Xueshuan Xinmaining Tablet Treats Blood Stasis through Regulating the Expression of F13a1, Car1, and Tbxa2r

Abstract

Xueshuan Xinmaining Tablet (XXT), the Chinese formula, has long been administered in clinical practice for the treatment of cerebral thrombosis and coronary heart disease. In this study, we aimed to study the effect and the molecular mechanism of activating blood circulation and removing blood stasis. Rat models of cold coagulation blood stasis were induced with ice-water bath and epinephrine to assess the amelioration of blood stasis by XXT. Microarray technique was used to identify gene expression from the model and XXT-treated rats. In addition, Quantitative Real-Time PCR (qPCR) was performed to verify the microarray results. The results showed that XXT had a good therapeutic effect on blood stasis by reducing the whole blood viscosity (WBV), plasma viscosity (PV), increasing PT, APTT and TT, and by inhibiting platelet aggregation. Genes were differentially expressed in rats among the model group and the XXT-pretreated groups. XXT ameliorated blood stasis by regulating the expressions of F13a1, Car1, and Tbxa2r.

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

“…Modern medical research has shown that the occurrence of BSS is closely related to hemorheology abnormalities and coagulation disorders (Hayakawa et al, ; Szymanska, Saccenti, Smilde, & Westerhuis, ; Zhang et al, ). Our experimental results showed that the whole blood and plasma viscosity of BSS rats were significantly increased, indicating that blood running resistance was increased, which was in accordance with the previous reports (Dang et al, ; Zhang et al, ). Additionally, blood coagulation function is another crucial parameter for BSS evaluation.…”

“…Dang et al, 2015;Zhang et al, 2015). Additionally, blood coagulation function is another crucial parameter for BSS evaluation.…”

Identification of blood-activating components from Xueshuan Xinmaining Tablet based on the spectrum–effect relationship and network pharmacology analysis

“…Xueshuan Xinmaining Tablet (XXT), a Chinese traditional compound medicine composed of ten herbs, possesses the activities of promoting blood circulation and removing blood stasis. 1 It is recorded in the Chinese Pharmacopoeia (2015 edition) and has been clinically applied for the treatment of cerebral thrombosis and coronary heart disease for at least een years. 2,3 In our previous studies, we have reported that XXT could treat blood stasis through regulating related genes and proteins or endogenous metabolite pathways.…”

“…2,3 In our previous studies, we have reported that XXT could treat blood stasis through regulating related genes and proteins or endogenous metabolite pathways. 1,4,5 The multitarget mechanism is closely related to the variation in the chemical components contained. We have reported the comprehensive component screening of XXT based on UPLC/Q-TOF-MS.…”

“…So 10 kinds of XXT extract and BCN test solutions were prepared according to a dosage of 0.7 g kg À1 . 1 2.3.2 Grouping, modeling, and administration. Aer one week of adaptive feeding, 130 male Wistar rats were randomly divided into 13 groups (10 rats per group): (i) normal (N) group, (ii) model (M) group, (iii) BCN positive control group, and (ivxiii) the XXT extract (S 1 -S 10 ) groups.…”