Naoxintong Protects against Atherosclerosis through Lipid-lowering and Inhibiting Maturation of Dendritic Cells in LDL Receptor Knockout Mice fed a High-fat Diet

Abstract

Naoxintong (NXT), a Chinese Materia Medica standardized product, extracted from 16 various kinds of Chinese traditional herbal medicines including Salvia miltiorrhiza, Angelica sinennsis, Astragali Radix, is clinically effective in treating atherosclerosisrelated diseases. Here, we tested the hypothesis that the anti-atherosclerosis effects of NXT might be mediated by suppressing maturation of dendritic cells (DCs) in a mice model of atherosclerosis. LDLR(-/-) mice fed a high-fat diet were treated with placebo, NXT (0.7 g/kg/d, oral diet) or simvastatin (100mg/kg/d, oral diet) for 8 weeks, respectively. NXT treatment significantly reduced plasma triglyceride (112 ± 18 mg/dl vs. 192 ± 68 mg/dl, P<0.05) and total cholesterol (944 ± 158 mg/dl vs. 1387 ± 208 mg/dl, P<0.05) compared to placebo treatment. Vascular lesions were significantly smaller and macrophage content and amount of DCs in plaques were significantly less in NXT and simvastatin groups than in placebo group (all P<0.05). In addition, expressions of splenic DC membrane molecules (CD40, CD86 and CD80) and the plasma level of IL-12p70 were significantly lower in NXT and simvastatin groups than in placebo group. In conclusion, NXT protects against atherosclerosis through lipid-lowering and inhibiting DCs maturation in this mice model of atherosclerosis.

BNC Protects H9c2 Cardiomyoblasts from H2O2-Induced Oxidative Injury through ERK1/2 Signaling Pathway

“…BNC protected mice from atherosclerosis by reducing lipid concentrations and inhibiting maturation of dendritic cells [11]. BNC also increased the catalytic activity of the drug-metabolizing CYP2C19 enzyme [12].…”

Systematic analysis of long non-coding RNA and mRNA expression changes in ApoE-deficient mice during atherosclerosis

“…Atherosclerosis is a natural process, but it may be accelerated in individuals with diabetes, hypertension, or hyperlipidemia. In the past few decades, great advances have been made in the diagnosis, prevention, and treatment of atherosclerosis; however, the precise molecular mechanisms underlying atherosclerosis remain largely unknown [4][5][6][7][8][9]. With the advent of next-generation sequencing, there is an urgent need to elucidate the genetic as well as epigenetic alterations that occur during the process of atherosclerosis.…”

Naoxintong attenuates Ischaemia/reperfusion Injury through inhibiting NLRP3 inflammasome activation

“…Further research also shown that NXT protected against atherosclerosis through lipid‐lowering and inhibiting dendritic cells (DC) maturation 9. In this study, we tested the hypothesis that NXT might attenuate myocardial I/R injury by inhibiting NLRP3 inflammasome activation.…”

“…Naoxintong (NXT), a Chinese Materia Medica standardized product extracted from 16 various kinds of Chinese traditional herbal medicines including Salvia miltiorrhiza, Angelica sinensis, Astragali Radix, could improve endothelial function and reduce infarct size in patients with AMI [8]. Further research also shown that NXT protected against atherosclerosis through lipid-lowering and inhibiting dendritic cells (DC) maturation [9]. In this study, we tested the hypothesis that NXT might attenuate myocardial I/R injury by inhibiting NLRP3 inflammasome activation.…”