Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm

Marco, N I Di; Páez, Paulina Laura; Estrada, Cecilia Stella Marys Lucero; Pungitore, Carlos Rodolfo

doi:10.1007/s11274-020-02971-7

Cited by 12 publications

(8 citation statements)

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“…Most bacterial species prefer to live as microcolony communities rather than single cells, since this formation behaves as a shield that keeps away the host immune response and prevents antibiotic effects. The biofilm formation of bacterial cells has highly increased the resistance to antibiotics as well as to host immune responses compared to the planktonic form of life [ 38 , 39 ]. Two important members of Staphylococci , in particular S. epidermidis and S. aureus strains, are responsible for most invasive infectious diseases because of bacterial biofilms [ 40 , 41 ].…”

Section: Resultsmentioning

confidence: 99%

“…For that reason, strong demand for searching the novel lead structures has increased in order to overcome resistance to biofilm-associated infectious diseases [ 42 ]. To date, as mentioned in the literature, the quinone scaffolds have shown the ability to disrupt formed bacterial biofilms [ 38 , 39 ] against S. aureus [ 43 ] and S. epidermidis [ 44 ]. On these bases, the potential therapeutic application of two highly active quinolinequinones ( QQ2 and QQ6 ) to disrupt bacterial biofilms was analyzed.…”

Section: Resultsmentioning

confidence: 99%

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Exploring the Relationships between Structure and Antimicrobial Potency of Quinolinequinones

et al. 2022

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Microorganisms are responsible for hospital infections, and methicillin-resistant Staphylococcus aureus is one of them. In looking for the most effective lead structures to cope with the rise of antimicrobial (antibiotic) resistance, we evaluated the antimicrobial profile of quinolinequinones for potential antimicrobial applications. 1,4-quinone molecules fused with heteroatom have been studied extensively for many years as a source of drugs and lead structures. The aims of this study were to evaluate the antimicrobial activity of quinolinequinones against bacterial and fungal strains, and to probe for potential lead structures. For this reason, the activity of these compounds against three different strains of Candida fungi (C. albicans, C. parapsilosis, and C. tropicalis) and Gram-positive and Gram-negative pathogenic bacteria were investigated, searching for potential lead compounds. Five of nine quinolinequinones showed activity mainly against the Gram-positive strains with a minimal inhibitory concentration within the Clinical and Laboratory Standards Institute (CLSI) levels. The results revealed that quinolinequinones have significant activity against bacteria including Staphylococcus aureus and Staphylococcus epidermidis, and fungi including Candida albicans and Candida parapsilosis. QQ1, QQ2, QQ3, QQ5, and QQ6 exhibited the highest growth inhibition against two essential species of the Gram-positive strains (Staphylococcus epidermidis and Staphylococcus aureus). Among these, four molecules (QQ2, QQ3, QQ5, and QQ6) were also active against Enterococcus faecalis, the other member of the Gram-positive strains. The antifungal profile of two quinolinequinones (QQ7 and QQ8) indicated that they were as effective as the reference drug Clotrimazole against Candida albicans. The same molecules also have potential inhibitory antifungal activity against Candida tropicalis. For better understanding, the most active two quinolinequinones (QQ2 and QQ6) were examined for biofilm inhibition and a time-kill kinetic study.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Exploring the Relationships between Structure and Antimicrobial Potency of Quinolinequinones

et al. 2022

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“…When lipid oxidation occurs in biofilm, the original biological fluidity will be abnormal and lead to obstacles to other related functions. The main antioxidant enzyme in the body is SOD [ 27 ]. Therefore, measuring MDA can evaluate the level of lipid oxidation in the body’s oxidative stress response, and measuring SOD activity can reflect the body’s ability to resist brain injury [ 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanism of erythropoietin-induced M2 microglia polarization via Akt / Mtor / P70S6k signaling pathway in the treatment of brain injury in premature mice and its effect on biofilm

Wang

et al. 2022

Bioengineered

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We investigated the mechanism of erythropoietin (EPO) in brain injury in premature mice based on Akt/mTOR/p70S6K signaling pathway. The brain injury model group of premature mice was obtained by intraperitoneal injection of lipopolysaccharide during pregnancy. Normal mice were taken as the control group. The model mice were divided into low-dose EPO (1,000 IU/kg, L-EPO), medium-dose EPO (2,500 IU/kg, M-EPO), and high-dose EPO groups (5,000 IU/kg, H-EPO) by intraperitoneal injection. The levels of malondialdehyde (MDA) and total superoxide dismutase (T-SOD) were detected. TUNEL staining and Western blotting were used to detect the differences in neuronal apoptosis index (AI), microglial polarization marker protein, and Akt/mTOR/p70S6K-related protein expression levels in each group. Compared with the control group, the protein levels of AI, MDA, Bax, and iNOS in the model, L-EPO, and M-EPO groups were significantly increased, while the T-SOD level and Bcl-2, ARG1, p-Akt, p-mTOR, and p-70S6K protein levels were significantly decreased ( P < 0.05). Compared with the model group, AI, MAD levels and Bax, iNOS protein expression levels in L-EPO, M-EPO, and H-EPO groups were significantly decreased, while T-SOD level and Bcl-2, ARG1, p-Akt, p-mTOR, and p-70S6K protein levels were significantly increased. The changes were dose-dependent. In summary, EPO can activate microglia transformation from M1 to M2 through Akt/mTOR/p70S6K signaling pathway.

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“…After incubation, plates were subjected to the crystal violet (CV) technique according to Di Marco et al (2021). The percent of inhibition was calculated using the equation: % I nhibition = {(OD contro l − OD sample )/OD control } × 100.…”

Section: Determination Of Minimum Biofilm Inhibitory Concentration (M...mentioning

confidence: 99%

Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus

Toranzo

Bustos

Ortega

et al. 2021

J of Applied Microbiology

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Aims To phytosynthesize silver nanoparticles (AgNPs) and determine their antibacterial and antibiofilm capacity against gram‐positive and gram‐negative bacterial strains. Methods and Results AgNPs were synthesized using Bothriochloa laguroides aqueous extract as reducing and stabilizing agent. After characterization, a phytochemical screening to the extract and the AgNPs was performed. Antibacterial activity, inhibition and eradication of biofilms against Staphylococcus aureus and Yersinia enterocolitica strains were tested. Spherical AgNPs with an average size of 8 nm were obtained. Tannins, flavonoids, carbohydrates, proanthocyanidins, anthocyanins and saponins were identified in aqueous extract; meanwhile, only carbohydrates were identified in AgNPs. The MIC and MBC were determined at pmol L−1 levels for all tested strains. Furthermore, AgNPs inhibited more than 90% of biofilms formation and eradicated more than 80% of mature biofilms at concentrations higher than MIC. Conclusions The AgNPs obtained in this study inhibited planktonic and sessile growth, and eradicated mature biofilms of pathogenic bacterial strains at very low concentrations. Significance and Impact of Study The current study showed the promising potential of AgNPs as antibiofilm agents opening the way for the future development of a new class of antibacterial products.

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Naphthoquinones inhibit formation and viability of Yersinia enterocolitica biofilm

Cited by 12 publications

References 50 publications

Exploring the Relationships between Structure and Antimicrobial Potency of Quinolinequinones

Exploring the Relationships between Structure and Antimicrobial Potency of Quinolinequinones

Mechanism of erythropoietin-induced M2 microglia polarization via Akt / Mtor / P70S6k signaling pathway in the treatment of brain injury in premature mice and its effect on biofilm

Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus

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