plant [ 66 , 67 ] were from Prof. Yurong Bi at Lanzhou University.…”

Spatial regularity control of phyllotaxis pattern generated by the mutual interaction between auxin and PIN1

“…Besides, also during lateral root organogenesis, cytokinin enhances the PIN1 depletion from cell membrane to affect PIN1 polarization [ 53 – 55 ]. Furthermore, it is reported that the localization of PIN proteins is affected by diffusible molecules such as jasmonate and narciclasine [ 56 , 57 ]. It is not yet clear whether these molecules are involved in the phyllotaxis pattern or not.…”