NaOH Scarification and Stratification Improve Germination of Iris lactea var. chinensis Seed

Sun, Ying; Zhang, Y.J.; Wang, K.

doi:10.21273/hortsci.41.3.773

Cited by 12 publications

(17 citation statements)

References 8 publications

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“…Germination studies conducted on wetland and ornamental Iris species have identified cold stratification [23], [24], warm stratification [19], chemical scarification [25] and physical scarification [15] as methods of breaking dormancy. Few studies though have investigated dormancy among upland Iris species (see [26] for an exception with a desert species, I. atrofusca ).…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing Germination of a Functionally Important Grassland Plant, Iris tenax

Jones

Kaye

2014

PLoS ONE

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Grassland prairies of western Oregon and Washington are among the most endangered ecosystems in the United States. Active management and restoration are needed to promote biodiversity in the region. To support plant production for use in habitat restoration, we developed germination protocols for greenhouse propagation of Iris tenax (Oregon iris). Dormancy was most effectively overcome (63% germination) by four weeks of warm stratification at 20/30°C followed by 6–12 weeks of cold stratification at 5°C suggesting that I. tenax may have morphophysiological dormancy. This result was consistent across multiple source populations.

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Section: Introductionmentioning

confidence: 99%

Factors Influencing Germination of a Functionally Important Grassland Plant, Iris tenax

Jones

Kaye

2014

PLoS ONE

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“…We also investigated the effect of the endosperm, testa, and endocarp on germination using cut seeds. In addition, we tested seed water permeability and the effect of several treatments on seed germination, including NaOH (Crisosto and Sutter, 1985;Sun et al, 2006), gibberellic acid (GA 3 ; Chen et al, 2008Chen et al, , 2010Nicolás et al, 1996), and a moist, cold storage (cold stratification; Abe and Matsunaga, 2011; Chien et al, 1998;Sun et al, 2006;Tsuyuzaki and Miyoshi, 2009), all of which have been used to break seed dormancy in many plant species.…”

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confidence: 99%

Factors Affecting Seed Germination of Ilex latifolia and I. rotunda

Tezuka

Yokoyama

Tanaka

et al. 2013

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Mature seeds of Ilex species usually contain immature embryos and are extremely difficult to germinate. Ilex latifolia and I. rotunda, two species that are grown as ornamentals, also produce seeds that are difficult to germinate. In the present study, we investigated some factors affecting seed germination in those species. Although seeds of I. latifolia and I. rotunda could imbibe water, they did not germinate. When embryos were cultured in vitro, germination was observed in I. latifolia but not in I. rotunda. Interestingly, a transient decrease in germination frequency occurred in I. latifolia embryos isolated from seeds collected in September or October. Among five types of I. latifolia seeds that differed in the presence of the endocarp, testa, and endosperm, germinability of isolated embryos was highest. Good germination was also observed in quarter-seeds with or without endocarp followed by half-seeds. Treatment of seeds with sodium hydroxide (NaOH) had no effect on seed germination in I. latifolia. Cold stratification at 5 °C increased the germination frequency of I. latifolia embryos. In conclusion, the data suggested a mechanical barrier by the endocarp and inhibitors contained in the endosperm, testa, and/or endocarp inhibited seed germination in I. latifolia. Although no seeds or embryos of I. rotunda germinated, cold stratification in combination with other treatments deserves further investigation. Chemical name: sodium hydroxide (NaOH).

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“…In the first experiment, it was observed that the application of gibberellins and the removal of the seed coat allowed a higher percentage of germination in WL and DKL conditions. In this regards, physical seed coat wear or removal may allow water and oxygen diffusion, and eliminate or decrease the effects of the seed germination inhibitors (RAVEN et al, 2013;SUN et al, 2006). In addition, gibberellins are directly implied in the promotion of seed germination (DAVIES, 2010).…”

Section: Discussionmentioning

confidence: 99%

Towards the micropropagation of Euphorbia cyathophora Murray: a wild plant species with medicinal and ornamental potential

et al. 2019

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Euphorbia cyathophora Murray is a plant species with medicinal and ornamental potential but whose in vitro propagation is unknown. Two experiments were performed to know 1) the effect of light (WL) at 25±2 °C and 20 μmol m-2 s-1 luminous intensity, or darkness at 20±2 °C, for the first 20 days and subsequent placement in light (DKL) as previously described, and 2) the best combination of plant growth regulators: benzyl amino purine (BAP), kynetin (KIN), 2-isopentenyl adenine (2iP) and thidiazuron (TDZ) in combination with naphthaleneacetic acid (NAA) and the effect of both light conditions. The DKL treatment did not promote the growth of leaves, cotyledons, or plantlet height; in contrast, WL produced a more developed root system with early appearance of tertiary roots. In regards to the interaction of plant regulators and light effect, only the treatment with 1 mg BAP L-1 and 0.1 mg NAA L-1 generated shoots in both light conditions (DKL and WL). Darkness (DKL) generated more indirect greatest morphogenic responses (callus formation).

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NaOH Scarification and Stratification Improve Germination of Iris lactea var. chinensis Seed

Cited by 12 publications

References 8 publications

Factors Influencing Germination of a Functionally Important Grassland Plant, Iris tenax

Factors Influencing Germination of a Functionally Important Grassland Plant, Iris tenax

Factors Affecting Seed Germination of Ilex latifolia and I. rotunda

Towards the micropropagation of Euphorbia cyathophora Murray: a wild plant species with medicinal and ornamental potential

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