Naphthalene-degrading bacteria of the genus Rhodococcus from the Verkhnekamsk salt mining region of Russia

Abstract

Eight moderately halotolerant naphthalene-degrading strains of the genus Rhodococcus isolated from soil samples and slime pit bottom sediment of the Verkhnekamsk salt mining region of Russia were characterized by PCR amplification of repetitive bacterial DNA elements (rep-PCR) and identified by 16S ribosomal RNA gene sequence analysis. The diversity of their dioxygenase (nar-like) genes was investigated as these genes are known to be involved in naphthalene-degradation. The analysis of the nar-like genes identified revealed their heterogeneity in the strains under study and identity to the known sequences of nar-like genes of previously characterized from members of the genus Rhodococcus.

Halophilic Bacteria as a Promising Basis of Biopreparations for Improving the Growth of Autochthonous Strains-Destructors in Salinization Conditions

“…Of the two destructor strains of the consortium, the most effective one was included in the experimental scheme -the strain Rhodococcus sp. SMB38, which performs 100% destruction of naphthalene (0.1 g/l) in 48 hours in RMM without salt [21]. However, an increase in the osmotic pressure of the culture medium to 6% NaCl reduced the destruction of naphthalene by this culture to 31.5% in 72 hours [11].…”

Genomic, Phylogenetic and Physiological Characterization of the PAH-Degrading Strain Gordonia polyisoprenivorans 135

“…Biology 2024, 13, 339 2 of 17 Many representatives of the order Mycobacteriales of the class Actinomycetia (phylum Actinomycetota) are able to utilize PAHs. This ability has been observed in representatives of the families such as Nocardiaceae [14][15][16][17][18], Dietziaceae [19][20][21], Mycobacteriaceae [22,23] and others. Rhodococcus strains are more commonly known for their ability to degrade PAHs compared to other members of this taxonomic group [24][25][26].…”

Degradation of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 isolated from waste products of a salt-mining plant

“…Functional genes were amplified using total DNA of the strain KT112 7 [5] and the primers presented in Table 1, using a MyCy cler™ Thermal Cycler (Bio Rad Laboratories, USA) under the conditions given in the publications in Table 1 [5,[15][16][17][18][19][20][21][22][23][24]. Products of amplification were analyzed, and the results were documented as described earlier [5]. Amplicons were sequenced, and the nucleotide sequences were analyzed as described above.…”

“…In addition to exerting a degradative action against a wide range of aromatic compounds, rhodococci possess an ability to adapt to extreme living conditions [4][5][6][7]. At the same time, only single reports are known on the destruction and application of aromatic compounds as the sub strates for stains of the genus Rhodococcus under increased mineralization [4][5][6]. Due to the above stated, it is of interest to study of rhodococci isolated from ecotopes with an extreme ecological burden (including chemical contamination, increased miner alization, and climatic factors).…”