BNC Protects H9c2 Cardiomyoblasts from H₂O₂-Induced Oxidative Injury through ERK1/2 Signaling Pathway

Abstract

Buchang naoxintong capsule (BNC) is a traditional Chinese medicine approved for the treatment of cerebrovascular and cardiovascular diseases. However, little is known about the specific protective function or mechanism by which BNC protects against myocardial injury. This research was designed to investigate the cardioprotective effects of BNC in vitro model of hydrogen peroxide (H2O2)-induced H9c2 rat cardiomyoblasts. BNC intestinal absorption liquid was used in this study instead of drug-containing serum or extracting solution. Our study revealed that BNC preconditioning enhanced antioxidant function by increasing the activities of total-antioxygen capacity, total-superoxide dismutase, and catalase and by decreasing the production of reactive oxygen species and malondialdehyde. BNC preconditioning also activated extracellular signal-regulated kinases (ERK1/2) and inhibited apoptosis-related proteins such as poly ADP-ribose polymerase (PARP) and caspase-3. Additionally, preincubation with BNC reduced intracellular Ca2+ concentration, improved mitochondrial membrane potential, and decreased the apoptosis rate of H9c2 cells in a dose-dependent manner. These data demonstrated that BNC protects H9c2 cardiomyoblasts from H2O2-induced oxidative injury by increasing antioxidant abilities, activating ERK1/2, and blocking Ca2+-dependent and mitochondria-mediated apoptosis. Based on our results, the potency of BNC for protecting H9c2 cells from oxidative damage is comparable to that of trimetazidine.

Xueshuan Xinmaining Tablet Treats Blood Stasis through Regulating the Expression of F13a1, Car1, and Tbxa2r

“…Buchang Naoxintong (BN) capsule, produced by Shaanxi Buchang Pharmaceutical Co., Ltd., is another traditional Chinese medicine. It has been approved for the treatment of cerebrovascular and cardiovascular diseases for many years [ 17 ]. It has the significant effects of supplementing qi, activating blood circulation, and removing blood stasis [ 18 – 21 ].…”

Naoxintong attenuates Ischaemia/reperfusion Injury through inhibiting NLRP3 inflammasome activation

“…These known NLRP3 activators induce the production of short‐lived ROS, and ROS scavengers ameliorate the activation of NLRP3 in response to different agonists 28. Previous study reported that NXT protected cardiomyocytes from H 2 O 2 ‐induced oxidative injury by enhancing antioxidant abilities 29, thus it is possible that NXT suppressed inflammasome activation at least partially through inhibition of oxidative stress.…”

Effects of short‐chain acyl‐CoA dehydrogenase on cardiomyocyte apoptosis

“…Results showed that tBHP could reduce the cell viability with some doses and time reliance. According to previous reports, the cell viability of 40–60% was used for apoptosis model . Therefore, 200 μM tBHP for 6 hrs was selected for subsequent experiments.…”