Nanozyme enhanced magnetic immunoassay for dual-mode detection of gastrin-17

Zheng, Chengshi; Jiang, Qixia; Wang, Kan; Li, Tangan; Zheng, Wei; Cheng, Yuemeng; Ning, Qihong; Cui, Daxiang

doi:10.1039/d2an00063f

Cited by 18 publications

(13 citation statements)

References 37 publications

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“…Immunochromatographic strips for the tests were prepared by our group. [ 14 ] The LFA strips had five components: a sample pad, a conjugate pad, an NC membrane, an absorbent pad, and a PVC backboard. The sandwich method was used to prepare these LFA strips.…”

Section: Methodsmentioning

confidence: 99%

Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay

Li,

Zheng,

et al. 2023

Biotechnology Journal

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Frequency‐mixing technology has been widely used to precisely identify magnetic nanoparticles in applications of quantitative biomedical detection in recent years. Examples include immune adsorption, lateral flow assays (LFAs), and biomagnetic imaging. However, the signals of magnetic response generated by adjacent magnetic samples interfere with each other owing to the small spacing between them in applications involving multi‐sample detection (such as the LFA and multiplexing detection). Such signal interference prevents the biosensor from obtaining characteristic peaks related to the concentration of adjacent biomarkers from the magnetic response signals. Mathematical and physical models of the structure of sensors based on frequency‐mixing techniques were developed. The theoretical model was verified and its key parameters were optimized by using simulations. A new frequency‐mixing magnetic sensor structure was then designed and developed based on the model, and the key technical problem of signal crosstalk between adjacent samples was structurally solved. Finally, standard cards with stable magnetic properties were used to evaluate the performance of the sensor, and strips of the gastrin‐17 (G‐17) LFA were used to evaluate its potential for use in clinical applications. The results show that the minimum spacing between samples required by the optimized sensor to accurately identify them was only about 4–5 mm, and the minimum detectable concentration of G‐17 was 11 pg mL−1. This is a significant reduction in the required spacing between samples for multiplexing detection. The optimized sensor also has the potential for use in multi‐channel synchronous signal acquisition, and can be used to detect synchronous magnetic signals in vivo.

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Section: Methodsmentioning

confidence: 99%

Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay

Li,

Zheng,

et al. 2023

Biotechnology Journal

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“…[ 95 ] Enhancing the sensitivity of the detection can facilitate its early diagnosis while reducing the financial burden on patients. Zheng et al [ 60 ] successfully prepared magnetic Fe 3 O 4 @Pt with POD‐like activity as probe labels for lateral flow assays (LFAs). Dual‐mode detection of G‐17 was achieved by utilizing the POD‐like activity of the nanozyme and its magnetic properties as a label, resulting in the generation of both optical and magnetic signals.…”

Section: Nanozymes Acting As Signal Amplification Probes For Sensitiv...mentioning

confidence: 99%

“…D) Schematic representation of the application of Fe 3 O 4 @Pt in magnetic LFA for G-17 detection (target enrichment and signal amplification). Reproduced with permission [60]. Copyright 2022, Royal Society of Chemistry.…”

mentioning

confidence: 99%

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Wang,

Liu,

Fan

2023

Small Methods

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Nanozymes show great promise in enhancing disease biomarker sensing by leveraging their physicochemical properties and enzymatic activities. These qualities facilitate signal amplification and matrix effects reduction, thus boosting biomarker sensing performance. In this review, recent studies from the last five years, concentrating on disease biomarker detection improvement through nanozyme‐based biosensing are examined. This enhancement primarily involves the modulations of the size, morphology, doping, modification, electromagnetic mechanisms, electron conduction efficiency, and surface plasmon resonance effects of nanozymes for increased sensitivity. In addition, a comprehensive description of the synthesis and tuning strategies employed for nanozymes has been provided. This includes a detailed elucidation of their catalytic mechanisms in alignment with the fundamental principles of enhanced sensing technology, accompanied by the presentation of quantitatively analyzed results. Moreover, the diverse applications of nanozymes in strip sensing, colorimetric sensing, electrochemical sensing, and surface‐enhanced Raman scattering have been outlined. Additionally, the limitations, challenges, and corresponding recommendations concerning the application of nanozymes in biosensing have been summarized. Furthermore, insights have been offered into the future development and outlook of nanozymes for biosensing. This review aims to serve not only as a reference for enhancing the sensitivity of nanozyme‐based biosensors but also as a catalyst for exploring nanozyme properties and their broader applications in biosensing.

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“…Probes such as colloidal gold, , fluorescent microspheres, and SERS materials were frequently utilized in the ICTs. However, fluorescence detection based on fluorescent materials is constrained by significant background noise and fluorescence quenching, whereas colloidal gold exhibits low sensitivity and a narrow dynamic range . Magnetic particles (MNPs) exhibit superior biocompatibility, stability, and ease of collection under external magnetic fields and are frequently employed in the enrichment of proteins, , nucleic acids, , rare cells, and ICTs. , The MNP-based immunochromatographic test (MICT) has been successfully employed in the quantitative detection of viruses, bacteria, and cancer biomarkers because of the high signal to noise ratio and wide linear dynamic detection range of MNPs.…”

Section: Introductionmentioning

confidence: 99%

Multiplex and Ultrasensitive Detection of Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza A/B Nucleocapsid Proteins Based on Nanometer-Sized Core–Shell Superparamagnetic Antifouling Probes

Guan

Zhang

Wang

et al. 2023

ACS Appl. Nano Mater.

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Infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza A (Flu A), and influenza B (Flu B) show similar clinical symptoms, such as cough, fever, and dyspnea, but patients infected by these viruses should be treated differently. The rapid and accurate diagnosis of infections caused by SARS-CoV-2, Flu A or Flu B is critical during the influenza season. Herein, we synthesized core−shell magnetic particles (MNPs) with excellent antifouling properties and applied them in the MNP-based immunochromatographic test (MICT) for simultaneous detection of SARS-CoV-2, Flu A, and Flu B nucleocapsid(N) proteins in 20 min. Two kinds of carboxylmodified MNPs, MNP@pMBAA and MNP@Si-SA, were prepared and evaluated as probes in the MICT. Among them, the MNP@ pMBAA showed lower nonspecific adsorption of proteins and low background noise in the application in MICTs. Particularly, the MNP@pMBAA50 bead-based MICT strip exhibited the highest signal-to-noise ratio for SARS-CoV-2 N protein detection with a limit of detection (LOD) of 0.072 ng/mL. Moreover, the proposed MICT strip demonstrated a minimal cross-reactivity and a broad linear dynamic detection range under a magnetic assay reader in the simultaneous detection of SARS-CoV-2, Flu A, and Flu B N proteins with relative LOD values of 0.0086, 0.012, and 0.018 ng/mL, respectively. The results demonstrated that the synthesized MNPs showed great potential for use as MICT probes for sensitive and multiplex detection of biomarkers in the development of point-of-care testing systems.

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Nanozyme enhanced magnetic immunoassay for dual-mode detection of gastrin-17

Abstract: In this study, we developed a novel magnetic lateral flow assay based on iron oxide decorated with platinum probes (Fe3O4@Pt) for dual-mode detection of gastrin-17 (G-17), which is one of...

Cited by 18 publications

References 37 publications

Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay

Development and optimization of a frequency mixing sensor for adjacent samples quantitative detection on a lateral flow assay

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Multiplex and Ultrasensitive Detection of Severe Acute Respiratory Syndrome Coronavirus 2 and Influenza A/B Nucleocapsid Proteins Based on Nanometer-Sized Core–Shell Superparamagnetic Antifouling Probes

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