Nanowatt simple microcalorimetry for dynamically monitoring the defense mechanism of Paramecium caudatum

The legacy of A. V. Hill's Nobel Prize winning work on muscle energetics

“… 2018 ) and chip‐based microcalorimeters (Feng et al . 2021 ). Advances in monitoring the extent of the biochemical reactions (such as fluorescent probes for ATP hydrolysis products) are also likely to enhance measurement of the time courses of biochemical changes.…”

“…However, it would be naïve to imagine that improved technology will not contribute to future developments in understanding muscle energetics. For example, there are descriptions in the recent literature of temperature-sensitive fluorescent compounds that can measure the thermal consequences of ATP splitting at the SR (Arai et al 2014), sensitive thermopiles constructed using nanofabrication techniques with novel materials (Peng et al 2018) and chip-based microcalorimeters (Feng et al 2021). Advances in monitoring the extent of the biochemical reactions (such as fluorescent probes for ATP hydrolysis products) are also likely to enhance measurement of the time courses of biochemical changes.…”

Novel Formula of Antiprotozoal Mixtures

“…Amoeba proteus was cultivated in the Prescott medium and was fed with ciliates, for example, Tetrahymena and Chilomonas [ 68 , 69 ]. Paramecium was cultivated in hay infusion [ 70 , 71 , 72 ]. Euglena was cultivated in solution according to Wu [ 73 ].…”

“…The Amoeba, Paramecium and Euglena studied in this work were isolated from the freshwater river in Krosno (river Bado, 49 Amoeba proteus was cultivated in the Prescott medium and was fed with ciliates, for example, Tetrahymena and Chilomonas [68,69]. Paramecium was cultivated in hay infusion [70][71][72]. Euglena was cultivated in solution according to Wu [73].…”

A Sub-nL Chip Calorimeter and Its Application to the Measurement of the Photothermal Transduction Efficiency of Plasmonic Nanoparticles

“…This issue has been recently settled by an oil encapsulation in a droplet-based chip calorimeter for the energy balance measurement of H2O2 decomposition catalyzed by selfpropelled Pt microrobots [14]. The technique was also used when analyzing dissipated power of Paramecium caudatum defense mechanism [15] Later calorimetry chips featured well-defined microfluidic channels and chambers for the delivery and measurement of liquids in precise volumes. A common configuration in some of those included a polydimethylsiloxane (PDMS) cover molded with channels and chamber(s) and secured over a freestanding diaphragm integrated with thin-film heaters and thermometers.…”